Porcine associated porprismacovirus 3
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|N0ACH6|N0ACH6_9VIRU Rep protein OS=Porcine associated porprismacovirus 3 OX=2170119 PE=4 SV=1
MM1 pKa = 7.34 KK2 pKa = 10.63 VKK4 pKa = 9.23 FTQFFDD10 pKa = 3.19 ISSSSEE16 pKa = 3.51 GFTTFKK22 pKa = 10.83 VVAGGDD28 pKa = 3.83 YY29 pKa = 10.71 VKK31 pKa = 10.32 QRR33 pKa = 11.84 CMPQFLCYY41 pKa = 9.81 KK42 pKa = 8.85 YY43 pKa = 10.6 YY44 pKa = 10.99 KK45 pKa = 10.07 LGPVSVKK52 pKa = 10.28 LVPASTLPVDD62 pKa = 3.88 PTGLSYY68 pKa = 10.46 ATGEE72 pKa = 4.23 NTVDD76 pKa = 3.47 PRR78 pKa = 11.84 DD79 pKa = 3.66 MFNPGLVRR87 pKa = 11.84 ITNGEE92 pKa = 3.93 FVDD95 pKa = 4.76 TVSSMDD101 pKa = 3.23 TYY103 pKa = 11.41 YY104 pKa = 10.65 ATLLDD109 pKa = 3.84 PRR111 pKa = 11.84 WFKK114 pKa = 10.86 FQLQQGFSRR123 pKa = 11.84 YY124 pKa = 9.42 ARR126 pKa = 11.84 PLVYY130 pKa = 10.62 NIGEE134 pKa = 4.04 IHH136 pKa = 6.49 QDD138 pKa = 3.37 PVGLTGQSIPDD149 pKa = 3.46 DD150 pKa = 3.88 SGINSGFNYY159 pKa = 10.32 YY160 pKa = 10.29 DD161 pKa = 3.4 VSGQKK166 pKa = 10.45 LKK168 pKa = 10.88 LDD170 pKa = 3.89 SYY172 pKa = 10.13 STNNLGNAISYY183 pKa = 10.64 SPIQTGRR190 pKa = 11.84 IKK192 pKa = 10.6 LGWMPTDD199 pKa = 3.22 TPTFTNGTQAGGLLSFVPEE218 pKa = 3.74 VDD220 pKa = 3.47 VITFVLPKK228 pKa = 10.27 AFKK231 pKa = 8.69 TKK233 pKa = 9.12 FYY235 pKa = 10.48 YY236 pKa = 10.06 RR237 pKa = 11.84 VFVEE241 pKa = 3.98 STVYY245 pKa = 10.58 FKK247 pKa = 11.29 DD248 pKa = 3.39 PVAINPIVAANGKK261 pKa = 8.88 VAFPMDD267 pKa = 3.88 RR268 pKa = 11.84 FVGLQAVNGGSLSQTWTNAQLGYY291 pKa = 10.35 HH292 pKa = 5.38 YY293 pKa = 10.59 KK294 pKa = 10.64 EE295 pKa = 4.33 EE296 pKa = 4.27 NEE298 pKa = 4.11 PP299 pKa = 3.65
Molecular weight: 33.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.219
IPC2_protein 6.3
IPC_protein 6.62
Toseland 6.62
ProMoST 7.073
Dawson 7.044
Bjellqvist 6.737
Wikipedia 7.029
Rodwell 7.015
Grimsley 6.678
Solomon 7.132
Lehninger 7.132
Nozaki 7.059
DTASelect 7.263
Thurlkill 7.278
EMBOSS 7.38
Sillero 7.351
Patrickios 3.745
IPC_peptide 7.146
IPC2_peptide 6.737
IPC2.peptide.svr19 6.876
Protein with the highest isoelectric point:
>tr|N0ACH6|N0ACH6_9VIRU Rep protein OS=Porcine associated porprismacovirus 3 OX=2170119 PE=4 SV=1
MM1 pKa = 7.31 SNKK4 pKa = 9.62 VYY6 pKa = 9.98 MATIPHH12 pKa = 5.92 SGITKK17 pKa = 9.51 RR18 pKa = 11.84 AFFKK22 pKa = 10.21 MIRR25 pKa = 11.84 DD26 pKa = 3.89 FDD28 pKa = 3.54 IHH30 pKa = 6.45 KK31 pKa = 9.56 WVYY34 pKa = 9.99 AVEE37 pKa = 4.48 RR38 pKa = 11.84 GHH40 pKa = 6.81 GGYY43 pKa = 9.96 KK44 pKa = 10.28 HH45 pKa = 5.34 IQCRR49 pKa = 11.84 FRR51 pKa = 11.84 TNKK54 pKa = 9.93 SFDD57 pKa = 3.87 QIRR60 pKa = 11.84 HH61 pKa = 5.26 ALICGHH67 pKa = 6.57 IEE69 pKa = 3.82 EE70 pKa = 6.08 ASDD73 pKa = 2.92 QWEE76 pKa = 4.26 YY77 pKa = 11.09 EE78 pKa = 4.33 KK79 pKa = 11.2 KK80 pKa = 10.35 DD81 pKa = 3.61 GNYY84 pKa = 6.89 MTSEE88 pKa = 4.37 DD89 pKa = 3.33 NHH91 pKa = 6.8 EE92 pKa = 4.13 ILKK95 pKa = 10.28 LRR97 pKa = 11.84 YY98 pKa = 8.8 GKK100 pKa = 7.78 PTKK103 pKa = 8.8 VQEE106 pKa = 3.83 WAMTLLEE113 pKa = 4.29 STNDD117 pKa = 3.34 RR118 pKa = 11.84 EE119 pKa = 4.41 VVVWVDD125 pKa = 2.89 KK126 pKa = 11.16 DD127 pKa = 3.9 GNSGKK132 pKa = 7.88 TWLTAHH138 pKa = 6.28 LWEE141 pKa = 5.42 RR142 pKa = 11.84 GQACYY147 pKa = 10.34 CPPTLSTPKK156 pKa = 10.39 EE157 pKa = 4.13 LISWVHH163 pKa = 5.19 SAYY166 pKa = 10.47 DD167 pKa = 3.47 QEE169 pKa = 4.45 PYY171 pKa = 10.35 IIIDD175 pKa = 4.36 IPRR178 pKa = 11.84 TWKK181 pKa = 9.93 WDD183 pKa = 3.57 DD184 pKa = 3.52 ALYY187 pKa = 9.47 TAIEE191 pKa = 4.37 TIKK194 pKa = 10.95 DD195 pKa = 3.31 GLVYY199 pKa = 10.39 DD200 pKa = 4.44 PRR202 pKa = 11.84 YY203 pKa = 8.29 SAHH206 pKa = 5.72 MRR208 pKa = 11.84 NIRR211 pKa = 11.84 GVKK214 pKa = 8.4 VMCMTNTEE222 pKa = 4.24 PKK224 pKa = 10.19 LSKK227 pKa = 10.38 LSEE230 pKa = 4.55 DD231 pKa = 2.85 RR232 pKa = 11.84 WVMYY236 pKa = 10.33 RR237 pKa = 11.84 PSNLFEE243 pKa = 3.96 TAEE246 pKa = 3.82 LRR248 pKa = 11.84 NRR250 pKa = 11.84 GGTPPRR256 pKa = 11.84 RR257 pKa = 11.84 SSGCNRR263 pKa = 11.84 SNLSPPVRR271 pKa = 4.44
Molecular weight: 31.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.959
IPC2_protein 8.083
IPC_protein 7.995
Toseland 8.039
ProMoST 8.624
Dawson 8.726
Bjellqvist 8.902
Wikipedia 8.741
Rodwell 8.785
Grimsley 7.98
Solomon 8.858
Lehninger 8.843
Nozaki 9.033
DTASelect 8.697
Thurlkill 8.77
EMBOSS 8.916
Sillero 9.033
Patrickios 4.431
IPC_peptide 8.858
IPC2_peptide 7.761
IPC2.peptide.svr19 7.827
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
570
271
299
285.0
32.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.737 ± 0.043
1.404 ± 0.577
5.789 ± 0.082
4.737 ± 1.357
4.737 ± 1.797
7.193 ± 1.181
2.105 ± 1.129
5.088 ± 0.845
6.316 ± 0.495
6.491 ± 0.418
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.632 ± 0.491
4.737 ± 0.22
5.965 ± 0.569
3.509 ± 0.922
5.263 ± 1.771
7.018 ± 0.268
7.368 ± 0.517
7.193 ± 1.444
2.281 ± 1.004
5.439 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here