Jeotgalibacillus sp. R-1-5s-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Jeotgalibacillus; unclassified Jeotgalibacillus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8LKF2|A0A4Y8LKF2_9BACL Outer spore coat protein CotE OS=Jeotgalibacillus sp. R-1-5s-1 OX=2555897 GN=cotE PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.64KK3 pKa = 9.28WSKK6 pKa = 9.89AASGIVMVSSAVVLGACGNTSGSADD31 pKa = 3.61EE32 pKa = 4.91PIIVAGKK39 pKa = 8.68PWTEE43 pKa = 3.98QYY45 pKa = 10.75ILPHH49 pKa = 7.54ILEE52 pKa = 5.02LYY54 pKa = 10.27IEE56 pKa = 4.79GNSEE60 pKa = 4.16YY61 pKa = 10.35EE62 pKa = 4.13VEE64 pKa = 4.12LEE66 pKa = 4.04EE67 pKa = 4.89GLGEE71 pKa = 4.26VAILTPSIEE80 pKa = 4.18NGDD83 pKa = 2.66IDD85 pKa = 4.15MYY87 pKa = 11.37VEE89 pKa = 3.94YY90 pKa = 10.74SGTGLEE96 pKa = 4.12AVLNEE101 pKa = 4.47KK102 pKa = 10.37ADD104 pKa = 3.6QGEE107 pKa = 4.26SSEE110 pKa = 4.77SILEE114 pKa = 4.14RR115 pKa = 11.84VRR117 pKa = 11.84EE118 pKa = 4.63GYY120 pKa = 8.54EE121 pKa = 3.57EE122 pKa = 4.64RR123 pKa = 11.84YY124 pKa = 9.03EE125 pKa = 4.32VAWLEE130 pKa = 3.88PLGFEE135 pKa = 4.77NSYY138 pKa = 9.82TMAYY142 pKa = 9.87AADD145 pKa = 3.79SGITATTYY153 pKa = 10.51TEE155 pKa = 3.91LAGVSNEE162 pKa = 4.16LVFGAPHH169 pKa = 6.37SFYY172 pKa = 10.76EE173 pKa = 4.5RR174 pKa = 11.84EE175 pKa = 3.91GDD177 pKa = 3.79GYY179 pKa = 11.24DD180 pKa = 3.87ALVEE184 pKa = 4.2AYY186 pKa = 9.95GYY188 pKa = 10.02EE189 pKa = 4.17FTSAEE194 pKa = 4.17SFDD197 pKa = 3.63ASIMYY202 pKa = 9.93DD203 pKa = 3.14AVRR206 pKa = 11.84TGQVDD211 pKa = 4.24VIPAFTTDD219 pKa = 2.89GRR221 pKa = 11.84IEE223 pKa = 4.24RR224 pKa = 11.84FNLVTTEE231 pKa = 4.34DD232 pKa = 3.9DD233 pKa = 3.88LGFFPKK239 pKa = 10.26YY240 pKa = 9.9DD241 pKa = 3.33AVPIVRR247 pKa = 11.84MDD249 pKa = 3.22ILEE252 pKa = 5.0DD253 pKa = 3.72YY254 pKa = 10.76PEE256 pKa = 5.25LEE258 pKa = 3.98EE259 pKa = 5.09VMNGLAGQISDD270 pKa = 4.94EE271 pKa = 4.42EE272 pKa = 4.18MQEE275 pKa = 3.49MNARR279 pKa = 11.84VDD281 pKa = 3.15IDD283 pKa = 3.64GDD285 pKa = 3.75DD286 pKa = 3.82YY287 pKa = 11.3RR288 pKa = 11.84TVAQEE293 pKa = 3.79FLQSKK298 pKa = 9.86GLIEE302 pKa = 4.08EE303 pKa = 4.35

Molecular weight: 33.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8L241|A0A4Y8L241_9BACL Uncharacterized protein OS=Jeotgalibacillus sp. R-1-5s-1 OX=2555897 GN=E2491_10890 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.36VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.8VLSAA44 pKa = 4.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3369

0

3369

982630

27

1545

291.7

32.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.541 ± 0.047

0.626 ± 0.012

5.409 ± 0.037

7.955 ± 0.049

4.521 ± 0.035

7.007 ± 0.042

2.176 ± 0.021

7.203 ± 0.045

6.103 ± 0.034

9.753 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.886 ± 0.019

3.857 ± 0.03

3.64 ± 0.024

3.859 ± 0.027

4.363 ± 0.031

6.168 ± 0.029

5.44 ± 0.027

7.096 ± 0.033

1.053 ± 0.016

3.343 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski