Jamestown Canyon virus
Average proteome isoelectric point is 7.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2EZQ0|E2EZQ0_9VIRU Replicase OS=Jamestown Canyon virus OX=35511 PE=4 SV=1
MM1 pKa = 8.19 DD2 pKa = 3.66 NTEE5 pKa = 4.06 YY6 pKa = 10.79 QQFLARR12 pKa = 11.84 INAARR17 pKa = 11.84 DD18 pKa = 3.13 ACVAKK23 pKa = 10.75 DD24 pKa = 3.37 IDD26 pKa = 4.11 VDD28 pKa = 4.25 LLMARR33 pKa = 11.84 HH34 pKa = 6.75 DD35 pKa = 4.06 YY36 pKa = 10.43 FGKK39 pKa = 10.17 EE40 pKa = 3.76 LCKK43 pKa = 10.57 SLNIEE48 pKa = 4.1 YY49 pKa = 10.56 RR50 pKa = 11.84 NDD52 pKa = 3.16 IPFVDD57 pKa = 3.79 ILLDD61 pKa = 3.51 IRR63 pKa = 11.84 PDD65 pKa = 3.55 IDD67 pKa = 4.8 PLTLDD72 pKa = 4.41 IPHH75 pKa = 6.98 ITPDD79 pKa = 3.0 NYY81 pKa = 11.16 LYY83 pKa = 10.81 LDD85 pKa = 4.45 NILYY89 pKa = 10.25 IIDD92 pKa = 3.84 YY93 pKa = 9.29 KK94 pKa = 11.36 VSVSNEE100 pKa = 3.38 SSIITNTKK108 pKa = 7.9 YY109 pKa = 10.69 FEE111 pKa = 4.1 MTRR114 pKa = 11.84 DD115 pKa = 3.3 IASVLGLHH123 pKa = 6.42 IEE125 pKa = 4.02 IVIIRR130 pKa = 11.84 IDD132 pKa = 4.29 PISRR136 pKa = 11.84 DD137 pKa = 3.59 LYY139 pKa = 10.07 ISSDD143 pKa = 3.23 RR144 pKa = 11.84 FKK146 pKa = 11.41 DD147 pKa = 3.65 LFPTLVVDD155 pKa = 4.06 INFNQFFDD163 pKa = 4.41 LKK165 pKa = 10.1 QALYY169 pKa = 10.64 EE170 pKa = 4.06 KK171 pKa = 10.65 FGDD174 pKa = 4.51 DD175 pKa = 4.45 EE176 pKa = 4.5 EE177 pKa = 5.01 FLLKK181 pKa = 10.23 VAHH184 pKa = 7.15 GDD186 pKa = 3.73 FTLTAPWCKK195 pKa = 9.59 TGCPQVWQHH204 pKa = 6.73 PIYY207 pKa = 10.81 KK208 pKa = 9.47 EE209 pKa = 4.28 FKK211 pKa = 9.39 LSMPIPEE218 pKa = 3.6 RR219 pKa = 11.84 RR220 pKa = 11.84 LFEE223 pKa = 3.98 EE224 pKa = 3.98 SMKK227 pKa = 10.45 FNSYY231 pKa = 9.82 EE232 pKa = 4.03 SEE234 pKa = 3.75 RR235 pKa = 11.84 WNTNLIKK242 pKa = 10.26 IRR244 pKa = 11.84 EE245 pKa = 4.2 YY246 pKa = 10.41 TKK248 pKa = 10.73 KK249 pKa = 10.25 EE250 pKa = 3.8 YY251 pKa = 11.0 NDD253 pKa = 5.27 FITKK257 pKa = 9.21 SAKK260 pKa = 9.83 EE261 pKa = 3.98 VFLATGNYY269 pKa = 7.69 KK270 pKa = 10.07 QPNKK274 pKa = 10.72 SEE276 pKa = 3.94 ILEE279 pKa = 4.21 GWEE282 pKa = 3.72 MMVEE286 pKa = 4.18 RR287 pKa = 11.84 VHH289 pKa = 5.86 QQRR292 pKa = 11.84 EE293 pKa = 4.17 VSNSIHH299 pKa = 5.89 DD300 pKa = 4.03 QKK302 pKa = 11.2 PSIHH306 pKa = 6.62 FIWSTHH312 pKa = 5.42 NSNNSNNATFKK323 pKa = 11.09 LILLSKK329 pKa = 10.05 SLQSIKK335 pKa = 10.67 GSSTYY340 pKa = 10.16 TEE342 pKa = 4.28 SFKK345 pKa = 11.4 SLGKK349 pKa = 10.34 MMDD352 pKa = 3.24 IGDD355 pKa = 5.0 KK356 pKa = 9.16 ITDD359 pKa = 3.85 YY360 pKa = 11.06 EE361 pKa = 4.48 SHH363 pKa = 7.24 CDD365 pKa = 3.71 YY366 pKa = 11.32 LKK368 pKa = 9.41 TQARR372 pKa = 11.84 LSWRR376 pKa = 11.84 QVMNKK381 pKa = 9.68 KK382 pKa = 10.47 LEE384 pKa = 4.16 PKK386 pKa = 9.89 KK387 pKa = 11.0 INDD390 pKa = 3.94 ALVLWEE396 pKa = 3.91 QQFMVNSEE404 pKa = 5.01 LINKK408 pKa = 8.81 NDD410 pKa = 3.43 KK411 pKa = 10.98 LKK413 pKa = 10.9 LFRR416 pKa = 11.84 DD417 pKa = 4.36 FCGIGKK423 pKa = 9.22 HH424 pKa = 5.17 KK425 pKa = 10.41 QFKK428 pKa = 10.88 NKK430 pKa = 8.69 MLEE433 pKa = 4.45 DD434 pKa = 3.87 IEE436 pKa = 4.3 LSKK439 pKa = 11.07 PKK441 pKa = 10.28 ILDD444 pKa = 3.74 FNDD447 pKa = 3.07 EE448 pKa = 4.34 NIYY451 pKa = 10.45 LASLTMIEE459 pKa = 4.0 QCKK462 pKa = 10.3 IMLSEE467 pKa = 4.07 QSGLKK472 pKa = 10.3 SNNFILDD479 pKa = 3.51 EE480 pKa = 4.45 FGKK483 pKa = 10.9 KK484 pKa = 9.61 IGDD487 pKa = 4.0 CNKK490 pKa = 8.67 NTLDD494 pKa = 3.03 IMTSIFEE501 pKa = 4.11 TRR503 pKa = 11.84 FWQCISDD510 pKa = 4.31 FSTLMKK516 pKa = 10.66 NILSVSQYY524 pKa = 10.08 NRR526 pKa = 11.84 HH527 pKa = 4.14 NTFRR531 pKa = 11.84 IAMCANNNLFALVYY545 pKa = 9.38 PSADD549 pKa = 2.75 IKK551 pKa = 9.6 TKK553 pKa = 10.35 KK554 pKa = 9.09 ATVVYY559 pKa = 10.24 SIIALHH565 pKa = 6.49 KK566 pKa = 11.0 NEE568 pKa = 4.34 TDD570 pKa = 3.6 VFNPGCLHH578 pKa = 6.59 GTFKK582 pKa = 11.13 CNNGYY587 pKa = 10.17 ISISRR592 pKa = 11.84 AIRR595 pKa = 11.84 LDD597 pKa = 3.19 KK598 pKa = 10.57 EE599 pKa = 3.82 RR600 pKa = 11.84 CQRR603 pKa = 11.84 IVSSPGLFLTTCLLFKK619 pKa = 10.76 HH620 pKa = 6.17 EE621 pKa = 4.46 NPTVRR626 pKa = 11.84 LEE628 pKa = 4.17 DD629 pKa = 3.34 VMTFSLFTSLSITKK643 pKa = 9.97 SVLSLTEE650 pKa = 3.56 PARR653 pKa = 11.84 YY654 pKa = 8.71 MIMNSLAISSNVKK667 pKa = 10.41 DD668 pKa = 4.0 YY669 pKa = 10.83 IAEE672 pKa = 4.2 KK673 pKa = 10.12 FSPYY677 pKa = 9.91 TKK679 pKa = 9.97 TLFSVYY685 pKa = 7.02 MTRR688 pKa = 11.84 LIKK691 pKa = 10.27 NACFEE696 pKa = 5.1 AYY698 pKa = 9.47 NQRR701 pKa = 11.84 QKK703 pKa = 10.91 VQLRR707 pKa = 11.84 DD708 pKa = 3.45 IYY710 pKa = 11.24 LSDD713 pKa = 3.8 YY714 pKa = 11.1 DD715 pKa = 3.83 ITQKK719 pKa = 10.85 GIKK722 pKa = 9.59 DD723 pKa = 3.59 NRR725 pKa = 11.84 EE726 pKa = 3.81 LTSIWFPGSVTLKK739 pKa = 10.5 EE740 pKa = 4.31 YY741 pKa = 9.32 LTQIYY746 pKa = 10.49 LPFYY750 pKa = 10.16 FNAKK754 pKa = 9.18 GLHH757 pKa = 5.79 EE758 pKa = 4.34 KK759 pKa = 9.64 HH760 pKa = 6.25 HH761 pKa = 6.35 VMVDD765 pKa = 3.53 LAKK768 pKa = 10.18 TILEE772 pKa = 4.19 IEE774 pKa = 3.99 NEE776 pKa = 3.95 QRR778 pKa = 11.84 NEE780 pKa = 3.49 ITKK783 pKa = 9.56 IWSKK787 pKa = 11.41 DD788 pKa = 3.31 LTKK791 pKa = 9.92 QTVNLQILIHH801 pKa = 5.69 SLCKK805 pKa = 10.39 NLLADD810 pKa = 4.26 TSRR813 pKa = 11.84 HH814 pKa = 3.59 NHH816 pKa = 4.57 LRR818 pKa = 11.84 NRR820 pKa = 11.84 IEE822 pKa = 3.6 NRR824 pKa = 11.84 NNFRR828 pKa = 11.84 RR829 pKa = 11.84 SITTISTFTSSKK841 pKa = 10.13 SCIKK845 pKa = 10.19 IGNFQKK851 pKa = 10.61 EE852 pKa = 4.24 KK853 pKa = 9.16 EE854 pKa = 4.3 VQYY857 pKa = 10.55 TRR859 pKa = 11.84 QRR861 pKa = 11.84 KK862 pKa = 8.95 NIEE865 pKa = 3.4 IEE867 pKa = 3.93 SRR869 pKa = 11.84 KK870 pKa = 9.66 RR871 pKa = 11.84 RR872 pKa = 11.84 LANPLFVSDD881 pKa = 4.37 EE882 pKa = 4.39 EE883 pKa = 4.3 IGLEE887 pKa = 4.56 VGHH890 pKa = 6.73 CNYY893 pKa = 11.0 NMLRR897 pKa = 11.84 EE898 pKa = 4.33 AMPEE902 pKa = 3.71 YY903 pKa = 10.17 RR904 pKa = 11.84 DD905 pKa = 3.87 YY906 pKa = 11.43 ISTKK910 pKa = 10.03 VFDD913 pKa = 4.74 RR914 pKa = 11.84 LYY916 pKa = 11.42 EE917 pKa = 4.04 MLDD920 pKa = 3.23 TDD922 pKa = 4.22 QVSDD926 pKa = 3.74 KK927 pKa = 9.99 PTIEE931 pKa = 5.76 LIMDD935 pKa = 3.74 MMADD939 pKa = 3.1 HH940 pKa = 7.27 TDD942 pKa = 3.6 FYY944 pKa = 11.31 FTFFNKK950 pKa = 9.41 GQKK953 pKa = 7.54 TSKK956 pKa = 10.36 DD957 pKa = 3.11 RR958 pKa = 11.84 EE959 pKa = 4.33 IFVGEE964 pKa = 4.43 YY965 pKa = 7.34 EE966 pKa = 5.49 AKK968 pKa = 7.92 MCMYY972 pKa = 10.38 AVEE975 pKa = 5.2 RR976 pKa = 11.84 IAKK979 pKa = 7.75 EE980 pKa = 3.85 RR981 pKa = 11.84 CKK983 pKa = 10.8 LNPDD987 pKa = 3.47 EE988 pKa = 5.2 MISEE992 pKa = 4.56 PGDD995 pKa = 3.45 GKK997 pKa = 11.32 LKK999 pKa = 10.64 VLEE1002 pKa = 4.2 QKK1004 pKa = 10.34 SEE1006 pKa = 3.82 QEE1008 pKa = 3.34 IRR1010 pKa = 11.84 FLVEE1014 pKa = 3.27 TTRR1017 pKa = 11.84 QKK1019 pKa = 10.96 NRR1021 pKa = 11.84 EE1022 pKa = 3.61 IDD1024 pKa = 3.58 EE1025 pKa = 5.05 AIEE1028 pKa = 3.73 TLAAEE1033 pKa = 4.17 NFEE1036 pKa = 4.56 GNISKK1041 pKa = 10.07 IEE1043 pKa = 3.88 KK1044 pKa = 8.94 LAKK1047 pKa = 10.1 GKK1049 pKa = 10.42 ARR1051 pKa = 11.84 GLKK1054 pKa = 9.21 MEE1056 pKa = 5.03 INADD1060 pKa = 3.45 MSKK1063 pKa = 10.21 WSAQDD1068 pKa = 3.37 VFFKK1072 pKa = 10.47 YY1073 pKa = 10.17 FWLIALDD1080 pKa = 4.48 PILYY1084 pKa = 8.62 PQEE1087 pKa = 3.83 KK1088 pKa = 9.6 EE1089 pKa = 3.77 RR1090 pKa = 11.84 IIFFFCNYY1098 pKa = 6.11 MQKK1101 pKa = 10.94 KK1102 pKa = 10.23 LILPDD1107 pKa = 5.08 DD1108 pKa = 4.44 LMCNLLDD1115 pKa = 3.98 QKK1117 pKa = 10.41 ATYY1120 pKa = 9.92 RR1121 pKa = 11.84 DD1122 pKa = 4.3 DD1123 pKa = 4.55 IISIMTNQLQTNCVEE1138 pKa = 4.82 IKK1140 pKa = 10.8 RR1141 pKa = 11.84 NWLQGNFNYY1150 pKa = 9.88 TSSYY1154 pKa = 8.74 VHH1156 pKa = 6.1 SCAMSVYY1163 pKa = 10.64 KK1164 pKa = 10.67 DD1165 pKa = 3.55 VLKK1168 pKa = 10.78 EE1169 pKa = 3.78 AMSYY1173 pKa = 11.07 LEE1175 pKa = 4.08 GSIVVNSLVHH1185 pKa = 7.05 SDD1187 pKa = 4.22 DD1188 pKa = 3.69 NQTSVTIVQDD1198 pKa = 3.56 KK1199 pKa = 11.22 APDD1202 pKa = 3.43 EE1203 pKa = 4.29 VLVEE1207 pKa = 4.41 FSIAQFQKK1215 pKa = 8.86 ICLAFGCQANMKK1227 pKa = 8.18 KK1228 pKa = 8.87 TYY1230 pKa = 8.44 VTNCIKK1236 pKa = 10.74 EE1237 pKa = 4.18 FVSLFNLYY1245 pKa = 10.55 GEE1247 pKa = 4.6 PFSIYY1252 pKa = 10.74 GRR1254 pKa = 11.84 FLLTSVGDD1262 pKa = 3.76 CAYY1265 pKa = 9.81 IGPYY1269 pKa = 9.66 EE1270 pKa = 4.41 DD1271 pKa = 3.25 LASRR1275 pKa = 11.84 ISSAQTAIKK1284 pKa = 9.85 HH1285 pKa = 5.37 GCPPSLAWVSIAISHH1300 pKa = 7.42 WITGLTYY1307 pKa = 11.42 NMLPGQSNDD1316 pKa = 3.01 PMDD1319 pKa = 4.12 YY1320 pKa = 10.73 FPAEE1324 pKa = 4.02 TRR1326 pKa = 11.84 KK1327 pKa = 10.62 DD1328 pKa = 3.25 IPIEE1332 pKa = 3.89 LNGVLDD1338 pKa = 4.78 APLSMISTVGLEE1350 pKa = 4.53 AGNLWFLIKK1359 pKa = 9.77 MLNKK1363 pKa = 8.69 HH1364 pKa = 5.33 TPIMQKK1370 pKa = 9.86 RR1371 pKa = 11.84 EE1372 pKa = 4.09 SVVNQISEE1380 pKa = 3.77 IRR1382 pKa = 11.84 NWDD1385 pKa = 3.99 LSRR1388 pKa = 11.84 MDD1390 pKa = 4.45 DD1391 pKa = 4.08 NEE1393 pKa = 3.87 IFKK1396 pKa = 11.0 LKK1398 pKa = 9.85 ILRR1401 pKa = 11.84 YY1402 pKa = 8.96 LVLDD1406 pKa = 3.98 AEE1408 pKa = 4.76 MDD1410 pKa = 3.72 PSDD1413 pKa = 4.38 IMGEE1417 pKa = 4.07 TSDD1420 pKa = 3.36 MRR1422 pKa = 11.84 GRR1424 pKa = 11.84 SILTPRR1430 pKa = 11.84 KK1431 pKa = 7.93 FTTSGSLRR1439 pKa = 11.84 KK1440 pKa = 9.63 LYY1442 pKa = 10.81 SFSKK1446 pKa = 9.47 YY1447 pKa = 7.17 QDD1449 pKa = 3.48 RR1450 pKa = 11.84 LSSPGGMNEE1459 pKa = 4.02 LFQYY1463 pKa = 11.01 LLEE1466 pKa = 5.06 KK1467 pKa = 10.81 PEE1469 pKa = 4.13 LLVTKK1474 pKa = 10.91 GEE1476 pKa = 4.16 DD1477 pKa = 3.0 KK1478 pKa = 10.61 KK1479 pKa = 11.29 DD1480 pKa = 3.61 YY1481 pKa = 8.99 MEE1483 pKa = 4.56 SVIFRR1488 pKa = 11.84 YY1489 pKa = 9.4 NSKK1492 pKa = 10.24 RR1493 pKa = 11.84 FKK1495 pKa = 10.7 EE1496 pKa = 4.16 SLSIQNPAQLFIEE1509 pKa = 4.55 QILFSNKK1516 pKa = 9.66 PIIDD1520 pKa = 3.9 FSGIRR1525 pKa = 11.84 DD1526 pKa = 3.68 KK1527 pKa = 11.39 YY1528 pKa = 11.02 INLHH1532 pKa = 6.46 DD1533 pKa = 3.88 SRR1535 pKa = 11.84 ALEE1538 pKa = 4.28 KK1539 pKa = 10.89 EE1540 pKa = 3.36 PDD1542 pKa = 3.59 IIGKK1546 pKa = 7.01 VTFRR1550 pKa = 11.84 EE1551 pKa = 4.45 AYY1553 pKa = 9.6 RR1554 pKa = 11.84 ILMKK1558 pKa = 10.58 DD1559 pKa = 3.71 LNSLPLTNDD1568 pKa = 4.48 DD1569 pKa = 3.42 IQVVYY1574 pKa = 10.57 SYY1576 pKa = 10.98 IILNDD1581 pKa = 3.7 PLMITIANTQILSIYY1596 pKa = 10.2 GSPQKK1601 pKa = 9.68 RR1602 pKa = 11.84 TGMSCSTMPEE1612 pKa = 3.72 FRR1614 pKa = 11.84 NLKK1617 pKa = 9.8 LIHH1620 pKa = 6.7 HH1621 pKa = 6.65 SPALVLRR1628 pKa = 11.84 AYY1630 pKa = 10.59 SKK1632 pKa = 9.01 NTPDD1636 pKa = 3.46 VQGADD1641 pKa = 3.68 PTEE1644 pKa = 3.84 MSRR1647 pKa = 11.84 DD1648 pKa = 3.51 LVHH1651 pKa = 7.2 LKK1653 pKa = 10.72 EE1654 pKa = 4.98 FVDD1657 pKa = 3.74 NTNLRR1662 pKa = 11.84 TKK1664 pKa = 8.91 MEE1666 pKa = 3.92 KK1667 pKa = 9.93 RR1668 pKa = 11.84 IMEE1671 pKa = 4.26 NEE1673 pKa = 4.04 LEE1675 pKa = 4.14 KK1676 pKa = 10.73 GARR1679 pKa = 11.84 DD1680 pKa = 3.14 IVFEE1684 pKa = 4.41 LKK1686 pKa = 10.66 EE1687 pKa = 3.81 MTRR1690 pKa = 11.84 FYY1692 pKa = 10.45 QVCYY1696 pKa = 9.96 EE1697 pKa = 4.2 YY1698 pKa = 10.57 IKK1700 pKa = 10.21 STEE1703 pKa = 3.87 HH1704 pKa = 6.47 KK1705 pKa = 9.28 IKK1707 pKa = 11.05 VFILPAKK1714 pKa = 10.3 AYY1716 pKa = 6.33 TTTDD1720 pKa = 3.53 FCSLMQGNLIKK1731 pKa = 10.82 DD1732 pKa = 3.82 KK1733 pKa = 10.53 EE1734 pKa = 4.67 WYY1736 pKa = 7.04 TVHH1739 pKa = 6.46 YY1740 pKa = 9.43 LKK1742 pKa = 10.77 QILSGGHH1749 pKa = 5.42 KK1750 pKa = 10.63 AIMQHH1755 pKa = 6.2 NSTSEE1760 pKa = 3.63 QNIAFEE1766 pKa = 4.38 CFRR1769 pKa = 11.84 LITHH1773 pKa = 7.11 FADD1776 pKa = 3.53 SFIDD1780 pKa = 3.61 AGSRR1784 pKa = 11.84 PAFLQLVLDD1793 pKa = 3.91 SFSYY1797 pKa = 11.19 KK1798 pKa = 10.07 DD1799 pKa = 3.37 VKK1801 pKa = 10.86 VNKK1804 pKa = 9.92 LYY1806 pKa = 10.72 EE1807 pKa = 4.38 IIKK1810 pKa = 9.88 NGHH1813 pKa = 5.86 NRR1815 pKa = 11.84 TDD1817 pKa = 4.58 FIPLLFRR1824 pKa = 11.84 TGDD1827 pKa = 3.42 LTQYY1831 pKa = 11.55 DD1832 pKa = 4.44 LDD1834 pKa = 5.3 KK1835 pKa = 10.9 YY1836 pKa = 11.3 DD1837 pKa = 3.57 AMKK1840 pKa = 10.53 SQEE1843 pKa = 3.95 RR1844 pKa = 11.84 VTWNDD1849 pKa = 2.76 WQTSRR1854 pKa = 11.84 HH1855 pKa = 5.67 LDD1857 pKa = 3.11 MGAINLTISGYY1868 pKa = 10.34 NRR1870 pKa = 11.84 AITIIGEE1877 pKa = 4.31 DD1878 pKa = 3.19 NKK1880 pKa = 10.23 LTYY1883 pKa = 10.23 SEE1885 pKa = 5.36 LNITRR1890 pKa = 11.84 KK1891 pKa = 7.7 TPEE1894 pKa = 4.39 NISISGRR1901 pKa = 11.84 KK1902 pKa = 8.96 LLSSRR1907 pKa = 11.84 HH1908 pKa = 4.46 GLKK1911 pKa = 10.3 FEE1913 pKa = 4.23 NMAKK1917 pKa = 8.65 VTTYY1921 pKa = 10.14 PGNYY1925 pKa = 8.78 YY1926 pKa = 8.32 ITYY1929 pKa = 9.23 RR1930 pKa = 11.84 KK1931 pKa = 9.35 KK1932 pKa = 10.47 DD1933 pKa = 3.24 RR1934 pKa = 11.84 HH1935 pKa = 4.91 QYY1937 pKa = 9.05 IYY1939 pKa = 10.25 QIHH1942 pKa = 4.62 THH1944 pKa = 5.36 EE1945 pKa = 5.32 SILRR1949 pKa = 11.84 RR1950 pKa = 11.84 NEE1952 pKa = 2.98 EE1953 pKa = 3.56 HH1954 pKa = 5.58 MAIRR1958 pKa = 11.84 TRR1960 pKa = 11.84 IFNEE1964 pKa = 3.73 IVPVCLVNVAEE1975 pKa = 4.48 IDD1977 pKa = 3.34 GDD1979 pKa = 3.69 QRR1981 pKa = 11.84 ILIRR1985 pKa = 11.84 KK1986 pKa = 9.08 LDD1988 pKa = 3.81 YY1989 pKa = 11.2 LNNDD1993 pKa = 2.86 IFSLARR1999 pKa = 11.84 IKK2001 pKa = 11.1 VGIDD2005 pKa = 2.96 EE2006 pKa = 4.32 YY2007 pKa = 11.79 AVIKK2011 pKa = 10.05 KK2012 pKa = 10.02 AHH2014 pKa = 5.95 FSKK2017 pKa = 10.22 MVSFDD2022 pKa = 3.58 GPPIKK2027 pKa = 9.82 TGLLNLTEE2035 pKa = 4.27 LMKK2038 pKa = 11.17 SQDD2041 pKa = 4.44 LLNLNYY2047 pKa = 10.99 DD2048 pKa = 4.0 NIRR2051 pKa = 11.84 NSNLISFSKK2060 pKa = 10.32 IICCEE2065 pKa = 3.73 GTDD2068 pKa = 4.33 NIDD2071 pKa = 3.25 EE2072 pKa = 4.44 GLEE2075 pKa = 3.97 FLSDD2079 pKa = 3.47 DD2080 pKa = 3.53 PMAFTEE2086 pKa = 4.61 GEE2088 pKa = 4.57 TIHH2091 pKa = 5.99 STPIFNIYY2099 pKa = 9.47 YY2100 pKa = 8.04 SKK2102 pKa = 10.65 KK2103 pKa = 9.59 GEE2105 pKa = 3.68 RR2106 pKa = 11.84 HH2107 pKa = 3.21 MTYY2110 pKa = 10.71 RR2111 pKa = 11.84 NAIKK2115 pKa = 10.51 LLIEE2119 pKa = 4.36 RR2120 pKa = 11.84 EE2121 pKa = 3.98 TSNFEE2126 pKa = 3.9 EE2127 pKa = 4.91 AFTFYY2132 pKa = 10.96 KK2133 pKa = 10.68 DD2134 pKa = 3.54 GFLSPEE2140 pKa = 3.78 NLGCLEE2146 pKa = 5.01 AIISLIRR2153 pKa = 11.84 VLKK2156 pKa = 7.88 TNEE2159 pKa = 3.35 WSTVIDD2165 pKa = 3.71 KK2166 pKa = 10.75 CIHH2169 pKa = 5.46 ICLIKK2174 pKa = 11.16 NNMDD2178 pKa = 3.64 YY2179 pKa = 10.78 LYY2181 pKa = 10.43 HH2182 pKa = 6.88 KK2183 pKa = 10.38 FDD2185 pKa = 4.12 MPSCFIDD2192 pKa = 4.6 DD2193 pKa = 4.87 PISRR2197 pKa = 11.84 NINWLMYY2204 pKa = 10.17 RR2205 pKa = 11.84 DD2206 pKa = 5.26 FISTLPPTYY2215 pKa = 9.77 IAPWNIMLEE2224 pKa = 4.15 HH2225 pKa = 6.57 FRR2227 pKa = 11.84 TKK2229 pKa = 10.5 CSTLIMDD2236 pKa = 4.73 KK2237 pKa = 10.51 MEE2239 pKa = 4.19 TQRR2242 pKa = 11.84 NFQDD2246 pKa = 3.54 FAKK2249 pKa = 9.81 MMRR2252 pKa = 11.84 RR2253 pKa = 11.84 SEE2255 pKa = 3.9 GRR2257 pKa = 11.84 TNLDD2261 pKa = 3.31 FSS2263 pKa = 4.24
Molecular weight: 263.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.29
IPC2_protein 6.389
IPC_protein 6.415
Toseland 6.561
ProMoST 6.839
Dawson 6.81
Bjellqvist 6.839
Wikipedia 6.81
Rodwell 6.81
Grimsley 6.664
Solomon 6.81
Lehninger 6.81
Nozaki 7.132
DTASelect 7.205
Thurlkill 7.249
EMBOSS 7.22
Sillero 7.22
Patrickios 4.228
IPC_peptide 6.825
IPC2_peptide 6.854
IPC2.peptide.svr19 6.813
Protein with the highest isoelectric point:
>tr|B0F1A8|B0F1A8_9VIRU Non-structural protein NS-S OS=Jamestown Canyon virus OX=35511 PE=4 SV=1
MM1 pKa = 7.78 GDD3 pKa = 3.42 LVFYY7 pKa = 10.86 DD8 pKa = 3.99 VASTGANGFDD18 pKa = 3.77 PDD20 pKa = 3.57 AGFVAFMADD29 pKa = 3.02 HH30 pKa = 6.73 GEE32 pKa = 4.34 SVSLSAVRR40 pKa = 11.84 IFFLNAAKK48 pKa = 10.46 AKK50 pKa = 10.23 AALARR55 pKa = 11.84 KK56 pKa = 8.7 PEE58 pKa = 4.16 RR59 pKa = 11.84 KK60 pKa = 8.36 ATPKK64 pKa = 9.97 FGEE67 pKa = 4.07 WQVEE71 pKa = 3.87 IVNNHH76 pKa = 5.7 FPGNRR81 pKa = 11.84 NNPIGNNDD89 pKa = 3.48 LTIHH93 pKa = 6.63 RR94 pKa = 11.84 LSGYY98 pKa = 7.92 LARR101 pKa = 11.84 WVLEE105 pKa = 4.07 HH106 pKa = 6.51 FTTDD110 pKa = 3.18 DD111 pKa = 4.26 DD112 pKa = 4.3 EE113 pKa = 4.89 SQRR116 pKa = 11.84 EE117 pKa = 4.39 LIRR120 pKa = 11.84 STIINPIAEE129 pKa = 4.44 SNGIHH134 pKa = 6.7 WNNGPEE140 pKa = 3.96 IYY142 pKa = 10.58 LSFFPGTEE150 pKa = 3.62 MFLEE154 pKa = 4.18 IFKK157 pKa = 10.31 FYY159 pKa = 10.43 PLTIGIYY166 pKa = 8.68 RR167 pKa = 11.84 VKK169 pKa = 10.29 HH170 pKa = 5.41 GMMDD174 pKa = 3.52 PQYY177 pKa = 10.7 LKK179 pKa = 10.33 KK180 pKa = 10.47 ALRR183 pKa = 11.84 QRR185 pKa = 11.84 YY186 pKa = 6.78 GTLTAEE192 pKa = 3.78 KK193 pKa = 9.83 WMAQKK198 pKa = 9.33 TVLIAKK204 pKa = 8.39 SLKK207 pKa = 10.5 DD208 pKa = 3.56 VEE210 pKa = 4.15 QLKK213 pKa = 9.69 WGRR216 pKa = 11.84 GGLSDD221 pKa = 4.12 AARR224 pKa = 11.84 TFLIKK229 pKa = 10.61 FGVKK233 pKa = 10.39 LPP235 pKa = 3.84
Molecular weight: 26.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.652
IPC2_protein 8.565
IPC_protein 8.536
Toseland 9.268
ProMoST 9.019
Dawson 9.531
Bjellqvist 9.18
Wikipedia 9.692
Rodwell 9.794
Grimsley 9.604
Solomon 9.604
Lehninger 9.56
Nozaki 9.209
DTASelect 9.18
Thurlkill 9.355
EMBOSS 9.677
Sillero 9.428
Patrickios 5.08
IPC_peptide 9.589
IPC2_peptide 7.658
IPC2.peptide.svr19 7.649
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
4034
92
2263
1008.5
116.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.859 ± 0.685
2.776 ± 1.028
5.9 ± 0.483
6.123 ± 0.485
4.784 ± 0.4
4.437 ± 0.822
2.429 ± 0.173
8.775 ± 0.396
7.66 ± 0.345
9.073 ± 0.963
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.876 ± 0.506
5.602 ± 0.564
3.198 ± 0.281
3.446 ± 0.464
4.462 ± 0.465
6.842 ± 0.484
6.296 ± 0.412
4.809 ± 0.619
1.215 ± 0.267
4.437 ± 0.557
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here