Feline cyclovirus
Average proteome isoelectric point is 8.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076JYW4|A0A076JYW4_9CIRC Capsid protein OS=Feline cyclovirus OX=1530454 PE=4 SV=1
MM1 pKa = 7.28 MMTIKK6 pKa = 10.44 NYY8 pKa = 8.38 KK9 pKa = 5.76 TTHH12 pKa = 6.57 EE13 pKa = 4.36 IGANTPLLEE22 pKa = 4.91 KK23 pKa = 9.99 KK24 pKa = 8.42 TLQQQEE30 pKa = 4.03 HH31 pKa = 5.73 HH32 pKa = 5.98 TFKK35 pKa = 10.52 DD36 pKa = 3.2 TCKK39 pKa = 10.84 YY40 pKa = 10.61 NINNLFLYY48 pKa = 10.3 RR49 pKa = 11.84 HH50 pKa = 6.47 FSTSKK55 pKa = 10.17 LFKK58 pKa = 9.99 TIKK61 pKa = 9.77 KK62 pKa = 9.3 QLGNEE67 pKa = 3.6 IHH69 pKa = 7.12 IEE71 pKa = 3.75 IARR74 pKa = 11.84 GDD76 pKa = 3.68 DD77 pKa = 3.79 YY78 pKa = 11.85 QNKK81 pKa = 9.93 DD82 pKa = 3.38 YY83 pKa = 9.69 CTKK86 pKa = 9.81 TDD88 pKa = 3.35 KK89 pKa = 10.95 EE90 pKa = 4.03 AWTWGVPIKK99 pKa = 10.46 QGQCTDD105 pKa = 3.49 LKK107 pKa = 10.38 RR108 pKa = 11.84 ACKK111 pKa = 9.9 AIKK114 pKa = 9.42 EE115 pKa = 4.42 GKK117 pKa = 8.3 SMKK120 pKa = 10.03 YY121 pKa = 9.24 ICEE124 pKa = 4.17 NFDD127 pKa = 4.58 DD128 pKa = 4.28 IYY130 pKa = 10.45 VRR132 pKa = 11.84 YY133 pKa = 8.91 WRR135 pKa = 11.84 GLYY138 pKa = 9.64 KK139 pKa = 10.6 LKK141 pKa = 9.6 EE142 pKa = 4.25 TLAPPPEE149 pKa = 4.4 RR150 pKa = 11.84 DD151 pKa = 3.52 FPTEE155 pKa = 3.41 VYY157 pKa = 10.28 YY158 pKa = 10.87 YY159 pKa = 10.02 YY160 pKa = 11.07 GEE162 pKa = 4.47 PGVGKK167 pKa = 9.68 SRR169 pKa = 11.84 RR170 pKa = 11.84 AYY172 pKa = 10.74 SEE174 pKa = 4.37 AKK176 pKa = 8.7 ATNEE180 pKa = 3.86 SIYY183 pKa = 10.47 YY184 pKa = 9.72 KK185 pKa = 10.52 PRR187 pKa = 11.84 GDD189 pKa = 2.66 WWDD192 pKa = 3.86 GYY194 pKa = 7.82 EE195 pKa = 3.93 QQPNVIIDD203 pKa = 4.32 DD204 pKa = 4.42 FYY206 pKa = 11.73 GWIKK210 pKa = 10.75 YY211 pKa = 10.28 DD212 pKa = 3.83 EE213 pKa = 4.69 LLKK216 pKa = 11.02 IMDD219 pKa = 4.75 RR220 pKa = 11.84 YY221 pKa = 9.64 PYY223 pKa = 9.85 RR224 pKa = 11.84 VPVKK228 pKa = 10.39 GGYY231 pKa = 9.78 QIFNSKK237 pKa = 10.34 RR238 pKa = 11.84 IWITSNIPIDD248 pKa = 3.58 QLYY251 pKa = 9.4 KK252 pKa = 9.99 HH253 pKa = 6.23 SHH255 pKa = 7.53 FIPQAIEE262 pKa = 3.85 RR263 pKa = 11.84 RR264 pKa = 11.84 CTKK267 pKa = 10.03 IEE269 pKa = 3.84 YY270 pKa = 8.2 MQQ272 pKa = 4.18
Molecular weight: 32.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.456
IPC2_protein 8.448
IPC_protein 8.317
Toseland 8.756
ProMoST 8.77
Dawson 9.165
Bjellqvist 9.077
Wikipedia 9.341
Rodwell 9.282
Grimsley 9.18
Solomon 9.224
Lehninger 9.18
Nozaki 9.107
DTASelect 8.975
Thurlkill 9.048
EMBOSS 9.282
Sillero 9.224
Patrickios 4.075
IPC_peptide 9.209
IPC2_peptide 7.79
IPC2.peptide.svr19 7.688
Protein with the highest isoelectric point:
>tr|A0A076JYW4|A0A076JYW4_9CIRC Capsid protein OS=Feline cyclovirus OX=1530454 PE=4 SV=1
MM1 pKa = 7.94 RR2 pKa = 11.84 SRR4 pKa = 11.84 GVRR7 pKa = 11.84 MRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 FFVRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 YY18 pKa = 10.05 RR19 pKa = 11.84 KK20 pKa = 9.01 FFRR23 pKa = 11.84 PGYY26 pKa = 7.92 RR27 pKa = 11.84 ALFRR31 pKa = 11.84 RR32 pKa = 11.84 YY33 pKa = 8.03 YY34 pKa = 9.44 RR35 pKa = 11.84 PYY37 pKa = 9.83 VRR39 pKa = 11.84 RR40 pKa = 11.84 PHH42 pKa = 6.52 NSALNTVLLRR52 pKa = 11.84 PNWTDD57 pKa = 3.25 NILPTVAKK65 pKa = 9.37 TRR67 pKa = 11.84 HH68 pKa = 5.53 LLSFKK73 pKa = 10.67 FDD75 pKa = 4.37 DD76 pKa = 4.47 LPEE79 pKa = 4.51 LEE81 pKa = 4.61 KK82 pKa = 10.97 FKK84 pKa = 11.36 SLFGYY89 pKa = 9.51 YY90 pKa = 9.71 RR91 pKa = 11.84 FVRR94 pKa = 11.84 FYY96 pKa = 9.25 TKK98 pKa = 10.37 CIPKK102 pKa = 8.49 VTVLQDD108 pKa = 3.32 NTSSGTYY115 pKa = 9.54 VAVPWHH121 pKa = 6.77 RR122 pKa = 11.84 YY123 pKa = 7.72 IDD125 pKa = 3.69 EE126 pKa = 4.65 ASLEE130 pKa = 4.38 KK131 pKa = 10.63 INADD135 pKa = 3.89 DD136 pKa = 5.43 LLDD139 pKa = 3.73 LTRR142 pKa = 11.84 SKK144 pKa = 11.06 ARR146 pKa = 11.84 RR147 pKa = 11.84 CNQVMYY153 pKa = 10.39 NSVVPAVVTNSNVLPNSTMRR173 pKa = 11.84 TITYY177 pKa = 9.73 RR178 pKa = 11.84 PWIKK182 pKa = 9.53 IDD184 pKa = 3.58 RR185 pKa = 11.84 DD186 pKa = 3.53 GTAVQHH192 pKa = 6.66 FGLLYY197 pKa = 10.51 AFPSDD202 pKa = 3.53 SLHH205 pKa = 5.54 ITFQIVTRR213 pKa = 11.84 CYY215 pKa = 8.81 VQLRR219 pKa = 11.84 GYY221 pKa = 10.05 SVV223 pKa = 2.89
Molecular weight: 26.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 10.043
IPC_protein 10.906
Toseland 10.643
ProMoST 10.511
Dawson 10.804
Bjellqvist 10.599
Wikipedia 11.082
Rodwell 10.891
Grimsley 10.891
Solomon 10.921
Lehninger 10.862
Nozaki 10.628
DTASelect 10.584
Thurlkill 10.672
EMBOSS 11.052
Sillero 10.73
Patrickios 10.511
IPC_peptide 10.921
IPC2_peptide 9.721
IPC2.peptide.svr19 8.147
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
495
223
272
247.5
29.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.04 ± 0.308
1.818 ± 0.328
5.455 ± 0.362
4.444 ± 1.838
4.646 ± 1.131
4.444 ± 0.905
2.424 ± 0.126
6.667 ± 1.824
8.081 ± 2.494
6.869 ± 1.456
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.02 ± 0.157
4.646 ± 0.198
5.051 ± 0.229
3.636 ± 0.967
8.889 ± 3.165
4.646 ± 1.131
6.869 ± 0.212
5.455 ± 2.437
2.02 ± 0.468
7.879 ± 0.799
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here