Chicken stool-associated circular virus
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L6KW42|A0A1L6KW42_9VIRU Replication associated protein OS=Chicken stool-associated circular virus OX=1930302 PE=3 SV=1
MM1 pKa = 7.44 SWNKK5 pKa = 7.13 YY6 pKa = 6.24 TKK8 pKa = 9.96 PLFLTCEE15 pKa = 5.09 DD16 pKa = 3.39 ITSGPRR22 pKa = 11.84 VWIPPRR28 pKa = 11.84 GMAYY32 pKa = 10.28 SRR34 pKa = 11.84 VILMSNGTDD43 pKa = 4.42 RR44 pKa = 11.84 PYY46 pKa = 10.68 PWYY49 pKa = 10.01 DD50 pKa = 2.94 NKK52 pKa = 10.94 LYY54 pKa = 10.29 EE55 pKa = 4.52 IKK57 pKa = 10.74 DD58 pKa = 3.61 EE59 pKa = 4.52 GEE61 pKa = 4.03 HH62 pKa = 5.93 VLLEE66 pKa = 4.34 RR67 pKa = 11.84 VDD69 pKa = 3.56 EE70 pKa = 4.54 GRR72 pKa = 11.84 PIMLYY77 pKa = 9.57 RR78 pKa = 11.84 PRR80 pKa = 11.84 VIIDD84 pKa = 3.38 TSKK87 pKa = 10.55 HH88 pKa = 4.34 YY89 pKa = 9.95 QTMEE93 pKa = 3.92 KK94 pKa = 9.57 TNKK97 pKa = 8.24 TVYY100 pKa = 10.42 PLVEE104 pKa = 4.45 EE105 pKa = 4.41 YY106 pKa = 11.15 NLLGTVPKK114 pKa = 10.43 TNVTITNDD122 pKa = 3.01 IKK124 pKa = 11.11 DD125 pKa = 4.26 EE126 pKa = 4.08 KK127 pKa = 10.56 WPDD130 pKa = 3.64 EE131 pKa = 5.13 DD132 pKa = 4.94 PDD134 pKa = 4.08 NPDD137 pKa = 3.5 EE138 pKa = 4.51 INRR141 pKa = 11.84 DD142 pKa = 3.8 FPPYY146 pKa = 10.07 GYY148 pKa = 10.8 SKK150 pKa = 9.84 VTTEE154 pKa = 4.18 IKK156 pKa = 10.14 WNGEE160 pKa = 3.79 DD161 pKa = 5.98 FPIHH165 pKa = 5.82 WLEE168 pKa = 3.8 QKK170 pKa = 10.16 EE171 pKa = 4.16 YY172 pKa = 10.11 NLEE175 pKa = 4.15 KK176 pKa = 10.34 EE177 pKa = 4.09 TDD179 pKa = 3.62 EE180 pKa = 4.43 NGITIDD186 pKa = 3.33 EE187 pKa = 4.39 WWRR190 pKa = 11.84 EE191 pKa = 4.03 TAIEE195 pKa = 4.26 GADD198 pKa = 3.4 NIYY201 pKa = 10.97 YY202 pKa = 10.46 CGLKK206 pKa = 9.29 MNVVIRR212 pKa = 11.84 ISVSAINRR220 pKa = 11.84 EE221 pKa = 4.04 SLDD224 pKa = 3.42 TFKK227 pKa = 11.12 FSLAGGEE234 pKa = 3.84 ILQPGEE240 pKa = 3.92 SGIFIQRR247 pKa = 11.84 TLDD250 pKa = 3.4 VDD252 pKa = 4.18 SSPVEE257 pKa = 3.9 YY258 pKa = 10.64 QITLVYY264 pKa = 11.15 NNTNEE269 pKa = 3.82 IKK271 pKa = 9.78 TVFKK275 pKa = 10.39 PVSQTRR281 pKa = 11.84 HH282 pKa = 5.39 ALLDD286 pKa = 3.81 FSDD289 pKa = 4.47 GYY291 pKa = 10.53 KK292 pKa = 10.13 IKK294 pKa = 10.53 IKK296 pKa = 10.58 AGHH299 pKa = 6.91 DD300 pKa = 2.98 KK301 pKa = 11.41 DD302 pKa = 3.8 MIEE305 pKa = 3.97 IQGDD309 pKa = 3.85 VTTLNIGSGLPEE321 pKa = 3.97 ITCSLSAKK329 pKa = 7.71 MFSMIFGNNWKK340 pKa = 9.73 PDD342 pKa = 3.62 DD343 pKa = 3.88 EE344 pKa = 4.46
Molecular weight: 39.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.713
IPC2_protein 4.724
IPC_protein 4.66
Toseland 4.52
ProMoST 4.736
Dawson 4.609
Bjellqvist 4.774
Wikipedia 4.482
Rodwell 4.507
Grimsley 4.431
Solomon 4.609
Lehninger 4.571
Nozaki 4.724
DTASelect 4.889
Thurlkill 4.52
EMBOSS 4.495
Sillero 4.787
Patrickios 3.884
IPC_peptide 4.622
IPC2_peptide 4.774
IPC2.peptide.svr19 4.762
Protein with the highest isoelectric point:
>tr|A0A1L6KW76|A0A1L6KW76_9VIRU Capsid protein OS=Chicken stool-associated circular virus OX=1930302 PE=4 SV=1
MM1 pKa = 7.9 AYY3 pKa = 10.02 ASSYY7 pKa = 10.44 FRR9 pKa = 11.84 TRR11 pKa = 11.84 YY12 pKa = 9.45 ASYY15 pKa = 10.45 RR16 pKa = 11.84 RR17 pKa = 11.84 AARR20 pKa = 11.84 GTRR23 pKa = 11.84 RR24 pKa = 11.84 WNSGSRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 AWGNARR38 pKa = 11.84 AARR41 pKa = 11.84 QQRR44 pKa = 11.84 DD45 pKa = 3.25 ACTVTISRR53 pKa = 11.84 LTNVSVPVAASQTSGTAFIAHH74 pKa = 7.24 WDD76 pKa = 3.63 SLRR79 pKa = 11.84 QSTYY83 pKa = 9.36 FPNYY87 pKa = 9.79 SSMYY91 pKa = 10.05 DD92 pKa = 3.12 QIKK95 pKa = 9.5 IDD97 pKa = 3.88 KK98 pKa = 10.19 IRR100 pKa = 11.84 VKK102 pKa = 10.11 VTGTNAGASTDD113 pKa = 3.9 LVSPTVVLAFDD124 pKa = 4.53 RR125 pKa = 11.84 NGLDD129 pKa = 3.41 SAQLNSDD136 pKa = 4.71 GISLATGVQGVSTYY150 pKa = 11.22 SSAQSRR156 pKa = 11.84 QWSVGNAFTMWQTIYY171 pKa = 10.49 PSTIMEE177 pKa = 4.44 KK178 pKa = 10.52 GQYY181 pKa = 9.73 IPTNSLSTSSTTANPAYY198 pKa = 10.19 VISDD202 pKa = 3.12 STMPFKK208 pKa = 10.63 PVTLFGISTNGTEE221 pKa = 4.19 VTSAQTFVFSVEE233 pKa = 3.88 LEE235 pKa = 4.12 YY236 pKa = 10.73 TVTFRR241 pKa = 11.84 GMRR244 pKa = 11.84 KK245 pKa = 9.53 PEE247 pKa = 3.83 IQTGSS252 pKa = 3.16
Molecular weight: 27.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.652
IPC2_protein 9.765
IPC_protein 10.452
Toseland 9.97
ProMoST 9.926
Dawson 10.292
Bjellqvist 10.087
Wikipedia 10.555
Rodwell 10.394
Grimsley 10.423
Solomon 10.335
Lehninger 10.277
Nozaki 9.97
DTASelect 10.072
Thurlkill 10.101
EMBOSS 10.423
Sillero 10.204
Patrickios 5.347
IPC_peptide 10.321
IPC2_peptide 9.063
IPC2.peptide.svr19 8.619
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
923
252
344
307.7
35.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.309 ± 1.628
1.733 ± 0.899
6.717 ± 1.057
6.392 ± 1.531
3.467 ± 0.271
6.717 ± 0.447
1.842 ± 0.72
6.501 ± 1.214
6.826 ± 1.725
6.067 ± 0.711
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.925 ± 0.345
4.875 ± 0.618
4.117 ± 1.036
3.359 ± 0.71
5.309 ± 0.895
6.717 ± 2.401
7.909 ± 1.493
6.067 ± 0.37
2.709 ± 0.32
4.442 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here