Arthrobacter sp. Edens01
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3212 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N8HX36|A0A0N8HX36_9MICC Uncharacterized protein OS=Arthrobacter sp. Edens01 OX=1732020 GN=AO716_16090 PE=3 SV=1
MM1 pKa = 6.85 TRR3 pKa = 11.84 ALIVVDD9 pKa = 3.88 VQNDD13 pKa = 3.59 FCEE16 pKa = 4.95 GGSLAVPGGNDD27 pKa = 3.02 TAAEE31 pKa = 3.81 ISDD34 pKa = 4.26 YY35 pKa = 11.21 IEE37 pKa = 4.01 TSRR40 pKa = 11.84 DD41 pKa = 2.95 RR42 pKa = 11.84 YY43 pKa = 10.61 AVVAATQDD51 pKa = 3.1 WHH53 pKa = 7.42 IEE55 pKa = 4.13 PGSHH59 pKa = 7.5 FSDD62 pKa = 3.25 NPNFRR67 pKa = 11.84 DD68 pKa = 3.44 SWPVHH73 pKa = 5.95 CVAGTHH79 pKa = 6.15 GAALNPDD86 pKa = 4.32 LDD88 pKa = 4.05 TEE90 pKa = 4.82 FIDD93 pKa = 4.35 AYY95 pKa = 10.2 FRR97 pKa = 11.84 KK98 pKa = 9.54 GQYY101 pKa = 8.2 EE102 pKa = 3.74 AAYY105 pKa = 10.48 SGFEE109 pKa = 4.04 GLLAPDD115 pKa = 3.74 VEE117 pKa = 4.88 VPLGDD122 pKa = 4.26 PDD124 pKa = 5.11 AEE126 pKa = 4.3 PEE128 pKa = 4.02 PDD130 pKa = 3.53 EE131 pKa = 5.48 DD132 pKa = 5.71 AVSLDD137 pKa = 3.07 DD138 pKa = 3.68 WLRR141 pKa = 11.84 DD142 pKa = 3.58 NGVDD146 pKa = 3.34 EE147 pKa = 4.33 VVIVGLATDD156 pKa = 3.84 YY157 pKa = 10.85 CVKK160 pKa = 10.11 ATALDD165 pKa = 3.86 AVAAGYY171 pKa = 7.79 ATYY174 pKa = 10.5 VIPEE178 pKa = 4.64 LCRR181 pKa = 11.84 GLDD184 pKa = 3.53 KK185 pKa = 11.37 VDD187 pKa = 3.45 VLAALDD193 pKa = 3.9 EE194 pKa = 4.8 LEE196 pKa = 4.48 DD197 pKa = 4.47 AGVEE201 pKa = 4.39 LLDD204 pKa = 4.28 LL205 pKa = 4.67
Molecular weight: 22.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.706
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.846
Patrickios 1.163
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A0P7G887|A0A0P7G887_9MICC Uncharacterized protein OS=Arthrobacter sp. Edens01 OX=1732020 GN=AO716_08540 PE=4 SV=1
MM1 pKa = 7.28 TGIAIACALASAVFLAFGAQRR22 pKa = 11.84 QGSAVSADD30 pKa = 3.4 TGGLSLNSAGLGRR43 pKa = 11.84 LLRR46 pKa = 11.84 NPRR49 pKa = 11.84 WLFGLLLLGVGTGLNVAALAMAPLTVVQPIGSLALVITTIVNSRR93 pKa = 11.84 DD94 pKa = 2.87 QGLRR98 pKa = 11.84 LNRR101 pKa = 11.84 VTVVSIVACVLGSMLFVLLAVGATRR126 pKa = 11.84 SEE128 pKa = 4.27 PVVEE132 pKa = 4.05 ARR134 pKa = 11.84 QEE136 pKa = 3.92 IVIVLILAVVVAFFGMLNLVFGKK159 pKa = 10.41 RR160 pKa = 11.84 LGAIAHH166 pKa = 6.48 ILGAGILFGFVAVLTKK182 pKa = 10.19 TIAADD187 pKa = 3.66 LLDD190 pKa = 4.26 PNGRR194 pKa = 11.84 FLLNVPWYY202 pKa = 8.66 TIVGIAVAGGLGAWFVQSAYY222 pKa = 11.06 SSGPPDD228 pKa = 3.76 LVIAGLTVIDD238 pKa = 4.44 PMVGIAIGIGVLNEE252 pKa = 4.06 LRR254 pKa = 11.84 PDD256 pKa = 3.39 VPAVLGVAMGVCAVIAIVGVVALSRR281 pKa = 11.84 YY282 pKa = 9.23 HH283 pKa = 6.81 PDD285 pKa = 3.24 VIKK288 pKa = 10.58 RR289 pKa = 11.84 RR290 pKa = 11.84 AMNKK294 pKa = 8.92 RR295 pKa = 11.84 RR296 pKa = 11.84 QKK298 pKa = 10.21 PVSTRR303 pKa = 11.84 ATPTNKK309 pKa = 9.81 PP310 pKa = 3.25
Molecular weight: 32.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.648
IPC_protein 10.628
Toseland 10.789
ProMoST 10.613
Dawson 10.877
Bjellqvist 10.643
Wikipedia 11.125
Rodwell 10.965
Grimsley 10.921
Solomon 11.067
Lehninger 11.023
Nozaki 10.789
DTASelect 10.628
Thurlkill 10.789
EMBOSS 11.213
Sillero 10.818
Patrickios 10.701
IPC_peptide 11.067
IPC2_peptide 9.882
IPC2.peptide.svr19 8.597
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3212
0
3212
1044346
37
2033
325.1
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.16 ± 0.073
0.58 ± 0.009
5.601 ± 0.037
6.042 ± 0.041
3.195 ± 0.03
9.119 ± 0.039
1.874 ± 0.02
4.22 ± 0.033
2.466 ± 0.032
10.483 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.035 ± 0.017
2.399 ± 0.025
5.518 ± 0.033
3.114 ± 0.023
6.704 ± 0.038
5.963 ± 0.031
5.702 ± 0.031
8.267 ± 0.034
1.426 ± 0.02
2.134 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here