CRESS virus sp. ctfsu28
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W9N6|A0A5Q2W9N6_9VIRU ATP-dependent helicase Rep OS=CRESS virus sp. ctfsu28 OX=2656685 PE=3 SV=1
MM1 pKa = 7.0 AHH3 pKa = 7.3 KK4 pKa = 10.23 RR5 pKa = 11.84 KK6 pKa = 9.16 QWFYY10 pKa = 10.19 GSNSVRR16 pKa = 11.84 RR17 pKa = 11.84 SVRR20 pKa = 11.84 QQEE23 pKa = 4.33 DD24 pKa = 4.24 GNTPEE29 pKa = 5.17 LKK31 pKa = 10.08 MYY33 pKa = 9.7 DD34 pKa = 3.59 QSVLDD39 pKa = 3.95 TLIGTGTSWSGADD52 pKa = 4.01 MDD54 pKa = 4.97 PSSNTLFSPNLGVDD68 pKa = 3.41 MGQRR72 pKa = 11.84 IGRR75 pKa = 11.84 RR76 pKa = 11.84 CAVVGLDD83 pKa = 3.16 MNGFIDD89 pKa = 5.79 CNDD92 pKa = 2.98 QVLAVGADD100 pKa = 3.47 PAAICRR106 pKa = 11.84 IIVFQDD112 pKa = 3.26 TQTKK116 pKa = 9.83 GVQALGNLVMEE127 pKa = 5.12 AQTADD132 pKa = 3.36 AFHH135 pKa = 7.22 VITAFPSRR143 pKa = 11.84 DD144 pKa = 3.36 AIGRR148 pKa = 11.84 FIILKK153 pKa = 10.4 DD154 pKa = 3.4 MVYY157 pKa = 11.17 VMQNPAFSSLGSSGVTYY174 pKa = 9.98 DD175 pKa = 3.25 QQGIAKK181 pKa = 8.49 WFSFRR186 pKa = 11.84 IEE188 pKa = 3.7 FDD190 pKa = 3.27 EE191 pKa = 4.08 PVIVHH196 pKa = 6.47 FDD198 pKa = 3.21 ASDD201 pKa = 3.24 AGTVADD207 pKa = 3.75 VLDD210 pKa = 3.97 NSFHH214 pKa = 7.34 VMASNSSSGLSPRR227 pKa = 11.84 LTYY230 pKa = 7.32 TTRR233 pKa = 11.84 VYY235 pKa = 11.05 YY236 pKa = 10.81 YY237 pKa = 10.8 DD238 pKa = 3.51 FF239 pKa = 5.41
Molecular weight: 26.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.82
IPC2_protein 4.876
IPC_protein 4.825
Toseland 4.673
ProMoST 5.003
Dawson 4.851
Bjellqvist 4.978
Wikipedia 4.8
Rodwell 4.698
Grimsley 4.596
Solomon 4.838
Lehninger 4.8
Nozaki 4.965
DTASelect 5.245
Thurlkill 4.724
EMBOSS 4.825
Sillero 4.991
Patrickios 3.897
IPC_peptide 4.838
IPC2_peptide 4.978
IPC2.peptide.svr19 4.961
Protein with the highest isoelectric point:
>tr|A0A5Q2WAC3|A0A5Q2WAC3_9VIRU Capsid protein OS=CRESS virus sp. ctfsu28 OX=2656685 PE=4 SV=1
MM1 pKa = 7.48 AGKK4 pKa = 10.01 RR5 pKa = 11.84 KK6 pKa = 10.11 YY7 pKa = 10.03 KK8 pKa = 10.48 ADD10 pKa = 3.72 YY11 pKa = 9.73 KK12 pKa = 11.09 KK13 pKa = 10.91 DD14 pKa = 3.2 GAGVKK19 pKa = 9.62 KK20 pKa = 10.73 YY21 pKa = 10.16 KK22 pKa = 10.76 GSASQQNYY30 pKa = 8.3 IKK32 pKa = 10.48 RR33 pKa = 11.84 EE34 pKa = 4.02 YY35 pKa = 10.47 VPRR38 pKa = 11.84 TPGGAVVSEE47 pKa = 4.38 LKK49 pKa = 11.03 VIDD52 pKa = 3.97 SFLANTVLSAPTSWANTEE70 pKa = 3.93 VDD72 pKa = 4.09 PATLNCLFAPGPGTGLNQRR91 pKa = 11.84 IGRR94 pKa = 11.84 KK95 pKa = 9.08 VAVKK99 pKa = 9.65 KK100 pKa = 10.78 LKK102 pKa = 10.19 VRR104 pKa = 11.84 GLITCASQVDD114 pKa = 4.04 QTTPDD119 pKa = 3.05 NGCQVRR125 pKa = 11.84 LIFYY129 pKa = 10.65 QDD131 pKa = 3.16 MQTNGAQAQGEE142 pKa = 4.54 DD143 pKa = 4.87 LMDD146 pKa = 3.73 TPTASVQGVFNSFQNTASFGRR167 pKa = 11.84 FRR169 pKa = 11.84 VLKK172 pKa = 10.4 DD173 pKa = 2.89 MCIEE177 pKa = 3.94 INNPNISWDD186 pKa = 3.69 GTNLEE191 pKa = 3.87 QQGIVKK197 pKa = 10.11 RR198 pKa = 11.84 FKK200 pKa = 9.6 FSKK203 pKa = 9.76 VFKK206 pKa = 9.63 EE207 pKa = 4.04 PVVVHH212 pKa = 5.83 YY213 pKa = 10.25 NATGGTTVADD223 pKa = 3.62 VVDD226 pKa = 4.15 NSFHH230 pKa = 7.11 AIAVVNSAALVPNLSYY246 pKa = 10.94 VCRR249 pKa = 11.84 VTYY252 pKa = 10.55 TDD254 pKa = 3.1 VV255 pKa = 3.0
Molecular weight: 27.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.73
IPC2_protein 8.609
IPC_protein 8.507
Toseland 9.165
ProMoST 9.004
Dawson 9.472
Bjellqvist 9.297
Wikipedia 9.619
Rodwell 9.706
Grimsley 9.502
Solomon 9.531
Lehninger 9.502
Nozaki 9.443
DTASelect 9.209
Thurlkill 9.341
EMBOSS 9.619
Sillero 9.487
Patrickios 4.736
IPC_peptide 9.531
IPC2_peptide 8.009
IPC2.peptide.svr19 7.76
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
816
239
322
272.0
30.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.985 ± 0.962
2.328 ± 0.538
7.23 ± 0.742
4.412 ± 1.444
4.044 ± 0.816
8.088 ± 0.142
1.593 ± 0.336
5.025 ± 0.429
6.618 ± 1.585
5.882 ± 0.175
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.574 ± 0.492
5.392 ± 0.556
3.676 ± 0.247
3.676 ± 1.295
5.27 ± 0.387
6.373 ± 1.103
5.515 ± 0.912
8.701 ± 1.173
1.961 ± 0.693
4.657 ± 0.881
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here