Escherichia phage vB_EcoS_AKS96
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A067YXX0|A0A067YXX0_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_AKS96 OX=1416031 GN=AKS96_40 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.38 AIIINNEE9 pKa = 2.81 IRR11 pKa = 11.84 YY12 pKa = 9.99 DD13 pKa = 4.01 EE14 pKa = 5.36 DD15 pKa = 4.13 SMSLAEE21 pKa = 4.91 LGYY24 pKa = 10.56 QIGEE28 pKa = 4.03 EE29 pKa = 4.26 VEE31 pKa = 4.27 VYY33 pKa = 10.14 EE34 pKa = 4.9 IEE36 pKa = 4.43 GFYY39 pKa = 10.58 MMSLKK44 pKa = 10.84 DD45 pKa = 3.5 HH46 pKa = 6.56 VYY48 pKa = 10.84 VDD50 pKa = 3.43 GTLAIFQGEE59 pKa = 4.15 QFVVSNEE66 pKa = 4.09 EE67 pKa = 3.95 IEE69 pKa = 4.67 VVAA72 pKa = 5.4
Molecular weight: 8.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.804
IPC2_protein 3.935
IPC_protein 3.783
Toseland 3.63
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.63
Grimsley 3.554
Solomon 3.706
Lehninger 3.668
Nozaki 3.872
DTASelect 3.935
Thurlkill 3.668
EMBOSS 3.63
Sillero 3.897
Patrickios 0.693
IPC_peptide 3.719
IPC2_peptide 3.884
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A067YYH6|A0A067YYH6_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_AKS96 OX=1416031 GN=AKS96_39 PE=4 SV=1
MM1 pKa = 8.11 DD2 pKa = 6.16 SIDD5 pKa = 4.34 KK6 pKa = 9.33 RR7 pKa = 11.84 TLNGNNGTIRR17 pKa = 11.84 TEE19 pKa = 3.92 DD20 pKa = 3.06 KK21 pKa = 10.74 KK22 pKa = 10.7 PRR24 pKa = 11.84 KK25 pKa = 9.27 RR26 pKa = 11.84 PSGYY30 pKa = 10.17 YY31 pKa = 8.95 VLKK34 pKa = 10.9 DD35 pKa = 3.51 EE36 pKa = 4.52 VRR38 pKa = 11.84 AGLRR42 pKa = 11.84 ARR44 pKa = 11.84 LEE46 pKa = 4.11 IVLDD50 pKa = 4.33 FYY52 pKa = 11.64 GSKK55 pKa = 11.11 ANLAKK60 pKa = 10.04 QLKK63 pKa = 6.67 VTRR66 pKa = 11.84 QAVEE70 pKa = 4.15 EE71 pKa = 4.13 WCKK74 pKa = 10.82 RR75 pKa = 11.84 GMISARR81 pKa = 11.84 GAQLAHH87 pKa = 5.93 NRR89 pKa = 11.84 YY90 pKa = 9.16 KK91 pKa = 10.35 RR92 pKa = 11.84 TGEE95 pKa = 3.84 GFRR98 pKa = 11.84 ATFCRR103 pKa = 11.84 PDD105 pKa = 3.38 LQFDD109 pKa = 4.36 GNGKK113 pKa = 8.92 PLTLRR118 pKa = 11.84 CKK120 pKa = 9.57 KK121 pKa = 10.07 RR122 pKa = 11.84 QMLRR126 pKa = 11.84 VVTEE130 pKa = 4.28 AEE132 pKa = 4.41 LATKK136 pKa = 8.4 PQRR139 pKa = 11.84 KK140 pKa = 7.72 SWRR143 pKa = 11.84 KK144 pKa = 7.89 VKK146 pKa = 10.54 KK147 pKa = 9.7 EE148 pKa = 3.59 RR149 pKa = 11.84 EE150 pKa = 3.98 EE151 pKa = 3.69 ARR153 pKa = 11.84 KK154 pKa = 10.06 AKK156 pKa = 9.88 EE157 pKa = 3.53
Molecular weight: 18.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.604
IPC_protein 9.897
Toseland 10.745
ProMoST 10.277
Dawson 10.804
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.228
Grimsley 10.833
Solomon 10.877
Lehninger 10.862
Nozaki 10.716
DTASelect 10.423
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.73
Patrickios 10.95
IPC_peptide 10.891
IPC2_peptide 9.136
IPC2.peptide.svr19 8.724
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
13991
38
1128
189.1
21.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.248 ± 0.471
1.287 ± 0.213
6.233 ± 0.235
6.383 ± 0.387
3.953 ± 0.185
7.734 ± 0.365
1.451 ± 0.226
6.483 ± 0.238
6.39 ± 0.467
7.005 ± 0.251
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.652 ± 0.335
5.218 ± 0.272
3.002 ± 0.222
3.874 ± 0.296
4.846 ± 0.189
7.269 ± 0.395
5.539 ± 0.285
7.262 ± 0.265
1.229 ± 0.11
3.945 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here