Hubei virga-like virus 11

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJP3|A0A1L3KJP3_9VIRU Uncharacterized protein OS=Hubei virga-like virus 11 OX=1923326 PE=4 SV=1
MM1 pKa = 7.98PYY3 pKa = 9.07TWTTEE8 pKa = 3.71ALLLTTHH15 pKa = 6.38HH16 pKa = 5.77WVEE19 pKa = 4.14YY20 pKa = 9.25AAFMRR25 pKa = 11.84IIGSFKK31 pKa = 10.18FKK33 pKa = 10.48QFSVKK38 pKa = 10.13AVRR41 pKa = 11.84DD42 pKa = 3.82SMLTTLANLEE52 pKa = 4.06YY53 pKa = 8.91DD54 pKa = 3.59TPFNVTTRR62 pKa = 11.84FPEE65 pKa = 4.02DD66 pKa = 3.66KK67 pKa = 10.42IYY69 pKa = 10.54IDD71 pKa = 4.55LSHH74 pKa = 6.66SVPLKK79 pKa = 10.43ILQQLTKK86 pKa = 10.75ALDD89 pKa = 3.71FSDD92 pKa = 3.41RR93 pKa = 11.84QTEE96 pKa = 4.13KK97 pKa = 11.15GRR99 pKa = 11.84VTTDD103 pKa = 2.92QEE105 pKa = 4.29KK106 pKa = 10.79NRR108 pKa = 11.84THH110 pKa = 7.43SNFEE114 pKa = 3.98DD115 pKa = 3.49AKK117 pKa = 9.43VAFYY121 pKa = 9.62TAVDD125 pKa = 3.64QLIDD129 pKa = 4.59LIGPFPIEE137 pKa = 3.85AAHH140 pKa = 6.27VYY142 pKa = 10.03GVYY145 pKa = 9.96TRR147 pKa = 11.84TSFEE151 pKa = 3.96IEE153 pKa = 4.36KK154 pKa = 10.62GLVWAA159 pKa = 5.47

Molecular weight: 18.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJW5|A0A1L3KJW5_9VIRU Uncharacterized protein OS=Hubei virga-like virus 11 OX=1923326 PE=4 SV=1
MM1 pKa = 7.43SLVKK5 pKa = 10.64LQFANILLPLEE16 pKa = 4.49VSTSEE21 pKa = 3.55MDD23 pKa = 3.35QFKK26 pKa = 11.44LEE28 pKa = 4.8LINLLMTTFGSVNLFSSFTIFAVLSNNMKK57 pKa = 10.25LHH59 pKa = 6.75PLQLSIVDD67 pKa = 3.61HH68 pKa = 6.2TEE70 pKa = 3.91PGSIPMNRR78 pKa = 11.84EE79 pKa = 3.26VAEE82 pKa = 5.01FIAKK86 pKa = 8.32TFSDD90 pKa = 4.82RR91 pKa = 11.84ICRR94 pKa = 11.84TIGYY98 pKa = 9.34NIYY101 pKa = 8.93TLEE104 pKa = 4.23LAHH107 pKa = 7.15APINNQVDD115 pKa = 3.71DD116 pKa = 4.94DD117 pKa = 4.24FLSRR121 pKa = 11.84LSKK124 pKa = 11.06LNLSSSSSSDD134 pKa = 3.06SGNEE138 pKa = 3.91SFTLTIRR145 pKa = 11.84KK146 pKa = 8.77DD147 pKa = 3.31ASIGQIFDD155 pKa = 4.46SIINNAKK162 pKa = 10.34DD163 pKa = 4.02YY164 pKa = 11.55LPMKK168 pKa = 8.85TIKK171 pKa = 10.36DD172 pKa = 3.54ALKK175 pKa = 10.63ASVDD179 pKa = 3.56KK180 pKa = 10.03FTSPSNASATTTNGSRR196 pKa = 11.84ISRR199 pKa = 11.84SKK201 pKa = 11.18SDD203 pKa = 4.46DD204 pKa = 3.19SLKK207 pKa = 10.19RR208 pKa = 11.84DD209 pKa = 3.61KK210 pKa = 11.17EE211 pKa = 4.21STEE214 pKa = 3.61TDD216 pKa = 2.76KK217 pKa = 11.59RR218 pKa = 11.84MLGNMTASDD227 pKa = 4.65LLNAMSRR234 pKa = 11.84GCSNTCNITCPKK246 pKa = 10.07CEE248 pKa = 4.07YY249 pKa = 10.36IVVKK253 pKa = 10.96NNSTSNNGSITPARR267 pKa = 11.84KK268 pKa = 9.6SNNVSDD274 pKa = 4.26CLFYY278 pKa = 10.69TPQRR282 pKa = 11.84RR283 pKa = 11.84LTDD286 pKa = 3.54YY287 pKa = 7.45EE288 pKa = 4.42TKK290 pKa = 9.84FTVNYY295 pKa = 9.72NSQVYY300 pKa = 8.66NVDD303 pKa = 3.15MVYY306 pKa = 10.24MGMSEE311 pKa = 4.23RR312 pKa = 11.84NKK314 pKa = 9.94TIAFLDD320 pKa = 3.65VLLSLNNRR328 pKa = 11.84RR329 pKa = 11.84LFTNATEE336 pKa = 3.81NSSYY340 pKa = 10.81IKK342 pKa = 10.08RR343 pKa = 11.84HH344 pKa = 4.72YY345 pKa = 9.38KK346 pKa = 10.3RR347 pKa = 11.84NVPVLSTKK355 pKa = 10.42SSNATITTRR364 pKa = 11.84DD365 pKa = 3.67PNSIAMLRR373 pKa = 11.84RR374 pKa = 11.84VTIPPPSTPRR384 pKa = 11.84PLINTFVDD392 pKa = 3.44SFDD395 pKa = 3.49KK396 pKa = 11.68VEE398 pKa = 4.2INPRR402 pKa = 11.84THH404 pKa = 6.96SNIIPYY410 pKa = 9.86PSVNTGVINVRR421 pKa = 11.84NDD423 pKa = 3.49ALKK426 pKa = 10.5KK427 pKa = 10.29IYY429 pKa = 10.07DD430 pKa = 3.86SNGTLIMDD438 pKa = 4.21FTNITLEE445 pKa = 4.28IIRR448 pKa = 11.84EE449 pKa = 3.91GHH451 pKa = 5.06TYY453 pKa = 11.08KK454 pKa = 9.98FTTAKK459 pKa = 10.39GSYY462 pKa = 7.91NTAGEE467 pKa = 4.05FMKK470 pKa = 10.99YY471 pKa = 9.88IVDD474 pKa = 4.09AYY476 pKa = 10.45GLRR479 pKa = 11.84QTLEE483 pKa = 3.74LFMDD487 pKa = 4.62KK488 pKa = 10.52FIDD491 pKa = 3.83MPDD494 pKa = 3.24TDD496 pKa = 4.96FQALLDD502 pKa = 4.63GIIKK506 pKa = 8.89RR507 pKa = 11.84TRR509 pKa = 11.84LMKK512 pKa = 10.66RR513 pKa = 11.84MMHH516 pKa = 6.02NDD518 pKa = 3.04IYY520 pKa = 10.94RR521 pKa = 11.84DD522 pKa = 3.54AITPVFLEE530 pKa = 4.3SFFTINKK537 pKa = 9.68DD538 pKa = 3.41DD539 pKa = 4.66LKK541 pKa = 10.68PLLSRR546 pKa = 11.84MVNSFNKK553 pKa = 10.25YY554 pKa = 7.53SDD556 pKa = 2.61KK557 pKa = 11.28GYY559 pKa = 10.37FVNNFIPLKK568 pKa = 9.87TYY570 pKa = 10.96YY571 pKa = 10.05EE572 pKa = 4.0YY573 pKa = 11.05SAYY576 pKa = 9.84KK577 pKa = 9.44DD578 pKa = 3.64TIKK581 pKa = 11.14SLVCKK586 pKa = 9.31TADD589 pKa = 3.3KK590 pKa = 11.0QQ591 pKa = 3.61

Molecular weight: 66.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1952

137

1065

488.0

56.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.996 ± 0.873

1.178 ± 0.283

6.762 ± 0.302

4.918 ± 0.721

5.328 ± 0.362

3.535 ± 0.579

2.049 ± 0.564

7.582 ± 0.409

7.889 ± 1.052

9.939 ± 0.693

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.561 ± 0.432

6.506 ± 1.121

3.535 ± 0.316

2.613 ± 0.46

4.355 ± 0.436

8.299 ± 0.986

6.814 ± 1.36

6.148 ± 0.581

0.666 ± 0.357

5.328 ± 0.577
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski