Pseudomonas phage phiPSA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059VA49|A0A059VA49_9CAUD DNA restriction methylase OS=Pseudomonas phage phiPSA1 OX=1500757 PE=4 SV=1
MM1 pKa = 7.83PEE3 pKa = 3.94EE4 pKa = 4.14KK5 pKa = 10.57LIGPVEE11 pKa = 4.11VTRR14 pKa = 11.84DD15 pKa = 3.44EE16 pKa = 4.09NGYY19 pKa = 8.86WYY21 pKa = 10.55HH22 pKa = 6.81PGIPNFDD29 pKa = 4.0EE30 pKa = 4.42DD31 pKa = 3.67HH32 pKa = 6.61AAYY35 pKa = 9.46KK36 pKa = 10.49AWLDD40 pKa = 3.77GQQLEE45 pKa = 4.68VVGWRR50 pKa = 11.84MEE52 pKa = 4.02ADD54 pKa = 4.06LEE56 pKa = 4.36SHH58 pKa = 7.2PYY60 pKa = 8.55WEE62 pKa = 4.71EE63 pKa = 3.62GAANCLGWEE72 pKa = 4.17PEE74 pKa = 4.4KK75 pKa = 10.94PPAYY79 pKa = 10.24DD80 pKa = 3.05WFLLGIFDD88 pKa = 4.66TDD90 pKa = 3.82DD91 pKa = 3.53GPYY94 pKa = 9.29VQWAQRR100 pKa = 11.84VVTPP104 pKa = 4.74

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059VJV7|A0A059VJV7_9CAUD Uncharacterized protein OS=Pseudomonas phage phiPSA1 OX=1500757 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.15AAEE5 pKa = 4.11VLQAIEE11 pKa = 4.21GLEE14 pKa = 3.96PAAQRR19 pKa = 11.84AYY21 pKa = 10.42LAQIAQSLDD30 pKa = 3.58SVSLAEE36 pKa = 4.08VEE38 pKa = 4.19RR39 pKa = 11.84AIDD42 pKa = 3.68SGDD45 pKa = 3.3EE46 pKa = 4.27SAVVAAVQLGIFAGLVEE63 pKa = 4.81HH64 pKa = 7.24LRR66 pKa = 11.84TAYY69 pKa = 10.64VKK71 pKa = 10.57GAQTEE76 pKa = 4.02AAGIKK81 pKa = 10.17VKK83 pKa = 10.86GISKK87 pKa = 10.13EE88 pKa = 4.07LDD90 pKa = 3.21FHH92 pKa = 7.72ASGSAGFMAEE102 pKa = 3.83QASRR106 pKa = 11.84LVAQASADD114 pKa = 3.43QVVAVRR120 pKa = 11.84AVMAYY125 pKa = 10.08GSASGQSARR134 pKa = 11.84KK135 pKa = 8.03MALDD139 pKa = 4.37LIGRR143 pKa = 11.84ISKK146 pKa = 8.06QTGQRR151 pKa = 11.84TGGVLGLSGGYY162 pKa = 9.86AEE164 pKa = 5.33SVILAKK170 pKa = 9.52TQLLSGEE177 pKa = 4.04KK178 pKa = 10.6AMLRR182 pKa = 11.84RR183 pKa = 11.84YY184 pKa = 9.49LLRR187 pKa = 11.84VRR189 pKa = 11.84RR190 pKa = 11.84DD191 pKa = 2.96RR192 pKa = 11.84RR193 pKa = 11.84FDD195 pKa = 3.29PTVRR199 pKa = 11.84AAIKK203 pKa = 10.19SGKK206 pKa = 8.97PLDD209 pKa = 3.85EE210 pKa = 4.1EE211 pKa = 4.52AVNKK215 pKa = 10.03IAGRR219 pKa = 11.84YY220 pKa = 8.74ADD222 pKa = 4.96RR223 pKa = 11.84LLATQAEE230 pKa = 4.78MVAQTFVAEE239 pKa = 4.37SFNEE243 pKa = 3.86GRR245 pKa = 11.84DD246 pKa = 3.62QAWRR250 pKa = 11.84QVVARR255 pKa = 11.84SKK257 pKa = 11.1GRR259 pKa = 11.84LSFIKK264 pKa = 8.6TWKK267 pKa = 10.02SRR269 pKa = 11.84GDD271 pKa = 3.34GKK273 pKa = 10.92VRR275 pKa = 11.84YY276 pKa = 9.15SHH278 pKa = 7.12IAMNGQQVDD287 pKa = 3.24KK288 pKa = 10.07DD289 pKa = 3.79QPFVSPRR296 pKa = 11.84GALLMFPCDD305 pKa = 3.74SSLGAPLSEE314 pKa = 4.53RR315 pKa = 11.84ARR317 pKa = 11.84CRR319 pKa = 11.84CTAEE323 pKa = 3.7YY324 pKa = 10.69SIVQLRR330 pKa = 11.84II331 pKa = 3.16

Molecular weight: 35.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

13753

70

1344

264.5

28.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.121 ± 0.711

1.083 ± 0.149

5.737 ± 0.272

5.941 ± 0.274

3.294 ± 0.206

7.991 ± 0.348

1.614 ± 0.235

5.003 ± 0.198

4.893 ± 0.359

7.657 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.146

3.752 ± 0.271

4.094 ± 0.31

4.588 ± 0.375

5.89 ± 0.346

6.689 ± 0.356

6.071 ± 0.268

6.784 ± 0.244

1.578 ± 0.198

2.879 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski