Micromonospora rifamycinica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5460 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125Q1Y4|A0A125Q1Y4_9ACTN TetR family transcriptional regulator OS=Micromonospora rifamycinica OX=291594 GN=AWV63_05895 PE=4 SV=1
MM1 pKa = 7.45AAYY4 pKa = 9.82SRR6 pKa = 11.84LVADD10 pKa = 4.08VWADD14 pKa = 3.67PQRR17 pKa = 11.84EE18 pKa = 4.09RR19 pKa = 11.84ALEE22 pKa = 4.09SDD24 pKa = 3.45PRR26 pKa = 11.84TLLAEE31 pKa = 4.33YY32 pKa = 10.88GLGLPDD38 pKa = 3.57EE39 pKa = 4.4VRR41 pKa = 11.84VRR43 pKa = 11.84VVRR46 pKa = 11.84DD47 pKa = 3.29AADD50 pKa = 3.57TDD52 pKa = 4.05PDD54 pKa = 4.52LEE56 pKa = 4.4VQVNSWQQAADD67 pKa = 3.04TGTFVLFVPALLPVTEE83 pKa = 4.46TEE85 pKa = 4.1LAEE88 pKa = 4.8DD89 pKa = 4.02EE90 pKa = 4.69LDD92 pKa = 3.86SVVAGLDD99 pKa = 3.63SSCACCCPCCCTT111 pKa = 4.57

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A120FAB1|A0A120FAB1_9ACTN DUF218 domain-containing protein OS=Micromonospora rifamycinica OX=291594 GN=AWV63_00510 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSTRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.7GRR40 pKa = 11.84TRR42 pKa = 11.84LSAA45 pKa = 3.71

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5460

0

5460

1848823

29

8433

338.6

36.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.851 ± 0.047

0.751 ± 0.01

6.229 ± 0.027

4.815 ± 0.03

2.626 ± 0.018

9.596 ± 0.032

2.146 ± 0.017

2.92 ± 0.02

1.485 ± 0.021

10.552 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.525 ± 0.013

1.712 ± 0.02

6.51 ± 0.029

2.712 ± 0.017

8.61 ± 0.033

4.555 ± 0.022

6.457 ± 0.028

9.29 ± 0.032

1.586 ± 0.013

2.073 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski