Streptococcus phage Javan636
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6BHG1|A0A4D6BHG1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan636 OX=2548291 GN=Javan636_0007 PE=4 SV=1
MM1 pKa = 7.41 YY2 pKa = 9.21 EE3 pKa = 3.73 VLAIDD8 pKa = 4.59 FFNKK12 pKa = 9.99 AVTVKK17 pKa = 10.2 FDD19 pKa = 3.26 KK20 pKa = 10.25 EE21 pKa = 3.87 HH22 pKa = 5.7 YY23 pKa = 9.72 RR24 pKa = 11.84 IYY26 pKa = 11.03 SFDD29 pKa = 5.45 DD30 pKa = 4.16 IILMQLAHH38 pKa = 6.94 EE39 pKa = 4.58 DD40 pKa = 3.96 DD41 pKa = 3.64 YY42 pKa = 11.8 RR43 pKa = 11.84 IIYY46 pKa = 10.33 DD47 pKa = 3.6 KK48 pKa = 11.0 DD49 pKa = 2.98 IVMDD53 pKa = 4.56 ANSGEE58 pKa = 4.17 IGVVFYY64 pKa = 11.47 SEE66 pKa = 5.84 DD67 pKa = 3.32 DD68 pKa = 4.06 CAWKK72 pKa = 10.83 VKK74 pKa = 9.86 TDD76 pKa = 3.45 STEE79 pKa = 3.48 DD80 pKa = 3.16 WLYY83 pKa = 11.44 DD84 pKa = 3.37 WLGCEE89 pKa = 4.18 VLGNVYY95 pKa = 10.24 EE96 pKa = 4.42 NPDD99 pKa = 3.46 LLEE102 pKa = 4.41 SVEE105 pKa = 4.25 EE106 pKa = 4.18
Molecular weight: 12.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.91
IPC_protein 3.884
Toseland 3.668
ProMoST 4.012
Dawson 3.872
Bjellqvist 4.062
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.202
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.036
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.916
Protein with the highest isoelectric point:
>tr|A0A4D6BHK9|A0A4D6BHK9_9CAUD Antirepressor protein OS=Streptococcus phage Javan636 OX=2548291 GN=Javan636_0057 PE=4 SV=1
MM1 pKa = 7.27 NRR3 pKa = 11.84 LKK5 pKa = 10.55 EE6 pKa = 3.92 LRR8 pKa = 11.84 KK9 pKa = 9.65 EE10 pKa = 3.97 KK11 pKa = 10.81 KK12 pKa = 8.89 LTQKK16 pKa = 11.2 ALAEE20 pKa = 4.12 KK21 pKa = 10.61 LKK23 pKa = 10.58 IPYY26 pKa = 8.47 RR27 pKa = 11.84 TIQNWEE33 pKa = 4.04 NEE35 pKa = 4.24 DD36 pKa = 3.65 VQIKK40 pKa = 9.5 PDD42 pKa = 3.67 KK43 pKa = 10.74 AKK45 pKa = 10.74 QLADD49 pKa = 3.64 YY50 pKa = 10.44 FNVTIAYY57 pKa = 10.03 LLGHH61 pKa = 6.47 SDD63 pKa = 3.83 FKK65 pKa = 11.38 KK66 pKa = 10.73 SISLKK71 pKa = 10.51 DD72 pKa = 3.68 LLFHH76 pKa = 7.03 EE77 pKa = 4.71 IKK79 pKa = 10.62 GLKK82 pKa = 8.43 EE83 pKa = 3.53 YY84 pKa = 11.21 RR85 pKa = 11.84 MLRR88 pKa = 11.84 VGNEE92 pKa = 3.79 NYY94 pKa = 9.88 ISEE97 pKa = 4.34 NDD99 pKa = 3.51 VLNVISRR106 pKa = 11.84 YY107 pKa = 9.82 VNSS110 pKa = 3.73
Molecular weight: 13.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.008
IPC2_protein 8.902
IPC_protein 8.829
Toseland 9.633
ProMoST 9.311
Dawson 9.853
Bjellqvist 9.487
Wikipedia 10.014
Rodwell 10.292
Grimsley 9.926
Solomon 9.897
Lehninger 9.867
Nozaki 9.589
DTASelect 9.502
Thurlkill 9.692
EMBOSS 10.043
Sillero 9.765
Patrickios 7.629
IPC_peptide 9.897
IPC2_peptide 7.907
IPC2.peptide.svr19 8.029
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
11837
39
1527
190.9
21.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.054 ± 0.798
0.422 ± 0.078
6.353 ± 0.324
7.299 ± 0.607
4.106 ± 0.189
6.285 ± 0.594
1.276 ± 0.147
7.156 ± 0.242
8.87 ± 0.442
7.891 ± 0.278
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.441 ± 0.222
6.015 ± 0.266
2.779 ± 0.207
3.633 ± 0.193
3.751 ± 0.268
6.657 ± 0.53
6.775 ± 0.531
6.184 ± 0.238
1.217 ± 0.147
3.835 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here