Tortoise microvirus 47

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1FVY9|A0A4V1FVY9_9VIRU Uncharacterized protein OS=Tortoise microvirus 47 OX=2583151 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84RR3 pKa = 11.84QSARR7 pKa = 11.84GITSLPAGKK16 pKa = 8.49MVPIAAFPLLRR27 pKa = 11.84EE28 pKa = 3.94DD29 pKa = 4.99AIRR32 pKa = 11.84SGRR35 pKa = 11.84LRR37 pKa = 11.84FSFEE41 pKa = 3.98SMEE44 pKa = 4.11TAEE47 pKa = 4.51ILMNAINVCVMAYY60 pKa = 9.8LVPNLALEE68 pKa = 4.31RR69 pKa = 11.84FNGSMDD75 pKa = 3.71QLNLSYY81 pKa = 10.44EE82 pKa = 4.33GKK84 pKa = 9.65PPLDD88 pKa = 4.11GEE90 pKa = 4.45PVTPYY95 pKa = 10.43IEE97 pKa = 4.08TAPFGAHH104 pKa = 5.56GTNDD108 pKa = 2.59IYY110 pKa = 11.08TYY112 pKa = 10.7LGLHH116 pKa = 6.34GRR118 pKa = 11.84PTQQVNTAYY127 pKa = 10.85LEE129 pKa = 4.4AYY131 pKa = 8.74NCIWNFRR138 pKa = 11.84AKK140 pKa = 10.35NRR142 pKa = 11.84SKK144 pKa = 10.61EE145 pKa = 3.8LSEE148 pKa = 4.05RR149 pKa = 11.84ARR151 pKa = 11.84LQTDD155 pKa = 4.22LAPAFWSHH163 pKa = 7.31DD164 pKa = 3.76MFKK167 pKa = 10.67HH168 pKa = 5.57IVPDD172 pKa = 4.2FDD174 pKa = 3.28QAAIDD179 pKa = 4.41GEE181 pKa = 4.69VPLNITDD188 pKa = 2.68GRR190 pKa = 11.84MPVRR194 pKa = 11.84GIGLGGTTSIATNSAVRR211 pKa = 11.84EE212 pKa = 4.25SGGTATYY219 pKa = 10.89ARR221 pKa = 11.84STTTAVDD228 pKa = 3.46TGQSVRR234 pKa = 11.84LQLAATGTGTTVLPDD249 pKa = 3.34IYY251 pKa = 11.34AEE253 pKa = 4.04MEE255 pKa = 4.15EE256 pKa = 4.11NGITVSLSNIEE267 pKa = 4.22LARR270 pKa = 11.84KK271 pKa = 6.35TQAFAKK277 pKa = 10.47LRR279 pKa = 11.84TQYY282 pKa = 10.49NAHH285 pKa = 7.39DD286 pKa = 3.67DD287 pKa = 4.12WIINLLMDD295 pKa = 5.67GISIPEE301 pKa = 3.87QAFKK305 pKa = 11.11QPMLLAEE312 pKa = 4.29KK313 pKa = 9.66RR314 pKa = 11.84TIFGMAKK321 pKa = 9.85RR322 pKa = 11.84YY323 pKa = 10.23ASDD326 pKa = 3.33SGNLTEE332 pKa = 5.04SVVNGATFLDD342 pKa = 4.23MSIQLPRR349 pKa = 11.84IGTGGVVMIVAEE361 pKa = 4.15ITPDD365 pKa = 3.3QLFEE369 pKa = 4.15RR370 pKa = 11.84QEE372 pKa = 3.86DD373 pKa = 4.71PYY375 pKa = 11.75LHH377 pKa = 5.67TTNVEE382 pKa = 3.78QLPQYY387 pKa = 10.93LRR389 pKa = 11.84DD390 pKa = 3.58TLDD393 pKa = 3.47PEE395 pKa = 4.42KK396 pKa = 11.1VDD398 pKa = 3.03IVKK401 pKa = 10.6NGRR404 pKa = 11.84IDD406 pKa = 3.54VDD408 pKa = 3.37HH409 pKa = 7.37DD410 pKa = 3.75QPDD413 pKa = 3.28ATFGYY418 pKa = 10.29EE419 pKa = 3.87PLNARR424 pKa = 11.84WNINAPRR431 pKa = 11.84IGARR435 pKa = 11.84FYY437 pKa = 10.94RR438 pKa = 11.84PEE440 pKa = 3.54VDD442 pKa = 4.19AGFDD446 pKa = 3.54EE447 pKa = 4.48DD448 pKa = 4.37RR449 pKa = 11.84QRR451 pKa = 11.84IWAVEE456 pKa = 4.24TKK458 pKa = 10.68NPTLSTDD465 pKa = 5.09FYY467 pKa = 11.08LCTNMHH473 pKa = 6.47TKK475 pKa = 10.32PFVVTNQDD483 pKa = 2.92PFEE486 pKa = 4.31VVTQGDD492 pKa = 3.9VFIEE496 pKa = 4.52GNTVFGGHH504 pKa = 7.35LIEE507 pKa = 5.26ATDD510 pKa = 4.15DD511 pKa = 3.9YY512 pKa = 11.6EE513 pKa = 4.91KK514 pKa = 11.21VLAVADD520 pKa = 3.48QTRR523 pKa = 11.84IEE525 pKa = 4.28KK526 pKa = 9.86EE527 pKa = 3.86QAA529 pKa = 2.85

Molecular weight: 58.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVY9|A0A4V1FVY9_9VIRU Uncharacterized protein OS=Tortoise microvirus 47 OX=2583151 PE=4 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84KK4 pKa = 9.47NRR6 pKa = 11.84TRR8 pKa = 11.84RR9 pKa = 11.84SKK11 pKa = 10.64AAASRR16 pKa = 11.84GAKK19 pKa = 9.15GKK21 pKa = 9.76NAASVQNRR29 pKa = 11.84DD30 pKa = 3.28ARR32 pKa = 11.84SLAASPLAFMRR43 pKa = 11.84AATIKK48 pKa = 10.63SPTRR52 pKa = 11.84AKK54 pKa = 10.3SVNVPRR60 pKa = 11.84RR61 pKa = 11.84QIVEE65 pKa = 3.96IEE67 pKa = 4.64LPPAPIIQEE76 pKa = 3.98NKK78 pKa = 8.9RR79 pKa = 11.84ARR81 pKa = 11.84SALALKK87 pKa = 10.38AISEE91 pKa = 4.06PDD93 pKa = 3.32NRR95 pKa = 11.84KK96 pKa = 9.7SSPKK100 pKa = 10.15ARR102 pKa = 11.84EE103 pKa = 3.53QLHH106 pKa = 5.91CKK108 pKa = 9.61KK109 pKa = 10.7RR110 pKa = 11.84PDD112 pKa = 3.53SKK114 pKa = 10.92KK115 pKa = 10.13AATGKK120 pKa = 10.58GGGVKK125 pKa = 10.22RR126 pKa = 11.84FIPWCGG132 pKa = 2.95

Molecular weight: 14.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1702

132

529

283.7

31.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.694 ± 1.091

0.881 ± 0.301

5.464 ± 0.681

6.816 ± 0.568

3.584 ± 0.34

8.049 ± 0.677

2.115 ± 0.376

4.524 ± 0.614

4.994 ± 1.112

6.404 ± 0.645

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.703 ± 0.166

4.348 ± 0.461

6.345 ± 0.354

4.583 ± 0.262

7.462 ± 0.886

5.112 ± 0.819

5.699 ± 1.08

5.993 ± 0.69

1.939 ± 0.542

3.29 ± 0.858
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski