Modestobacter marinus
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5435 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I4ETU0|I4ETU0_9ACTN FAD-binding 9 siderophore-interacting protein OS=Modestobacter marinus OX=477641 GN=viuB PE=4 SV=1
MM1 pKa = 7.69 SSATRR6 pKa = 11.84 PALAALGLAAGLLLAGCGGDD26 pKa = 3.82 ADD28 pKa = 4.14 ATAEE32 pKa = 4.14 GHH34 pKa = 5.89 DD35 pKa = 4.41 HH36 pKa = 6.77 GGASAPATVEE46 pKa = 4.06 GGDD49 pKa = 3.52 DD50 pKa = 3.03 SGYY53 pKa = 11.3 AGLHH57 pKa = 6.18 LADD60 pKa = 5.78 PYY62 pKa = 11.02 QKK64 pKa = 10.74 PEE66 pKa = 3.79 FTLTDD71 pKa = 3.39 SAGAPFDD78 pKa = 3.62 FADD81 pKa = 4.08 RR82 pKa = 11.84 TGTGPTLLFFGYY94 pKa = 7.92 TNCPDD99 pKa = 3.37 VCPTTMADD107 pKa = 2.96 VALALRR113 pKa = 11.84 KK114 pKa = 9.67 VDD116 pKa = 3.61 PAVAAEE122 pKa = 4.05 TSVVFVTTDD131 pKa = 3.5 PARR134 pKa = 11.84 DD135 pKa = 3.65 TPAVLGAYY143 pKa = 10.06 LDD145 pKa = 3.84 QFDD148 pKa = 5.58 ADD150 pKa = 4.38 LATQFTGLTGDD161 pKa = 3.54 QAQVDD166 pKa = 4.31 QAQLAAGVPLAEE178 pKa = 6.14 DD179 pKa = 3.47 MGQTHH184 pKa = 7.02 SSLLLLYY191 pKa = 9.06 GTDD194 pKa = 3.64 GEE196 pKa = 4.56 ADD198 pKa = 3.57 VAFDD202 pKa = 4.77 AGNTGADD209 pKa = 3.3 IAHH212 pKa = 7.22 DD213 pKa = 4.24 LALVAAGG220 pKa = 3.86
Molecular weight: 22.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.465
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.694
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.139
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.834
Patrickios 1.074
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|I4EQ22|I4EQ22_9ACTN DUF3566 domain-containing protein OS=Modestobacter marinus OX=477641 GN=MODMU_0013 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 8.58 THH17 pKa = 5.46 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAGRR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.9 GRR40 pKa = 11.84 AKK42 pKa = 10.73 LSAA45 pKa = 3.92
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5435
0
5435
1684791
22
1844
310.0
32.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.564 ± 0.05
0.694 ± 0.009
6.235 ± 0.03
5.228 ± 0.035
2.52 ± 0.018
9.639 ± 0.033
2.045 ± 0.016
2.635 ± 0.025
1.285 ± 0.02
10.804 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.566 ± 0.013
1.388 ± 0.016
6.29 ± 0.034
2.855 ± 0.019
8.026 ± 0.036
4.975 ± 0.025
6.157 ± 0.027
9.873 ± 0.034
1.516 ± 0.014
1.705 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here