Amino acid dipepetide frequency for Microbacterium sp. CGR2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.961AlaAla: 19.961 ± 0.195
0.581AlaCys: 0.581 ± 0.024
8.458AlaAsp: 8.458 ± 0.103
8.536AlaGlu: 8.536 ± 0.101
4.032AlaPhe: 4.032 ± 0.071
11.556AlaGly: 11.556 ± 0.111
2.495AlaHis: 2.495 ± 0.046
6.068AlaIle: 6.068 ± 0.072
2.669AlaLys: 2.669 ± 0.056
14.021AlaLeu: 14.021 ± 0.136
2.66AlaMet: 2.66 ± 0.055
2.254AlaAsn: 2.254 ± 0.05
6.323AlaPro: 6.323 ± 0.101
3.811AlaGln: 3.811 ± 0.058
8.921AlaArg: 8.921 ± 0.106
7.421AlaSer: 7.421 ± 0.083
7.336AlaThr: 7.336 ± 0.078
11.564AlaVal: 11.564 ± 0.129
1.849AlaTrp: 1.849 ± 0.041
2.382AlaTyr: 2.382 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.604CysAla: 0.604 ± 0.024
0.044CysCys: 0.044 ± 0.007
0.312CysAsp: 0.312 ± 0.016
0.239CysGlu: 0.239 ± 0.013
0.175CysPhe: 0.175 ± 0.01
0.534CysGly: 0.534 ± 0.022
0.12CysHis: 0.12 ± 0.009
0.204CysIle: 0.204 ± 0.015
0.051CysLys: 0.051 ± 0.007
0.405CysLeu: 0.405 ± 0.019
0.079CysMet: 0.079 ± 0.009
0.101CysAsn: 0.101 ± 0.009
0.253CysPro: 0.253 ± 0.016
0.102CysGln: 0.102 ± 0.01
0.295CysArg: 0.295 ± 0.015
0.313CysSer: 0.313 ± 0.018
0.329CysThr: 0.329 ± 0.017
0.404CysVal: 0.404 ± 0.017
0.069CysTrp: 0.069 ± 0.008
0.086CysTyr: 0.086 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
9.388AspAla: 9.388 ± 0.102
0.227AspCys: 0.227 ± 0.014
4.42AspAsp: 4.42 ± 0.072
4.483AspGlu: 4.483 ± 0.071
1.782AspPhe: 1.782 ± 0.038
6.15AspGly: 6.15 ± 0.088
1.183AspHis: 1.183 ± 0.033
2.662AspIle: 2.662 ± 0.06
1.005AspLys: 1.005 ± 0.036
6.148AspLeu: 6.148 ± 0.079
0.846AspMet: 0.846 ± 0.028
0.982AspAsn: 0.982 ± 0.03
4.036AspPro: 4.036 ± 0.057
1.604AspGln: 1.604 ± 0.04
4.406AspArg: 4.406 ± 0.062
2.777AspSer: 2.777 ± 0.052
2.957AspThr: 2.957 ± 0.051
5.609AspVal: 5.609 ± 0.076
0.934AspTrp: 0.934 ± 0.028
1.308AspTyr: 1.308 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.21GluAla: 7.21 ± 0.086
0.246GluCys: 0.246 ± 0.016
2.874GluAsp: 2.874 ± 0.055
3.317GluGlu: 3.317 ± 0.063
1.817GluPhe: 1.817 ± 0.039
4.317GluGly: 4.317 ± 0.066
1.505GluHis: 1.505 ± 0.037
3.018GluIle: 3.018 ± 0.059
1.548GluLys: 1.548 ± 0.043
6.329GluLeu: 6.329 ± 0.077
1.044GluMet: 1.044 ± 0.028
1.411GluAsn: 1.411 ± 0.033
2.916GluPro: 2.916 ± 0.056
2.282GluGln: 2.282 ± 0.047
5.148GluArg: 5.148 ± 0.076
3.309GluSer: 3.309 ± 0.058
3.284GluThr: 3.284 ± 0.06
4.696GluVal: 4.696 ± 0.072
0.993GluTrp: 0.993 ± 0.029
1.17GluTyr: 1.17 ± 0.032
0.0GluXaa: 0.0 ± 0.0
Phe
4.376PheAla: 4.376 ± 0.062
0.165PheCys: 0.165 ± 0.012
2.401PheAsp: 2.401 ± 0.048
1.77PheGlu: 1.77 ± 0.037
1.106PhePhe: 1.106 ± 0.031
3.413PheGly: 3.413 ± 0.06
0.608PheHis: 0.608 ± 0.021
1.284PheIle: 1.284 ± 0.039
0.442PheLys: 0.442 ± 0.024
3.022PheLeu: 3.022 ± 0.058
0.468PheMet: 0.468 ± 0.016
0.698PheAsn: 0.698 ± 0.027
1.468PhePro: 1.468 ± 0.032
0.805PheGln: 0.805 ± 0.026
1.884PheArg: 1.884 ± 0.046
1.763PheSer: 1.763 ± 0.038
2.208PheThr: 2.208 ± 0.042
2.823PheVal: 2.823 ± 0.055
0.517PheTrp: 0.517 ± 0.023
0.641PheTyr: 0.641 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
10.653GlyAla: 10.653 ± 0.108
0.551GlyCys: 0.551 ± 0.023
5.108GlyAsp: 5.108 ± 0.073
5.136GlyGlu: 5.136 ± 0.063
3.208GlyPhe: 3.208 ± 0.052
7.275GlyGly: 7.275 ± 0.093
1.836GlyHis: 1.836 ± 0.042
5.094GlyIle: 5.094 ± 0.067
2.075GlyLys: 2.075 ± 0.048
8.573GlyLeu: 8.573 ± 0.09
1.985GlyMet: 1.985 ± 0.042
1.758GlyAsn: 1.758 ± 0.044
3.576GlyPro: 3.576 ± 0.06
2.319GlyGln: 2.319 ± 0.046
6.198GlyArg: 6.198 ± 0.076
5.244GlySer: 5.244 ± 0.07
5.377GlyThr: 5.377 ± 0.071
7.802GlyVal: 7.802 ± 0.089
1.719GlyTrp: 1.719 ± 0.051
2.132GlyTyr: 2.132 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.482HisAla: 2.482 ± 0.055
0.112HisCys: 0.112 ± 0.009
1.351HisAsp: 1.351 ± 0.033
1.142HisGlu: 1.142 ± 0.031
0.6HisPhe: 0.6 ± 0.022
1.939HisGly: 1.939 ± 0.043
0.564HisHis: 0.564 ± 0.021
0.807HisIle: 0.807 ± 0.027
0.279HisLys: 0.279 ± 0.015
2.098HisLeu: 2.098 ± 0.045
0.35HisMet: 0.35 ± 0.017
0.362HisAsn: 0.362 ± 0.018
1.542HisPro: 1.542 ± 0.037
0.539HisGln: 0.539 ± 0.024
1.626HisArg: 1.626 ± 0.038
1.055HisSer: 1.055 ± 0.029
1.033HisThr: 1.033 ± 0.028
1.651HisVal: 1.651 ± 0.04
0.284HisTrp: 0.284 ± 0.017
0.41HisTyr: 0.41 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.365IleAla: 7.365 ± 0.084
0.26IleCys: 0.26 ± 0.015
3.645IleAsp: 3.645 ± 0.053
3.085IleGlu: 3.085 ± 0.058
1.318IlePhe: 1.318 ± 0.04
4.85IleGly: 4.85 ± 0.071
0.785IleHis: 0.785 ± 0.026
2.081IleIle: 2.081 ± 0.049
0.74IleLys: 0.74 ± 0.028
4.102IleLeu: 4.102 ± 0.07
0.693IleMet: 0.693 ± 0.027
0.957IleAsn: 0.957 ± 0.029
2.543IlePro: 2.543 ± 0.049
1.024IleGln: 1.024 ± 0.026
3.069IleArg: 3.069 ± 0.048
2.463IleSer: 2.463 ± 0.053
2.983IleThr: 2.983 ± 0.051
4.87IleVal: 4.87 ± 0.067
0.6IleTrp: 0.6 ± 0.02
0.781IleTyr: 0.781 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
2.4LysAla: 2.4 ± 0.054
0.064LysCys: 0.064 ± 0.007
1.16LysAsp: 1.16 ± 0.038
1.019LysGlu: 1.019 ± 0.034
0.461LysPhe: 0.461 ± 0.023
1.497LysGly: 1.497 ± 0.047
0.458LysHis: 0.458 ± 0.019
0.968LysIle: 0.968 ± 0.033
0.777LysLys: 0.777 ± 0.038
1.833LysLeu: 1.833 ± 0.044
0.405LysMet: 0.405 ± 0.018
0.511LysAsn: 0.511 ± 0.022
1.198LysPro: 1.198 ± 0.035
0.709LysGln: 0.709 ± 0.024
1.476LysArg: 1.476 ± 0.037
1.142LysSer: 1.142 ± 0.039
1.328LysThr: 1.328 ± 0.037
1.651LysVal: 1.651 ± 0.043
0.241LysTrp: 0.241 ± 0.015
0.44LysTyr: 0.44 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
14.045LeuAla: 14.045 ± 0.13
0.491LeuCys: 0.491 ± 0.022
6.583LeuAsp: 6.583 ± 0.079
5.087LeuGlu: 5.087 ± 0.08
2.982LeuPhe: 2.982 ± 0.056
8.962LeuGly: 8.962 ± 0.112
1.968LeuHis: 1.968 ± 0.04
4.908LeuIle: 4.908 ± 0.077
1.624LeuLys: 1.624 ± 0.043
10.181LeuLeu: 10.181 ± 0.142
1.732LeuMet: 1.732 ± 0.039
1.835LeuAsn: 1.835 ± 0.041
5.23LeuPro: 5.23 ± 0.065
2.645LeuGln: 2.645 ± 0.046
7.62LeuArg: 7.62 ± 0.085
6.108LeuSer: 6.108 ± 0.078
6.464LeuThr: 6.464 ± 0.074
8.843LeuVal: 8.843 ± 0.097
1.335LeuTrp: 1.335 ± 0.038
1.615LeuTyr: 1.615 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.133MetAla: 2.133 ± 0.042
0.079MetCys: 0.079 ± 0.008
0.856MetAsp: 0.856 ± 0.028
0.68MetGlu: 0.68 ± 0.023
0.548MetPhe: 0.548 ± 0.019
1.272MetGly: 1.272 ± 0.036
0.363MetHis: 0.363 ± 0.017
1.004MetIle: 1.004 ± 0.028
0.498MetLys: 0.498 ± 0.024
2.078MetLeu: 2.078 ± 0.044
0.392MetMet: 0.392 ± 0.019
0.537MetAsn: 0.537 ± 0.019
1.219MetPro: 1.219 ± 0.03
0.563MetGln: 0.563 ± 0.018
1.474MetArg: 1.474 ± 0.033
1.55MetSer: 1.55 ± 0.038
1.758MetThr: 1.758 ± 0.039
1.381MetVal: 1.381 ± 0.034
0.204MetTrp: 0.204 ± 0.012
0.248MetTyr: 0.248 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.553AsnAla: 2.553 ± 0.058
0.094AsnCys: 0.094 ± 0.008
1.255AsnAsp: 1.255 ± 0.036
1.087AsnGlu: 1.087 ± 0.03
0.617AsnPhe: 0.617 ± 0.025
1.975AsnGly: 1.975 ± 0.052
0.355AsnHis: 0.355 ± 0.018
0.888AsnIle: 0.888 ± 0.029
0.382AsnLys: 0.382 ± 0.022
1.887AsnLeu: 1.887 ± 0.038
0.356AsnMet: 0.356 ± 0.018
0.462AsnAsn: 0.462 ± 0.022
1.595AsnPro: 1.595 ± 0.042
0.576AsnGln: 0.576 ± 0.024
1.33AsnArg: 1.33 ± 0.033
1.042AsnSer: 1.042 ± 0.03
1.167AsnThr: 1.167 ± 0.034
1.669AsnVal: 1.669 ± 0.036
0.34AsnTrp: 0.34 ± 0.017
0.451AsnTyr: 0.451 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
6.696ProAla: 6.696 ± 0.091
0.179ProCys: 0.179 ± 0.012
3.81ProAsp: 3.81 ± 0.06
3.89ProGlu: 3.89 ± 0.066
1.695ProPhe: 1.695 ± 0.036
4.714ProGly: 4.714 ± 0.073
1.124ProHis: 1.124 ± 0.031
2.148ProIle: 2.148 ± 0.042
1.015ProLys: 1.015 ± 0.038
4.918ProLeu: 4.918 ± 0.069
0.875ProMet: 0.875 ± 0.024
1.028ProAsn: 1.028 ± 0.029
2.213ProPro: 2.213 ± 0.05
1.556ProGln: 1.556 ± 0.04
3.4ProArg: 3.4 ± 0.054
3.182ProSer: 3.182 ± 0.056
3.389ProThr: 3.389 ± 0.052
4.648ProVal: 4.648 ± 0.06
0.871ProTrp: 0.871 ± 0.029
0.997ProTyr: 0.997 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.483GlnAla: 3.483 ± 0.056
0.099GlnCys: 0.099 ± 0.009
1.342GlnAsp: 1.342 ± 0.035
1.447GlnGlu: 1.447 ± 0.034
0.887GlnPhe: 0.887 ± 0.029
2.203GlnGly: 2.203 ± 0.044
0.682GlnHis: 0.682 ± 0.022
1.486GlnIle: 1.486 ± 0.041
0.687GlnLys: 0.687 ± 0.023
3.106GlnLeu: 3.106 ± 0.053
0.547GlnMet: 0.547 ± 0.023
0.709GlnAsn: 0.709 ± 0.026
1.444GlnPro: 1.444 ± 0.041
1.137GlnGln: 1.137 ± 0.039
2.449GlnArg: 2.449 ± 0.051
1.507GlnSer: 1.507 ± 0.036
1.602GlnThr: 1.602 ± 0.035
2.32GlnVal: 2.32 ± 0.044
0.473GlnTrp: 0.473 ± 0.02
0.57GlnTyr: 0.57 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
8.713ArgAla: 8.713 ± 0.103
0.294ArgCys: 0.294 ± 0.017
4.375ArgAsp: 4.375 ± 0.062
4.42ArgGlu: 4.42 ± 0.071
2.413ArgPhe: 2.413 ± 0.044
5.435ArgGly: 5.435 ± 0.084
1.612ArgHis: 1.612 ± 0.039
3.731ArgIle: 3.731 ± 0.063
1.377ArgLys: 1.377 ± 0.035
7.092ArgLeu: 7.092 ± 0.09
1.954ArgMet: 1.954 ± 0.042
1.399ArgAsn: 1.399 ± 0.033
3.556ArgPro: 3.556 ± 0.064
2.086ArgGln: 2.086 ± 0.044
6.611ArgArg: 6.611 ± 0.091
4.305ArgSer: 4.305 ± 0.067
4.464ArgThr: 4.464 ± 0.069
5.983ArgVal: 5.983 ± 0.079
1.251ArgTrp: 1.251 ± 0.034
1.554ArgTyr: 1.554 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
7.435SerAla: 7.435 ± 0.095
0.26SerCys: 0.26 ± 0.018
3.44SerAsp: 3.44 ± 0.059
3.065SerGlu: 3.065 ± 0.054
2.02SerPhe: 2.02 ± 0.044
5.783SerGly: 5.783 ± 0.08
1.063SerHis: 1.063 ± 0.032
2.752SerIle: 2.752 ± 0.051
1.144SerLys: 1.144 ± 0.033
5.53SerLeu: 5.53 ± 0.06
1.248SerMet: 1.248 ± 0.034
1.103SerAsn: 1.103 ± 0.03
3.104SerPro: 3.104 ± 0.049
1.376SerGln: 1.376 ± 0.039
3.932SerArg: 3.932 ± 0.059
3.569SerSer: 3.569 ± 0.071
3.724SerThr: 3.724 ± 0.056
4.799SerVal: 4.799 ± 0.075
1.019SerTrp: 1.019 ± 0.027
1.11SerTyr: 1.11 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
8.01ThrAla: 8.01 ± 0.089
0.26ThrCys: 0.26 ± 0.02
3.734ThrAsp: 3.734 ± 0.064
3.239ThrGlu: 3.239 ± 0.052
1.985ThrPhe: 1.985 ± 0.047
5.666ThrGly: 5.666 ± 0.072
1.162ThrHis: 1.162 ± 0.032
2.978ThrIle: 2.978 ± 0.058
1.239ThrLys: 1.239 ± 0.038
6.053ThrLeu: 6.053 ± 0.077
1.053ThrMet: 1.053 ± 0.03
1.205ThrAsn: 1.205 ± 0.033
3.83ThrPro: 3.83 ± 0.063
1.626ThrGln: 1.626 ± 0.042
3.847ThrArg: 3.847 ± 0.06
3.463ThrSer: 3.463 ± 0.067
3.899ThrThr: 3.899 ± 0.077
5.78ThrVal: 5.78 ± 0.085
0.881ThrTrp: 0.881 ± 0.027
1.178ThrTyr: 1.178 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
11.344ValAla: 11.344 ± 0.118
0.502ValCys: 0.502 ± 0.022
5.705ValAsp: 5.705 ± 0.07
4.947ValGlu: 4.947 ± 0.066
2.912ValPhe: 2.912 ± 0.053
7.142ValGly: 7.142 ± 0.1
1.695ValHis: 1.695 ± 0.039
4.603ValIle: 4.603 ± 0.069
1.535ValLys: 1.535 ± 0.041
9.119ValLeu: 9.119 ± 0.111
1.514ValMet: 1.514 ± 0.034
1.851ValAsn: 1.851 ± 0.049
4.603ValPro: 4.603 ± 0.063
2.297ValGln: 2.297 ± 0.044
6.084ValArg: 6.084 ± 0.083
5.156ValSer: 5.156 ± 0.058
5.617ValThr: 5.617 ± 0.075
8.53ValVal: 8.53 ± 0.109
1.145ValTrp: 1.145 ± 0.027
1.475ValTyr: 1.475 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.632TrpAla: 1.632 ± 0.036
0.105TrpCys: 0.105 ± 0.009
0.834TrpAsp: 0.834 ± 0.027
0.708TrpGlu: 0.708 ± 0.024
0.595TrpPhe: 0.595 ± 0.022
1.126TrpGly: 1.126 ± 0.037
0.356TrpHis: 0.356 ± 0.016
0.758TrpIle: 0.758 ± 0.027
0.328TrpLys: 0.328 ± 0.018
1.724TrpLeu: 1.724 ± 0.041
0.369TrpMet: 0.369 ± 0.016
0.475TrpAsn: 0.475 ± 0.02
0.717TrpPro: 0.717 ± 0.028
0.57TrpGln: 0.57 ± 0.024
1.27TrpArg: 1.27 ± 0.033
0.975TrpSer: 0.975 ± 0.03
0.987TrpThr: 0.987 ± 0.035
1.21TrpVal: 1.21 ± 0.035
0.382TrpTrp: 0.382 ± 0.019
0.29TrpTyr: 0.29 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.415TyrAla: 2.415 ± 0.046
0.105TyrCys: 0.105 ± 0.01
1.247TyrAsp: 1.247 ± 0.036
1.138TyrGlu: 1.138 ± 0.033
0.71TyrPhe: 0.71 ± 0.026
1.811TyrGly: 1.811 ± 0.036
0.291TyrHis: 0.291 ± 0.015
0.772TyrIle: 0.772 ± 0.028
0.321TyrLys: 0.321 ± 0.017
2.04TyrLeu: 2.04 ± 0.047
0.266TyrMet: 0.266 ± 0.014
0.442TyrAsn: 0.442 ± 0.02
0.98TyrPro: 0.98 ± 0.031
0.554TyrGln: 0.554 ± 0.021
1.586TyrArg: 1.586 ± 0.036
1.131TyrSer: 1.131 ± 0.032
1.183TyrThr: 1.183 ± 0.035
1.543TyrVal: 1.543 ± 0.038
0.304TyrTrp: 0.304 ± 0.014
0.462TyrTyr: 0.462 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3832 proteins (1195693 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski