Acinetobacter bouvetii DSM 14964 = CIP 107468
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3110 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|N9DSD3|N9DSD3_9GAMM Probable proton/glutamate-aspartate symporter OS=Acinetobacter bouvetii DSM 14964 = CIP 107468 OX=1120925 GN=gltP PE=3 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.11 KK3 pKa = 9.69 YY4 pKa = 10.65 QCIVCGWIYY13 pKa = 11.09 DD14 pKa = 4.04 EE15 pKa = 5.55 AEE17 pKa = 3.9 GWPQDD22 pKa = 3.98 GIVAGTKK29 pKa = 9.37 WDD31 pKa = 5.55 DD32 pKa = 4.36 IPDD35 pKa = 3.76 DD36 pKa = 4.19 WTCPDD41 pKa = 4.33 CGVSKK46 pKa = 11.16 ADD48 pKa = 3.61 FEE50 pKa = 4.53 MVEE53 pKa = 4.0 II54 pKa = 4.86
Molecular weight: 6.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|N9DFE2|N9DFE2_9GAMM MFS domain-containing protein OS=Acinetobacter bouvetii DSM 14964 = CIP 107468 OX=1120925 GN=F941_03194 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3110
0
3110
955441
23
3078
307.2
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.623 ± 0.051
0.969 ± 0.014
5.125 ± 0.037
5.738 ± 0.048
4.293 ± 0.039
6.673 ± 0.056
2.366 ± 0.029
6.63 ± 0.037
5.466 ± 0.038
10.436 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.644 ± 0.028
4.321 ± 0.043
3.996 ± 0.027
5.382 ± 0.051
4.232 ± 0.039
6.225 ± 0.042
4.949 ± 0.039
6.506 ± 0.039
1.257 ± 0.019
3.169 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here