Gammapapillomavirus 19
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AM40|A0A2D2AM40_9PAPI Protein E7 OS=Gammapapillomavirus 19 OX=1513264 GN=E7 PE=3 SV=1
MM1 pKa = 7.46 IGKK4 pKa = 8.11 EE5 pKa = 3.84 PCIGDD10 pKa = 3.65 VEE12 pKa = 4.86 LDD14 pKa = 3.47 LSEE17 pKa = 5.81 LVIPDD22 pKa = 3.77 NLLCEE27 pKa = 4.37 EE28 pKa = 4.48 SVEE31 pKa = 4.48 SLSPDD36 pKa = 3.48 CEE38 pKa = 4.31 PEE40 pKa = 3.92 EE41 pKa = 4.02 EE42 pKa = 4.42 QARR45 pKa = 11.84 STYY48 pKa = 10.59 KK49 pKa = 10.41 VDD51 pKa = 3.55 TACCFCEE58 pKa = 3.76 AAVRR62 pKa = 11.84 LCVVASVPAIHH73 pKa = 6.96 LLEE76 pKa = 4.19 EE77 pKa = 4.4 LLLGGLLLICPRR89 pKa = 11.84 CSRR92 pKa = 11.84 GPFQHH97 pKa = 6.55 GRR99 pKa = 3.41
Molecular weight: 10.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.153
IPC2_protein 4.393
IPC_protein 4.266
Toseland 4.113
ProMoST 4.355
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.05
Rodwell 4.101
Grimsley 4.037
Solomon 4.19
Lehninger 4.139
Nozaki 4.317
DTASelect 4.406
Thurlkill 4.126
EMBOSS 4.062
Sillero 4.368
Patrickios 0.693
IPC_peptide 4.202
IPC2_peptide 4.368
IPC2.peptide.svr19 4.291
Protein with the highest isoelectric point:
>tr|A0A2D2AM00|A0A2D2AM00_9PAPI Replication protein E1 OS=Gammapapillomavirus 19 OX=1513264 GN=E1 PE=3 SV=1
MM1 pKa = 7.65 MIQIKK6 pKa = 10.42 LFLIHH11 pKa = 6.57 VGNGYY16 pKa = 10.07 IIWTRR21 pKa = 11.84 MMYY24 pKa = 10.29 GKK26 pKa = 9.57 KK27 pKa = 9.9 QKK29 pKa = 10.47 GLQITMGYY37 pKa = 8.76 ILKK40 pKa = 9.75 SLMVIGHH47 pKa = 6.92 ILQYY51 pKa = 8.83 LTQMLSNMDD60 pKa = 3.73 YY61 pKa = 10.87 QDD63 pKa = 4.15 NGQSDD68 pKa = 4.34 LKK70 pKa = 10.93 ILL72 pKa = 3.82
Molecular weight: 8.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.814
IPC2_protein 9.209
IPC_protein 9.151
Toseland 9.575
ProMoST 9.399
Dawson 9.867
Bjellqvist 9.575
Wikipedia 10.072
Rodwell 10.145
Grimsley 9.984
Solomon 9.94
Lehninger 9.897
Nozaki 9.531
DTASelect 9.575
Thurlkill 9.677
EMBOSS 9.999
Sillero 9.78
Patrickios 4.673
IPC_peptide 9.926
IPC2_peptide 8.156
IPC2.peptide.svr19 8.028
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2331
72
599
333.0
37.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.834 ± 0.544
2.274 ± 0.942
6.95 ± 0.615
6.178 ± 0.511
4.848 ± 0.557
5.277 ± 0.554
1.544 ± 0.22
5.706 ± 0.661
5.277 ± 0.836
8.323 ± 0.66
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.973 ± 0.505
4.719 ± 0.594
6.049 ± 0.945
4.59 ± 0.51
5.663 ± 0.707
7.036 ± 0.806
6.092 ± 0.914
6.521 ± 0.398
1.544 ± 0.348
3.604 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here