Siphoviridae sp. ctdc_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MLD4|A0A345MLD4_9CAUD RecT protein OS=Siphoviridae sp. ctdc_1 OX=2675441 PE=4 SV=1
MM1 pKa = 7.42LFSPTTNEE9 pKa = 3.72VNEE12 pKa = 4.14MTKK15 pKa = 10.6VLIVYY20 pKa = 7.65EE21 pKa = 4.36TNPDD25 pKa = 2.91GCEE28 pKa = 3.93FYY30 pKa = 11.16LVDD33 pKa = 4.47ADD35 pKa = 3.87SHH37 pKa = 4.63QVEE40 pKa = 4.74MLEE43 pKa = 3.96QCHH46 pKa = 5.24NHH48 pKa = 6.84FINSDD53 pKa = 3.58DD54 pKa = 3.7MNEE57 pKa = 3.61GMEE60 pKa = 4.29YY61 pKa = 10.91LNIAISEE68 pKa = 4.11KK69 pKa = 10.34QYY71 pKa = 11.23LYY73 pKa = 11.05DD74 pKa = 4.05GDD76 pKa = 4.55EE77 pKa = 4.5NLPHH81 pKa = 6.97ASIWLDD87 pKa = 3.07KK88 pKa = 10.93RR89 pKa = 11.84LDD91 pKa = 3.42RR92 pKa = 11.84SEE94 pKa = 4.06PLKK97 pKa = 11.24VEE99 pKa = 4.08ADD101 pKa = 4.22LIVYY105 pKa = 8.24TGFIQQ110 pKa = 4.26

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MLD7|A0A345MLD7_9CAUD Kinase domain-containing protein OS=Siphoviridae sp. ctdc_1 OX=2675441 PE=4 SV=1
MM1 pKa = 7.3SCAEE5 pKa = 4.09VVFRR9 pKa = 11.84ALLRR13 pKa = 11.84LSQPLNLALLLCAGRR28 pKa = 11.84RR29 pKa = 11.84VSVSSHH35 pKa = 5.51CLHH38 pKa = 6.94RR39 pKa = 11.84SVSHH43 pKa = 6.75LSTVTVLLFFHH54 pKa = 6.61GVINFSVTVMRR65 pKa = 11.84FNGCVKK71 pKa = 10.58DD72 pKa = 3.8YY73 pKa = 9.73TVRR76 pKa = 11.84QAWQFTQKK84 pKa = 9.5VARR87 pKa = 11.84IFRR90 pKa = 11.84TVFKK94 pKa = 10.74FITLPPHH101 pKa = 5.84NNYY104 pKa = 9.98IGDD107 pKa = 4.1EE108 pKa = 4.03II109 pKa = 5.65

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

16476

82

932

249.6

27.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.904 ± 0.483

1.372 ± 0.16

6.179 ± 0.216

5.511 ± 0.269

3.569 ± 0.206

7.471 ± 0.411

1.93 ± 0.187

5.286 ± 0.169

4.983 ± 0.295

7.289 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.107

4.765 ± 0.247

4.455 ± 0.273

4.425 ± 0.437

5.687 ± 0.274

7.01 ± 0.277

6.209 ± 0.363

7.186 ± 0.257

1.663 ± 0.125

3.575 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski