Amino acid dipepetide frequency for Lactobacillus bifermentans DSM 20003

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.28AlaAla: 11.28 ± 0.247	0.595AlaCys: 0.595 ± 0.031	5.703AlaAsp: 5.703 ± 0.096	4.514AlaGlu: 4.514 ± 0.076	3.649AlaPhe: 3.649 ± 0.063	6.925AlaGly: 6.925 ± 0.102	1.952AlaHis: 1.952 ± 0.047	6.014AlaIle: 6.014 ± 0.095	5.576AlaLys: 5.576 ± 0.108	10.336AlaLeu: 10.336 ± 0.128	2.432AlaMet: 2.432 ± 0.057	3.735AlaAsn: 3.735 ± 0.086	3.329AlaPro: 3.329 ± 0.066	6.191AlaGln: 6.191 ± 0.123	3.105AlaArg: 3.105 ± 0.057	4.898AlaSer: 4.898 ± 0.093	7.373AlaThr: 7.373 ± 0.153	6.573AlaVal: 6.573 ± 0.096	0.937AlaTrp: 0.937 ± 0.032	3.016AlaTyr: 3.016 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.501CysAla: 0.501 ± 0.024	0.088CysCys: 0.088 ± 0.012	0.36CysAsp: 0.36 ± 0.02	0.298CysGlu: 0.298 ± 0.021	0.291CysPhe: 0.291 ± 0.02	0.624CysGly: 0.624 ± 0.031	0.222CysHis: 0.222 ± 0.018	0.281CysIle: 0.281 ± 0.018	0.169CysLys: 0.169 ± 0.013	0.747CysLeu: 0.747 ± 0.032	0.12CysMet: 0.12 ± 0.014	0.19CysAsn: 0.19 ± 0.015	0.28CysPro: 0.28 ± 0.02	0.418CysGln: 0.418 ± 0.024	0.294CysArg: 0.294 ± 0.022	0.321CysSer: 0.321 ± 0.02	0.32CysThr: 0.32 ± 0.022	0.393CysVal: 0.393 ± 0.022	0.103CysTrp: 0.103 ± 0.012	0.269CysTyr: 0.269 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.522AspAla: 4.522 ± 0.088	0.325AspCys: 0.325 ± 0.02	3.08AspAsp: 3.08 ± 0.08	3.095AspGlu: 3.095 ± 0.07	2.706AspPhe: 2.706 ± 0.057	3.171AspGly: 3.171 ± 0.078	1.44AspHis: 1.44 ± 0.046	2.837AspIle: 2.837 ± 0.057	2.494AspLys: 2.494 ± 0.067	5.807AspLeu: 5.807 ± 0.098	1.305AspMet: 1.305 ± 0.037	1.818AspAsn: 1.818 ± 0.052	2.28AspPro: 2.28 ± 0.056	3.761AspGln: 3.761 ± 0.079	2.102AspArg: 2.102 ± 0.05	2.285AspSer: 2.285 ± 0.055	3.046AspThr: 3.046 ± 0.075	3.818AspVal: 3.818 ± 0.085	0.798AspTrp: 0.798 ± 0.034	2.438AspTyr: 2.438 ± 0.063	0.002AspXaa: 0.002 ± 0.002
Glu	4.563GluAla: 4.563 ± 0.087	0.193GluCys: 0.193 ± 0.016	2.052GluAsp: 2.052 ± 0.056	1.977GluGlu: 1.977 ± 0.056	1.73GluPhe: 1.73 ± 0.053	2.204GluGly: 2.204 ± 0.049	1.09GluHis: 1.09 ± 0.041	3.201GluIle: 3.201 ± 0.071	3.175GluLys: 3.175 ± 0.062	5.038GluLeu: 5.038 ± 0.08	1.435GluMet: 1.435 ± 0.044	2.216GluAsn: 2.216 ± 0.055	1.66GluPro: 1.66 ± 0.047	2.892GluGln: 2.892 ± 0.069	2.292GluArg: 2.292 ± 0.059	2.058GluSer: 2.058 ± 0.06	3.383GluThr: 3.383 ± 0.067	3.167GluVal: 3.167 ± 0.07	0.417GluTrp: 0.417 ± 0.024	1.262GluTyr: 1.262 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.395PheAla: 3.395 ± 0.061	0.347PheCys: 0.347 ± 0.021	2.511PheAsp: 2.511 ± 0.054	2.171PheGlu: 2.171 ± 0.055	1.765PhePhe: 1.765 ± 0.052	3.377PheGly: 3.377 ± 0.07	0.877PheHis: 0.877 ± 0.031	2.521PheIle: 2.521 ± 0.062	2.41PheLys: 2.41 ± 0.044	3.719PheLeu: 3.719 ± 0.082	1.113PheMet: 1.113 ± 0.036	1.857PheAsn: 1.857 ± 0.046	1.449PhePro: 1.449 ± 0.041	1.909PheGln: 1.909 ± 0.05	1.297PheArg: 1.297 ± 0.041	2.519PheSer: 2.519 ± 0.059	2.568PheThr: 2.568 ± 0.055	2.962PheVal: 2.962 ± 0.071	0.552PheTrp: 0.552 ± 0.028	1.492PheTyr: 1.492 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	5.739GlyAla: 5.739 ± 0.09	0.541GlyCys: 0.541 ± 0.026	3.32GlyAsp: 3.32 ± 0.064	2.91GlyGlu: 2.91 ± 0.071	3.051GlyPhe: 3.051 ± 0.065	4.456GlyGly: 4.456 ± 0.082	1.63GlyHis: 1.63 ± 0.04	4.96GlyIle: 4.96 ± 0.09	3.666GlyLys: 3.666 ± 0.071	7.34GlyLeu: 7.34 ± 0.107	1.936GlyMet: 1.936 ± 0.054	2.441GlyAsn: 2.441 ± 0.054	1.758GlyPro: 1.758 ± 0.049	3.776GlyGln: 3.776 ± 0.066	2.586GlyArg: 2.586 ± 0.062	3.68GlySer: 3.68 ± 0.085	4.761GlyThr: 4.761 ± 0.127	5.014GlyVal: 5.014 ± 0.093	0.824GlyTrp: 0.824 ± 0.033	2.795GlyTyr: 2.795 ± 0.075	0.001GlyXaa: 0.001 ± 0.001
His	1.708HisAla: 1.708 ± 0.042	0.192HisCys: 0.192 ± 0.016	1.287HisAsp: 1.287 ± 0.041	1.076HisGlu: 1.076 ± 0.036	1.289HisPhe: 1.289 ± 0.038	1.554HisGly: 1.554 ± 0.044	0.817HisHis: 0.817 ± 0.032	1.214HisIle: 1.214 ± 0.037	0.866HisLys: 0.866 ± 0.03	2.545HisLeu: 2.545 ± 0.055	0.51HisMet: 0.51 ± 0.025	0.882HisAsn: 0.882 ± 0.026	1.078HisPro: 1.078 ± 0.039	1.636HisGln: 1.636 ± 0.045	0.929HisArg: 0.929 ± 0.036	0.978HisSer: 0.978 ± 0.036	1.161HisThr: 1.161 ± 0.035	1.555HisVal: 1.555 ± 0.046	0.374HisTrp: 0.374 ± 0.024	1.26HisTyr: 1.26 ± 0.045	0.0HisXaa: 0.0 ± 0.0
Ile	6.068IleAla: 6.068 ± 0.096	0.507IleCys: 0.507 ± 0.024	3.705IleAsp: 3.705 ± 0.076	2.999IleGlu: 2.999 ± 0.058	2.571IlePhe: 2.571 ± 0.062	5.053IleGly: 5.053 ± 0.094	1.204IleHis: 1.204 ± 0.039	3.702IleIle: 3.702 ± 0.072	3.261IleLys: 3.261 ± 0.063	5.943IleLeu: 5.943 ± 0.107	1.522IleMet: 1.522 ± 0.046	2.658IleAsn: 2.658 ± 0.065	2.474IlePro: 2.474 ± 0.059	3.027IleGln: 3.027 ± 0.069	2.405IleArg: 2.405 ± 0.051	3.566IleSer: 3.566 ± 0.067	4.288IleThr: 4.288 ± 0.102	4.548IleVal: 4.548 ± 0.085	0.596IleTrp: 0.596 ± 0.027	1.87IleTyr: 1.87 ± 0.048	0.001IleXaa: 0.001 ± 0.001
Lys	5.246LysAla: 5.246 ± 0.096	0.189LysCys: 0.189 ± 0.016	2.49LysAsp: 2.49 ± 0.068	2.414LysGlu: 2.414 ± 0.058	1.572LysPhe: 1.572 ± 0.043	3.052LysGly: 3.052 ± 0.064	1.032LysHis: 1.032 ± 0.037	3.483LysIle: 3.483 ± 0.073	3.41LysLys: 3.41 ± 0.082	4.899LysLeu: 4.899 ± 0.085	1.719LysMet: 1.719 ± 0.043	2.276LysAsn: 2.276 ± 0.055	2.023LysPro: 2.023 ± 0.052	3.614LysGln: 3.614 ± 0.075	2.879LysArg: 2.879 ± 0.064	2.649LysSer: 2.649 ± 0.089	3.942LysThr: 3.942 ± 0.086	3.772LysVal: 3.772 ± 0.07	0.533LysTrp: 0.533 ± 0.023	1.634LysTyr: 1.634 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	11.485LeuAla: 11.485 ± 0.139	0.694LeuCys: 0.694 ± 0.033	5.139LeuAsp: 5.139 ± 0.091	4.117LeuGlu: 4.117 ± 0.078	4.115LeuPhe: 4.115 ± 0.09	7.063LeuGly: 7.063 ± 0.117	1.992LeuHis: 1.992 ± 0.049	6.731LeuIle: 6.731 ± 0.126	6.275LeuLys: 6.275 ± 0.109	10.747LeuLeu: 10.747 ± 0.181	2.589LeuMet: 2.589 ± 0.06	4.462LeuAsn: 4.462 ± 0.083	5.073LeuPro: 5.073 ± 0.085	5.386LeuGln: 5.386 ± 0.123	4.095LeuArg: 4.095 ± 0.077	5.85LeuSer: 5.85 ± 0.086	8.327LeuThr: 8.327 ± 0.116	7.122LeuVal: 7.122 ± 0.115	0.971LeuTrp: 0.971 ± 0.044	2.854LeuTyr: 2.854 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.676MetAla: 2.676 ± 0.054	0.168MetCys: 0.168 ± 0.014	1.091MetAsp: 1.091 ± 0.037	0.934MetGlu: 0.934 ± 0.034	0.78MetPhe: 0.78 ± 0.031	1.601MetGly: 1.601 ± 0.045	0.452MetHis: 0.452 ± 0.022	1.717MetIle: 1.717 ± 0.047	1.495MetLys: 1.495 ± 0.042	2.428MetLeu: 2.428 ± 0.054	0.744MetMet: 0.744 ± 0.03	1.102MetAsn: 1.102 ± 0.037	1.102MetPro: 1.102 ± 0.036	1.276MetGln: 1.276 ± 0.039	1.057MetArg: 1.057 ± 0.035	1.414MetSer: 1.414 ± 0.041	2.166MetThr: 2.166 ± 0.043	1.719MetVal: 1.719 ± 0.047	0.193MetTrp: 0.193 ± 0.017	0.592MetTyr: 0.592 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.479AsnAla: 3.479 ± 0.076	0.278AsnCys: 0.278 ± 0.019	2.198AsnAsp: 2.198 ± 0.06	1.968AsnGlu: 1.968 ± 0.053	1.599AsnPhe: 1.599 ± 0.046	3.259AsnGly: 3.259 ± 0.07	1.021AsnHis: 1.021 ± 0.038	2.341AsnIle: 2.341 ± 0.048	1.783AsnLys: 1.783 ± 0.049	3.9AsnLeu: 3.9 ± 0.074	1.035AsnMet: 1.035 ± 0.037	1.564AsnAsn: 1.564 ± 0.047	1.825AsnPro: 1.825 ± 0.054	2.695AsnGln: 2.695 ± 0.063	1.882AsnArg: 1.882 ± 0.047	1.877AsnSer: 1.877 ± 0.052	2.38AsnThr: 2.38 ± 0.062	2.797AsnVal: 2.797 ± 0.058	0.597AsnTrp: 0.597 ± 0.027	1.518AsnTyr: 1.518 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	4.337ProAla: 4.337 ± 0.091	0.18ProCys: 0.18 ± 0.014	2.63ProAsp: 2.63 ± 0.062	2.736ProGlu: 2.736 ± 0.058	1.716ProPhe: 1.716 ± 0.043	2.414ProGly: 2.414 ± 0.061	0.843ProHis: 0.843 ± 0.032	2.422ProIle: 2.422 ± 0.055	2.069ProLys: 2.069 ± 0.056	3.976ProLeu: 3.976 ± 0.083	0.816ProMet: 0.816 ± 0.037	1.663ProAsn: 1.663 ± 0.05	0.727ProPro: 0.727 ± 0.03	2.28ProGln: 2.28 ± 0.055	1.35ProArg: 1.35 ± 0.042	1.81ProSer: 1.81 ± 0.048	3.033ProThr: 3.033 ± 0.082	3.157ProVal: 3.157 ± 0.077	0.358ProTrp: 0.358 ± 0.019	1.366ProTyr: 1.366 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	7.098GlnAla: 7.098 ± 0.124	0.228GlnCys: 0.228 ± 0.016	2.257GlnAsp: 2.257 ± 0.054	2.174GlnGlu: 2.174 ± 0.055	2.213GlnPhe: 2.213 ± 0.053	2.848GlnGly: 2.848 ± 0.061	1.784GlnHis: 1.784 ± 0.052	3.571GlnIle: 3.571 ± 0.077	2.919GlnLys: 2.919 ± 0.062	7.239GlnLeu: 7.239 ± 0.166	1.271GlnMet: 1.271 ± 0.04	2.152GlnAsn: 2.152 ± 0.053	2.769GlnPro: 2.769 ± 0.064	5.702GlnGln: 5.702 ± 0.188	3.426GlnArg: 3.426 ± 0.082	2.486GlnSer: 2.486 ± 0.06	4.37GlnThr: 4.37 ± 0.096	4.516GlnVal: 4.516 ± 0.096	0.62GlnTrp: 0.62 ± 0.029	1.81GlnTyr: 1.81 ± 0.052	0.0GlnXaa: 0.0 ± 0.0
Arg	2.952ArgAla: 2.952 ± 0.075	0.245ArgCys: 0.245 ± 0.021	2.165ArgAsp: 2.165 ± 0.056	2.048ArgGlu: 2.048 ± 0.054	1.904ArgPhe: 1.904 ± 0.051	2.231ArgGly: 2.231 ± 0.054	1.251ArgHis: 1.251 ± 0.039	2.471ArgIle: 2.471 ± 0.059	2.114ArgLys: 2.114 ± 0.053	4.837ArgLeu: 4.837 ± 0.103	1.034ArgMet: 1.034 ± 0.034	1.608ArgAsn: 1.608 ± 0.047	1.705ArgPro: 1.705 ± 0.046	3.375ArgGln: 3.375 ± 0.077	2.311ArgArg: 2.311 ± 0.049	1.838ArgSer: 1.838 ± 0.045	2.241ArgThr: 2.241 ± 0.057	2.604ArgVal: 2.604 ± 0.059	0.527ArgTrp: 0.527 ± 0.026	1.792ArgTyr: 1.792 ± 0.048	0.002ArgXaa: 0.002 ± 0.002
Ser	4.653SerAla: 4.653 ± 0.111	0.321SerCys: 0.321 ± 0.02	3.003SerAsp: 3.003 ± 0.075	2.667SerGlu: 2.667 ± 0.054	2.26SerPhe: 2.26 ± 0.05	4.242SerGly: 4.242 ± 0.094	1.142SerHis: 1.142 ± 0.04	2.963SerIle: 2.963 ± 0.066	2.541SerLys: 2.541 ± 0.07	5.348SerLeu: 5.348 ± 0.086	1.203SerMet: 1.203 ± 0.04	2.085SerAsn: 2.085 ± 0.056	1.744SerPro: 1.744 ± 0.046	2.701SerGln: 2.701 ± 0.065	2.047SerArg: 2.047 ± 0.053	3.0SerSer: 3.0 ± 0.11	3.261SerThr: 3.261 ± 0.08	3.613SerVal: 3.613 ± 0.07	0.587SerTrp: 0.587 ± 0.026	1.756SerTyr: 1.756 ± 0.047	0.001SerXaa: 0.001 ± 0.001
Thr	7.444ThrAla: 7.444 ± 0.147	0.343ThrCys: 0.343 ± 0.018	3.925ThrAsp: 3.925 ± 0.092	3.167ThrGlu: 3.167 ± 0.062	2.717ThrPhe: 2.717 ± 0.064	5.13ThrGly: 5.13 ± 0.102	1.382ThrHis: 1.382 ± 0.039	4.465ThrIle: 4.465 ± 0.108	3.587ThrLys: 3.587 ± 0.069	7.313ThrLeu: 7.313 ± 0.105	1.385ThrMet: 1.385 ± 0.037	2.679ThrAsn: 2.679 ± 0.071	3.617ThrPro: 3.617 ± 0.108	3.621ThrGln: 3.621 ± 0.085	2.206ThrArg: 2.206 ± 0.056	3.641ThrSer: 3.641 ± 0.105	5.864ThrThr: 5.864 ± 0.182	5.59ThrVal: 5.59 ± 0.17	0.709ThrTrp: 0.709 ± 0.027	2.329ThrTyr: 2.329 ± 0.061	0.0ThrXaa: 0.0 ± 0.0
Val	7.559ValAla: 7.559 ± 0.116	0.5ValCys: 0.5 ± 0.028	3.723ValAsp: 3.723 ± 0.074	2.859ValGlu: 2.859 ± 0.055	2.636ValPhe: 2.636 ± 0.067	4.926ValGly: 4.926 ± 0.082	1.383ValHis: 1.383 ± 0.043	4.839ValIle: 4.839 ± 0.084	3.603ValLys: 3.603 ± 0.066	7.265ValLeu: 7.265 ± 0.1	1.686ValMet: 1.686 ± 0.042	2.883ValAsn: 2.883 ± 0.085	3.122ValPro: 3.122 ± 0.087	3.353ValGln: 3.353 ± 0.067	2.484ValArg: 2.484 ± 0.054	4.06ValSer: 4.06 ± 0.074	6.032ValThr: 6.032 ± 0.152	5.376ValVal: 5.376 ± 0.093	0.631ValTrp: 0.631 ± 0.027	2.178ValTyr: 2.178 ± 0.064	0.001ValXaa: 0.001 ± 0.001
Trp	0.767TrpAla: 0.767 ± 0.028	0.112TrpCys: 0.112 ± 0.013	0.444TrpAsp: 0.444 ± 0.024	0.404TrpGlu: 0.404 ± 0.021	0.514TrpPhe: 0.514 ± 0.026	0.667TrpGly: 0.667 ± 0.029	0.382TrpHis: 0.382 ± 0.021	0.57TrpIle: 0.57 ± 0.029	0.291TrpLys: 0.291 ± 0.02	1.746TrpLeu: 1.746 ± 0.06	0.199TrpMet: 0.199 ± 0.016	0.413TrpAsn: 0.413 ± 0.022	0.438TrpPro: 0.438 ± 0.021	1.108TrpGln: 1.108 ± 0.037	0.642TrpArg: 0.642 ± 0.025	0.539TrpSer: 0.539 ± 0.026	0.565TrpThr: 0.565 ± 0.028	0.637TrpVal: 0.637 ± 0.028	0.19TrpTrp: 0.19 ± 0.016	0.352TrpTyr: 0.352 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.66TyrAla: 2.66 ± 0.057	0.244TyrCys: 0.244 ± 0.019	2.05TyrAsp: 2.05 ± 0.058	1.477TyrGlu: 1.477 ± 0.05	1.733TyrPhe: 1.733 ± 0.049	2.39TyrGly: 2.39 ± 0.058	1.016TyrHis: 1.016 ± 0.037	1.663TyrIle: 1.663 ± 0.041	1.125TyrLys: 1.125 ± 0.044	3.915TyrLeu: 3.915 ± 0.083	0.647TyrMet: 0.647 ± 0.03	1.309TyrAsn: 1.309 ± 0.046	1.429TyrPro: 1.429 ± 0.043	2.731TyrGln: 2.731 ± 0.06	1.861TyrArg: 1.861 ± 0.049	1.669TyrSer: 1.669 ± 0.05	2.034TyrThr: 2.034 ± 0.088	2.167TyrVal: 2.167 ± 0.059	0.461TyrTrp: 0.461 ± 0.024	1.3TyrTyr: 1.3 ± 0.046	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.002	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.135XaaXaa: 0.135 ± 0.067

Statistics based on 2964 proteins (885647 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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