Satellite tobacco necrosis virus C
Average proteome isoelectric point is 9.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O57031|O57031_9VIRU Coat protein (Fragment) OS=Satellite tobacco necrosis virus C OX=2025096 GN=coat PE=4 SV=1
MM1 pKa = 7.72 TKK3 pKa = 9.93 RR4 pKa = 11.84 QKK6 pKa = 9.43 RR7 pKa = 11.84 TQTTRR12 pKa = 11.84 SVRR15 pKa = 11.84 SLTTRR20 pKa = 11.84 DD21 pKa = 2.91 VRR23 pKa = 11.84 NIIRR27 pKa = 11.84 GEE29 pKa = 4.02 AEE31 pKa = 3.84 TKK33 pKa = 10.57 RR34 pKa = 11.84 FVLVNTLNLNSTAGTVLNLSNGIIQGDD61 pKa = 4.56 DD62 pKa = 2.65 ISQRR66 pKa = 11.84 AGDD69 pKa = 4.5 KK70 pKa = 10.59 IRR72 pKa = 11.84 MTKK75 pKa = 10.13 QILRR79 pKa = 11.84 VRR81 pKa = 11.84 ATAITNSQTFRR92 pKa = 11.84 FIWFKK97 pKa = 11.54 DD98 pKa = 3.14 NTNRR102 pKa = 11.84 GTTPSVTEE110 pKa = 4.06 VLNSASFMAQYY121 pKa = 11.32 NPVTLLQHH129 pKa = 6.97 RR130 pKa = 11.84 FTILKK135 pKa = 10.13 DD136 pKa = 3.58 VEE138 pKa = 4.6 LDD140 pKa = 3.51 CSLSGEE146 pKa = 4.53 SIKK149 pKa = 10.84 HH150 pKa = 5.38 LVMTHH155 pKa = 6.33 GGTSCFYY162 pKa = 11.04 NGTTAVASANGPGAIFLLVIGDD184 pKa = 4.5 SIVGTWDD191 pKa = 2.84 VGYY194 pKa = 9.44 EE195 pKa = 3.85 AHH197 pKa = 7.07 YY198 pKa = 11.12 LDD200 pKa = 5.07 LL201 pKa = 6.28
Molecular weight: 22.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.109
IPC2_protein 9.063
IPC_protein 9.136
Toseland 9.823
ProMoST 9.531
Dawson 10.028
Bjellqvist 9.736
Wikipedia 10.204
Rodwell 10.321
Grimsley 10.101
Solomon 10.087
Lehninger 10.058
Nozaki 9.882
DTASelect 9.706
Thurlkill 9.882
EMBOSS 10.233
Sillero 9.97
Patrickios 9.838
IPC_peptide 10.087
IPC2_peptide 8.478
IPC2.peptide.svr19 7.971
Protein with the highest isoelectric point:
>tr|O57031|O57031_9VIRU Coat protein (Fragment) OS=Satellite tobacco necrosis virus C OX=2025096 GN=coat PE=4 SV=1
MM1 pKa = 7.72 TKK3 pKa = 9.93 RR4 pKa = 11.84 QKK6 pKa = 9.43 RR7 pKa = 11.84 TQTTRR12 pKa = 11.84 SVRR15 pKa = 11.84 SLTTRR20 pKa = 11.84 DD21 pKa = 2.91 VRR23 pKa = 11.84 NIIRR27 pKa = 11.84 GEE29 pKa = 4.02 AEE31 pKa = 3.84 TKK33 pKa = 10.57 RR34 pKa = 11.84 FVLVNTLNLNSTAGTVLNLSNGIIQGDD61 pKa = 4.56 DD62 pKa = 2.65 ISQRR66 pKa = 11.84 AGDD69 pKa = 4.5 KK70 pKa = 10.59 IRR72 pKa = 11.84 MTKK75 pKa = 10.13 QILRR79 pKa = 11.84 VRR81 pKa = 11.84 ATAITNSQTFRR92 pKa = 11.84 FIWFKK97 pKa = 11.54 DD98 pKa = 3.14 NTNRR102 pKa = 11.84 GTTPSVTEE110 pKa = 4.06 VLNSASFMAQYY121 pKa = 11.32 NPVTLLQHH129 pKa = 6.97 RR130 pKa = 11.84 FTILKK135 pKa = 10.13 DD136 pKa = 3.58 VEE138 pKa = 4.6 LDD140 pKa = 3.51 CSLSGEE146 pKa = 4.53 SIKK149 pKa = 10.84 HH150 pKa = 5.38 LVMTHH155 pKa = 6.33 GGTSCFYY162 pKa = 11.04 NGTTAVASANGPGAIFLLVIGDD184 pKa = 4.5 SIVGTWDD191 pKa = 2.84 VGYY194 pKa = 9.44 EE195 pKa = 3.85 AHH197 pKa = 7.07 YY198 pKa = 11.12 LDD200 pKa = 5.07 LL201 pKa = 6.28
Molecular weight: 22.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.109
IPC2_protein 9.063
IPC_protein 9.136
Toseland 9.823
ProMoST 9.531
Dawson 10.028
Bjellqvist 9.736
Wikipedia 10.204
Rodwell 10.321
Grimsley 10.101
Solomon 10.087
Lehninger 10.058
Nozaki 9.882
DTASelect 9.706
Thurlkill 9.882
EMBOSS 10.233
Sillero 9.97
Patrickios 9.838
IPC_peptide 10.087
IPC2_peptide 8.478
IPC2.peptide.svr19 7.971
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
201
201
201
201.0
22.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.97 ± 0.0
0.995 ± 0.0
4.975 ± 0.0
2.985 ± 0.0
3.98 ± 0.0
7.463 ± 0.0
1.99 ± 0.0
6.965 ± 0.0
3.98 ± 0.0
8.955 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.99 ± 0.0
6.468 ± 0.0
1.493 ± 0.0
3.98 ± 0.0
7.463 ± 0.0
7.463 ± 0.0
12.438 ± 0.0
7.463 ± 0.0
0.995 ± 0.0
1.99 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here