Vibrio phage nt-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Schizotequatrovirus; Vibrio virus nt1

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9TIC4|R9TIC4_9CAUD Uncharacterized protein OS=Vibrio phage nt-1 OX=115992 GN=VPFG_00113 PE=4 SV=1
MM1 pKa = 7.5AKK3 pKa = 10.14LVEE6 pKa = 4.71VYY8 pKa = 8.27STPAKK13 pKa = 9.7KK14 pKa = 10.78VNIAGVEE21 pKa = 3.92LVVEE25 pKa = 4.05NRR27 pKa = 11.84FNYY30 pKa = 8.03VTIDD34 pKa = 3.2QSGTVWAYY42 pKa = 9.88EE43 pKa = 4.3DD44 pKa = 4.98SPSLCNDD51 pKa = 2.86GDD53 pKa = 3.94GAYY56 pKa = 9.25WDD58 pKa = 3.84VWSGEE63 pKa = 4.2SDD65 pKa = 4.17GIAQFSDD72 pKa = 3.3VTEE75 pKa = 4.15EE76 pKa = 4.26EE77 pKa = 4.12FTKK80 pKa = 10.62LYY82 pKa = 10.58EE83 pKa = 4.09VV84 pKa = 3.99

Molecular weight: 9.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9TJ23|R9TJ23_9CAUD Uncharacterized protein OS=Vibrio phage nt-1 OX=115992 GN=VPFG_00090 PE=4 SV=1
MM1 pKa = 7.57GKK3 pKa = 8.26TYY5 pKa = 10.78TDD7 pKa = 3.01IAKK10 pKa = 9.76HH11 pKa = 4.37YY12 pKa = 8.35QRR14 pKa = 11.84KK15 pKa = 9.83GEE17 pKa = 5.3DD18 pKa = 3.3IPHH21 pKa = 6.72WLAKK25 pKa = 9.64KK26 pKa = 10.53LKK28 pKa = 8.86RR29 pKa = 11.84DD30 pKa = 3.52QHH32 pKa = 5.57GLCAGFRR39 pKa = 11.84WGNSRR44 pKa = 11.84RR45 pKa = 11.84SKK47 pKa = 11.24AKK49 pKa = 10.23DD50 pKa = 3.24KK51 pKa = 11.23QITTQIEE58 pKa = 3.9RR59 pKa = 11.84SQLKK63 pKa = 9.73RR64 pKa = 11.84KK65 pKa = 10.09RR66 pKa = 11.84GMII69 pKa = 3.79

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

405

0

405

75939

29

1246

187.5

21.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.884 ± 0.141

1.251 ± 0.067

7.012 ± 0.114

7.463 ± 0.158

4.347 ± 0.091

6.001 ± 0.175

2.352 ± 0.074

6.62 ± 0.122

6.574 ± 0.194

7.555 ± 0.102

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.094

5.119 ± 0.122

2.964 ± 0.08

3.315 ± 0.071

4.921 ± 0.085

6.285 ± 0.135

6.135 ± 0.186

6.869 ± 0.107

1.393 ± 0.055

4.195 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski