Paludifilum halophilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Paludifilum

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A235BCR6|A0A235BCR6_9BACL Riboflavin biosynthesis protein RibD OS=Paludifilum halophilum OX=1642702 GN=ribD PE=3 SV=1
MM1 pKa = 7.2RR2 pKa = 11.84TWVDD6 pKa = 2.81KK7 pKa = 9.29DD8 pKa = 3.37TCIACGACGAAAPDD22 pKa = 3.73VYY24 pKa = 11.0DD25 pKa = 3.88YY26 pKa = 11.72DD27 pKa = 3.82EE28 pKa = 5.92DD29 pKa = 6.11GIAFVLLDD37 pKa = 4.93DD38 pKa = 4.16NTGTATVPEE47 pKa = 4.45EE48 pKa = 3.96FHH50 pKa = 7.78DD51 pKa = 4.55DD52 pKa = 3.55VLDD55 pKa = 4.34ARR57 pKa = 11.84DD58 pKa = 3.91GCPTDD63 pKa = 3.69SIKK66 pKa = 10.82VEE68 pKa = 4.13EE69 pKa = 4.22

Molecular weight: 7.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A235B6M1|A0A235B6M1_9BACL Uncharacterized protein OS=Paludifilum halophilum OX=1642702 GN=CHM34_07395 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.86RR12 pKa = 11.84KK13 pKa = 8.37NVHH16 pKa = 5.44GFRR19 pKa = 11.84QRR21 pKa = 11.84MSTKK25 pKa = 10.02NGRR28 pKa = 11.84KK29 pKa = 8.03VLRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.97GRR39 pKa = 11.84KK40 pKa = 8.63ILSAA44 pKa = 4.02

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3615

0

3615

1049894

28

3168

290.4

32.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.566 ± 0.051

0.803 ± 0.012

5.368 ± 0.038

7.609 ± 0.049

3.945 ± 0.03

7.869 ± 0.037

2.216 ± 0.022

5.91 ± 0.036

5.279 ± 0.04

9.839 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.686 ± 0.02

3.111 ± 0.025

4.533 ± 0.03

3.74 ± 0.026

6.659 ± 0.039

5.788 ± 0.028

5.115 ± 0.025

7.496 ± 0.032

1.402 ± 0.018

3.068 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski