Formosa sp. Hel1_31_208

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Formosa; unclassified Formosa

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1SG18|A0A1H1SG18_9FLAO Uncharacterized protein OS=Formosa sp. Hel1_31_208 OX=1798225 GN=SAMN04515667_2247 PE=4 SV=1
MM1 pKa = 7.32YY2 pKa = 10.28KK3 pKa = 10.66LSICSLLILLACFDD17 pKa = 4.16VLGQTTFTKK26 pKa = 8.92TQSIALPIPGGNAPYY41 pKa = 10.39NIASGLIDD49 pKa = 4.97DD50 pKa = 5.62DD51 pKa = 4.78SYY53 pKa = 11.99PDD55 pKa = 3.44IVVSTTLGDD64 pKa = 3.43TVYY67 pKa = 10.66WLRR70 pKa = 11.84NDD72 pKa = 3.24GTGNFTLQPTPIGILDD88 pKa = 3.68SAGGVAIADD97 pKa = 3.44INGNGFNDD105 pKa = 3.69VVSTSSIDD113 pKa = 3.42GTLVWFPNDD122 pKa = 3.04GLGNFGVEE130 pKa = 4.19QPISSSLNGPGQVYY144 pKa = 9.58VRR146 pKa = 11.84AIDD149 pKa = 3.57NNSTLDD155 pKa = 3.81VAVSAYY161 pKa = 10.4NGNEE165 pKa = 4.15VVWFANDD172 pKa = 3.35GLGNFSSKK180 pKa = 10.98NIINNTIPSPGAFAMKK196 pKa = 10.4DD197 pKa = 2.75IDD199 pKa = 3.98FDD201 pKa = 4.6GDD203 pKa = 3.32IDD205 pKa = 4.47SVIANAVAFGTPNDD219 pKa = 3.5CRR221 pKa = 11.84IEE223 pKa = 4.05VFYY226 pKa = 11.44NDD228 pKa = 3.28GDD230 pKa = 4.44GNFTADD236 pKa = 3.39TNAVSVNTKK245 pKa = 10.58DD246 pKa = 3.81YY247 pKa = 9.96IFSVMAEE254 pKa = 4.16DD255 pKa = 4.04VDD257 pKa = 4.1NDD259 pKa = 3.46SSLDD263 pKa = 3.51ILVTDD268 pKa = 4.16LTGNASWFKK277 pKa = 9.85RR278 pKa = 11.84TQISPGTATYY288 pKa = 10.53SEE290 pKa = 4.47TVIPSSIANPACLDD304 pKa = 4.82LRR306 pKa = 11.84DD307 pKa = 4.75LDD309 pKa = 4.08NDD311 pKa = 3.57NLKK314 pKa = 11.01DD315 pKa = 4.83FILTSAASGSGNDD328 pKa = 3.88IIWFKK333 pKa = 11.58GDD335 pKa = 3.01GLGNFGPEE343 pKa = 4.04QIIDD347 pKa = 3.49ATQNQAYY354 pKa = 7.69TITFADD360 pKa = 4.39FEE362 pKa = 4.89NDD364 pKa = 3.06GDD366 pKa = 5.12LDD368 pKa = 4.04TATIAYY374 pKa = 10.19ADD376 pKa = 3.6DD377 pKa = 3.49RR378 pKa = 11.84VNIFRR383 pKa = 11.84NEE385 pKa = 4.14NITLSLPAIDD395 pKa = 3.46SHH397 pKa = 5.73EE398 pKa = 4.27VKK400 pKa = 10.57VYY402 pKa = 9.6PNPVSNQLYY411 pKa = 8.74IQLNTEE417 pKa = 3.88VSFSISIYY425 pKa = 10.67NSIGQLIASQTISNNNFIDD444 pKa = 3.68VSHH447 pKa = 7.1FEE449 pKa = 4.09SGVYY453 pKa = 9.82FININNYY460 pKa = 7.85AKK462 pKa = 10.15SYY464 pKa = 10.97KK465 pKa = 9.08FVKK468 pKa = 10.12YY469 pKa = 10.57

Molecular weight: 50.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1U873|A0A1H1U873_9FLAO Colanic acid/amylovoran biosynthesis glycosyltransferase OS=Formosa sp. Hel1_31_208 OX=1798225 GN=SAMN04515667_2740 PE=4 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 8.0KK42 pKa = 10.66LSVSTEE48 pKa = 3.92TRR50 pKa = 11.84HH51 pKa = 6.44KK52 pKa = 10.57KK53 pKa = 9.81

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2771

0

2771

943956

29

4178

340.7

38.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.382 ± 0.045

0.796 ± 0.025

6.013 ± 0.057

6.295 ± 0.044

5.284 ± 0.042

6.271 ± 0.047

1.852 ± 0.027

8.135 ± 0.048

7.23 ± 0.085

9.181 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.029

6.153 ± 0.051

3.321 ± 0.026

3.464 ± 0.028

3.414 ± 0.037

6.523 ± 0.038

6.173 ± 0.1

6.268 ± 0.036

1.022 ± 0.02

3.992 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski