Pedobacter glucosidilyticus
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3311 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B8XS80|A0A0B8XS80_9SPHI Putative enoyl-CoA hydratase OS=Pedobacter glucosidilyticus OX=1122941 GN=fadB PE=3 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.21 LASHH10 pKa = 7.01 LEE12 pKa = 4.08 DD13 pKa = 4.93 APWPATKK20 pKa = 10.54 DD21 pKa = 3.44 EE22 pKa = 5.26 LIDD25 pKa = 3.48 YY26 pKa = 8.56 AIRR29 pKa = 11.84 SGAPVEE35 pKa = 4.53 VIEE38 pKa = 4.56 NLQALEE44 pKa = 4.69 DD45 pKa = 4.28 DD46 pKa = 4.31 GEE48 pKa = 4.31 PYY50 pKa = 11.05 EE51 pKa = 4.65 NIEE54 pKa = 4.81 EE55 pKa = 4.08 IWPDD59 pKa = 3.85 YY60 pKa = 7.47 PTKK63 pKa = 10.94 DD64 pKa = 3.05 DD65 pKa = 4.15 FFFNEE70 pKa = 4.51 DD71 pKa = 3.17 EE72 pKa = 4.46 YY73 pKa = 11.99
Molecular weight: 8.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.592
IPC_protein 3.528
Toseland 3.338
ProMoST 3.694
Dawson 3.516
Bjellqvist 3.681
Wikipedia 3.439
Rodwell 3.363
Grimsley 3.261
Solomon 3.478
Lehninger 3.439
Nozaki 3.643
DTASelect 3.795
Thurlkill 3.401
EMBOSS 3.452
Sillero 3.656
Patrickios 0.693
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.629
Protein with the highest isoelectric point:
>tr|A0A0B8XNS0|A0A0B8XNS0_9SPHI UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase OS=Pedobacter glucosidilyticus OX=1122941 GN=PBAC_25870 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.39 KK6 pKa = 9.62 RR7 pKa = 11.84 KK8 pKa = 6.72 RR9 pKa = 11.84 HH10 pKa = 5.17 KK11 pKa = 10.05 MATHH15 pKa = 6.02 KK16 pKa = 10.34 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.023
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.705
Grimsley 12.764
Solomon 13.217
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.427
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.989
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3311
0
3311
1165797
29
5423
352.1
39.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.239 ± 0.041
0.756 ± 0.014
5.276 ± 0.029
5.968 ± 0.043
5.211 ± 0.032
6.497 ± 0.051
1.83 ± 0.026
7.812 ± 0.034
7.698 ± 0.042
9.678 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.064 ± 0.022
5.999 ± 0.046
3.576 ± 0.025
3.845 ± 0.029
3.493 ± 0.028
6.268 ± 0.039
5.562 ± 0.046
6.027 ± 0.032
1.145 ± 0.016
4.053 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here