Cocksfoot mottle virus (isolate Dactylis glomerata/Norway/CfMV-NO/1995) (CfMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus; Cocksfoot mottle virus

Average proteome isoelectric point is 7.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q66011|MVP_CFMVN Movement protein P1 OS=Cocksfoot mottle virus (isolate Dactylis glomerata/Norway/CfMV-NO/1995) OX=1005059 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.12GCSVVGNCKK10 pKa = 10.22SVMLMSRR17 pKa = 11.84MSWSKK22 pKa = 10.4LALLISVAMAAAMTDD37 pKa = 3.45SPPTLICMGILVSVVLNWIVCAVCEE62 pKa = 4.13EE63 pKa = 4.03ASEE66 pKa = 4.89LILGVSLEE74 pKa = 4.33TTRR77 pKa = 11.84PSPARR82 pKa = 11.84VIGEE86 pKa = 4.05PVFDD90 pKa = 3.82PRR92 pKa = 11.84YY93 pKa = 9.97GYY95 pKa = 9.65VAPAIYY101 pKa = 9.85DD102 pKa = 3.42GKK104 pKa = 10.85SFDD107 pKa = 4.03VILPISALSSASTRR121 pKa = 11.84KK122 pKa = 8.39EE123 pKa = 4.16TVEE126 pKa = 3.8MAVEE130 pKa = 4.16NSRR133 pKa = 11.84LQPLEE138 pKa = 3.92SSQTPKK144 pKa = 11.0SLVALYY150 pKa = 10.59SQDD153 pKa = 4.54LLSGWGSRR161 pKa = 11.84IKK163 pKa = 10.99GPDD166 pKa = 3.24GQEE169 pKa = 3.87YY170 pKa = 10.72LLTALHH176 pKa = 5.71VWEE179 pKa = 4.79TNISHH184 pKa = 7.22LCKK187 pKa = 10.25DD188 pKa = 3.73GKK190 pKa = 10.3KK191 pKa = 10.14VPISGCPIVASSADD205 pKa = 3.39SDD207 pKa = 4.12LDD209 pKa = 3.75FVLVSVPKK217 pKa = 10.35NAWSVLGVGVARR229 pKa = 11.84LEE231 pKa = 3.93LLKK234 pKa = 10.63RR235 pKa = 11.84RR236 pKa = 11.84TVVTVYY242 pKa = 10.92GGLDD246 pKa = 3.2SKK248 pKa = 8.32TTYY251 pKa = 10.25CATGVAEE258 pKa = 4.57LEE260 pKa = 4.04NPFRR264 pKa = 11.84IVTKK268 pKa = 9.92VTTTGGWSGSPLYY281 pKa = 10.76HH282 pKa = 7.08KK283 pKa = 10.5DD284 pKa = 3.45AIVGLHH290 pKa = 6.22LGARR294 pKa = 11.84PSAGVNRR301 pKa = 11.84ACNVAMAFRR310 pKa = 11.84VVRR313 pKa = 11.84KK314 pKa = 9.01FVTVEE319 pKa = 3.65NSEE322 pKa = 4.7LYY324 pKa = 9.86PDD326 pKa = 3.99QSSGPARR333 pKa = 11.84EE334 pKa = 4.59LDD336 pKa = 3.18AEE338 pKa = 4.74TYY340 pKa = 8.52TEE342 pKa = 4.23RR343 pKa = 11.84LEE345 pKa = 3.88QGIAFTEE352 pKa = 4.32YY353 pKa = 9.71NISGITVKK361 pKa = 10.24TSDD364 pKa = 4.4RR365 pKa = 11.84EE366 pKa = 4.31WTTAEE371 pKa = 3.86ALRR374 pKa = 11.84VARR377 pKa = 11.84YY378 pKa = 9.41KK379 pKa = 10.78PLGGGKK385 pKa = 9.65AWGDD389 pKa = 3.51SDD391 pKa = 6.47DD392 pKa = 5.18EE393 pKa = 4.48DD394 pKa = 4.09TQEE397 pKa = 4.01TAIRR401 pKa = 11.84PLNLPAGGLPTGQSALGQLIEE422 pKa = 4.08YY423 pKa = 9.95AGYY426 pKa = 8.61VWRR429 pKa = 11.84DD430 pKa = 3.16EE431 pKa = 5.22GIINSNGMPFRR442 pKa = 11.84SAGKK446 pKa = 8.13SSCRR450 pKa = 11.84FRR452 pKa = 11.84EE453 pKa = 4.21AVCRR457 pKa = 11.84AVHH460 pKa = 6.16RR461 pKa = 11.84DD462 pKa = 2.91VRR464 pKa = 11.84AAEE467 pKa = 4.23TEE469 pKa = 4.05FPEE472 pKa = 4.77LKK474 pKa = 10.23EE475 pKa = 3.84LAWPSRR481 pKa = 11.84GSKK484 pKa = 10.85AEE486 pKa = 3.79IGSLLFQAGRR496 pKa = 11.84FEE498 pKa = 4.83RR499 pKa = 11.84VEE501 pKa = 4.14APANLQLAITNLQAQYY517 pKa = 10.05PRR519 pKa = 11.84SRR521 pKa = 11.84PRR523 pKa = 11.84SCFRR527 pKa = 11.84RR528 pKa = 11.84EE529 pKa = 3.51PWCRR533 pKa = 11.84EE534 pKa = 3.76DD535 pKa = 4.88FVAEE539 pKa = 4.0IEE541 pKa = 4.64KK542 pKa = 10.04IAHH545 pKa = 5.75SGEE548 pKa = 4.31INLKK552 pKa = 10.26ASPGVPLAEE561 pKa = 4.11IGVSNQQVIDD571 pKa = 3.87VAWPLVCEE579 pKa = 4.11AVVEE583 pKa = 4.22RR584 pKa = 11.84LHH586 pKa = 7.3ALASVDD592 pKa = 3.71PRR594 pKa = 11.84QHH596 pKa = 6.25DD597 pKa = 3.55WSPEE601 pKa = 3.83EE602 pKa = 3.72LVKK605 pKa = 10.77RR606 pKa = 11.84GLCDD610 pKa = 3.38PVRR613 pKa = 11.84LFVKK617 pKa = 10.06QEE619 pKa = 3.43PHH621 pKa = 5.65SRR623 pKa = 11.84QKK625 pKa = 10.34IEE627 pKa = 3.55QGRR630 pKa = 11.84FRR632 pKa = 11.84LISSVSLVDD641 pKa = 3.53QLVEE645 pKa = 4.17RR646 pKa = 11.84MLFGPQNTTEE656 pKa = 3.72IALWHH661 pKa = 6.4SNPSKK666 pKa = 10.65PGMGLSKK673 pKa = 10.66ASQVALLWEE682 pKa = 4.51DD683 pKa = 4.16LARR686 pKa = 11.84KK687 pKa = 8.3HH688 pKa = 4.78QTHH691 pKa = 7.31PGAMADD697 pKa = 2.91ISGFDD702 pKa = 3.38WSVQDD707 pKa = 3.34WEE709 pKa = 4.3LWADD713 pKa = 3.04VSMRR717 pKa = 11.84IEE719 pKa = 4.55LGSFPALMAKK729 pKa = 10.19AAISRR734 pKa = 11.84FYY736 pKa = 11.27CLMNATFQLTNGEE749 pKa = 4.81LLTQEE754 pKa = 5.21LPGLMKK760 pKa = 10.55SGSYY764 pKa = 8.58CTSSSNSRR772 pKa = 11.84IRR774 pKa = 11.84CLMAEE779 pKa = 4.85LIGSPWCIAMGDD791 pKa = 3.8DD792 pKa = 5.11SVEE795 pKa = 3.99GWVDD799 pKa = 3.48DD800 pKa = 4.52APRR803 pKa = 11.84KK804 pKa = 9.78YY805 pKa = 10.42SALGHH810 pKa = 6.05LCKK813 pKa = 10.17EE814 pKa = 4.42YY815 pKa = 10.26EE816 pKa = 4.36ACPVLPNGDD825 pKa = 3.52LKK827 pKa = 11.05EE828 pKa = 4.18VSFCSHH834 pKa = 7.62LISKK838 pKa = 9.66GRR840 pKa = 11.84AEE842 pKa = 4.34LEE844 pKa = 4.02TWPKK848 pKa = 10.65CLFRR852 pKa = 11.84YY853 pKa = 10.24LSGPHH858 pKa = 6.57DD859 pKa = 4.0VEE861 pKa = 4.25SLEE864 pKa = 4.39MEE866 pKa = 4.61LSSSRR871 pKa = 11.84RR872 pKa = 11.84WGQIVRR878 pKa = 11.84YY879 pKa = 8.48LRR881 pKa = 11.84RR882 pKa = 11.84IGRR885 pKa = 11.84VSGNDD890 pKa = 3.46GEE892 pKa = 4.54EE893 pKa = 3.97RR894 pKa = 11.84SSNEE898 pKa = 3.59SPATTKK904 pKa = 9.49TQGSAAAWGPPQEE917 pKa = 4.16AWPVDD922 pKa = 3.66GASLSTFEE930 pKa = 4.94PSSSGWFHH938 pKa = 7.76LEE940 pKa = 3.5GWW942 pKa = 3.69

Molecular weight: 103.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q89504|P2A_CFMVN Polyprotein P2A OS=Cocksfoot mottle virus (isolate Dactylis glomerata/Norway/CfMV-NO/1995) OX=1005059 GN=ORF2A PE=1 SV=2
MM1 pKa = 7.21MVRR4 pKa = 11.84KK5 pKa = 9.39GAATKK10 pKa = 10.44APQQPKK16 pKa = 10.04PKK18 pKa = 10.12AQQQPGGRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84GRR32 pKa = 11.84SMEE35 pKa = 4.1PVSRR39 pKa = 11.84PLNPPAAVGSTLKK52 pKa = 10.65AGRR55 pKa = 11.84GRR57 pKa = 11.84TAGVSDD63 pKa = 3.66WFDD66 pKa = 2.81TGMITSYY73 pKa = 11.07LGGFQRR79 pKa = 11.84TAGTTDD85 pKa = 3.09SQVFIVSPAALDD97 pKa = 3.63RR98 pKa = 11.84VGTIAKK104 pKa = 9.89AYY106 pKa = 9.43ALWRR110 pKa = 11.84PKK112 pKa = 9.87HH113 pKa = 5.18WEE115 pKa = 3.41IVYY118 pKa = 10.19LPRR121 pKa = 11.84CSTQTDD127 pKa = 3.86GSIEE131 pKa = 3.98MGFLLDD137 pKa = 4.08YY138 pKa = 10.97ADD140 pKa = 4.32SVPTNTRR147 pKa = 11.84TMASSTSFTTSNVWGGGDD165 pKa = 3.72GSSLLHH171 pKa = 6.06TSVKK175 pKa = 10.89SMGNAVTSALPCDD188 pKa = 3.66EE189 pKa = 5.84FSNKK193 pKa = 7.29WFKK196 pKa = 11.1LSWSTPEE203 pKa = 3.78EE204 pKa = 4.32SEE206 pKa = 4.28NAHH209 pKa = 5.97LTDD212 pKa = 3.53TYY214 pKa = 10.7VPARR218 pKa = 11.84FVVRR222 pKa = 11.84SDD224 pKa = 3.89FPVVTADD231 pKa = 3.94QPGHH235 pKa = 5.59LWLRR239 pKa = 11.84SRR241 pKa = 11.84ILLKK245 pKa = 10.9GSVSPSTNLL254 pKa = 3.32

Molecular weight: 27.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1872

108

942

468.0

51.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.013 ± 0.936

2.404 ± 0.501

4.274 ± 0.133

6.25 ± 0.963

2.671 ± 0.603

7.532 ± 0.266

1.389 ± 0.265

3.953 ± 0.358

4.594 ± 0.498

8.814 ± 0.821

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.176

2.564 ± 0.091

6.143 ± 0.303

2.938 ± 0.176

6.517 ± 0.285

11.271 ± 0.841

6.09 ± 1.112

7.959 ± 0.372

2.35 ± 0.321

2.244 ± 0.063
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski