Burkholderia phage BEK

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Tigrvirus; Burkholderia virus phi52237

Average proteome isoelectric point is 7.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P1QFK6|A0A4P1QFK6_9CAUD Uncharacterized protein OS=Burkholderia phage BEK OX=1514988 GN=DP46_6040 PE=4 SV=1
MM1 pKa = 7.35LVFVNEE7 pKa = 3.98GRR9 pKa = 11.84IEE11 pKa = 4.06ATGTRR16 pKa = 11.84TASFDD21 pKa = 3.57YY22 pKa = 10.55EE23 pKa = 4.45YY24 pKa = 10.09EE25 pKa = 4.1CEE27 pKa = 3.94IIIRR31 pKa = 11.84DD32 pKa = 4.52FIGNPDD38 pKa = 3.7DD39 pKa = 4.59VMIAVVEE46 pKa = 4.21WARR49 pKa = 11.84ANQPDD54 pKa = 4.44LVTNRR59 pKa = 11.84DD60 pKa = 3.08EE61 pKa = 4.6RR62 pKa = 11.84RR63 pKa = 11.84NGMTFVADD71 pKa = 3.33ILSNNAVDD79 pKa = 5.23LGLKK83 pKa = 9.89VKK85 pKa = 10.66LSEE88 pKa = 4.35SVVVGTDD95 pKa = 2.99EE96 pKa = 4.33AGNRR100 pKa = 11.84TVEE103 pKa = 4.78HH104 pKa = 6.93IDD106 pKa = 3.61DD107 pKa = 5.43AADD110 pKa = 3.14EE111 pKa = 4.32WLSS114 pKa = 3.44

Molecular weight: 12.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P1QFK3|A0A4P1QFK3_9CAUD Uncharacterized protein OS=Burkholderia phage BEK OX=1514988 GN=DP46_6006 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84SLISPQSRR12 pKa = 11.84SPSLKK17 pKa = 10.31AKK19 pKa = 9.92RR20 pKa = 11.84SPNFRR25 pKa = 11.84PITPKK30 pKa = 10.75GG31 pKa = 3.36

Molecular weight: 3.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

9369

31

930

176.8

19.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.659 ± 0.516

1.185 ± 0.165

6.127 ± 0.315

5.337 ± 0.233

3.533 ± 0.228

7.247 ± 0.261

2.487 ± 0.236

4.184 ± 0.208

4.013 ± 0.301

8.987 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.136

2.925 ± 0.266

4.878 ± 0.298

3.287 ± 0.218

8.806 ± 0.416

5.39 ± 0.246

5.828 ± 0.307

6.724 ± 0.281

1.633 ± 0.154

2.572 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski