Hubei diptera virus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phasivirus; Dipteran phasivirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KPJ1|A0A1L3KPJ1_9VIRU Replicase OS=Hubei diptera virus 5 OX=1922886 PE=4 SV=1
MM1 pKa = 7.05YY2 pKa = 10.34RR3 pKa = 11.84FVDD6 pKa = 5.27GIKK9 pKa = 9.92EE10 pKa = 4.05YY11 pKa = 10.64LWSIVLNTTWVSAGLIKK28 pKa = 10.4DD29 pKa = 4.02YY30 pKa = 10.63QISWILDD37 pKa = 3.32QLKK40 pKa = 10.71DD41 pKa = 3.76KK42 pKa = 11.0EE43 pKa = 4.45LTTEE47 pKa = 4.71LLHH50 pKa = 6.69IDD52 pKa = 3.86CSSVQVFALFLYY64 pKa = 9.48DD65 pKa = 3.46VCLQVAKK72 pKa = 10.54VYY74 pKa = 10.76QLDD77 pKa = 4.04TEE79 pKa = 4.95LIRR82 pKa = 11.84YY83 pKa = 8.4PDD85 pKa = 4.75DD86 pKa = 3.7EE87 pKa = 4.46SADD90 pKa = 3.61YY91 pKa = 11.3SLARR95 pKa = 11.84LLTYY99 pKa = 9.61FYY101 pKa = 10.37THH103 pKa = 6.94LHH105 pKa = 6.84PILTVGLEE113 pKa = 4.32VEE115 pKa = 5.2DD116 pKa = 4.08STSTTGLVVQEE127 pKa = 4.77DD128 pKa = 4.31KK129 pKa = 11.16GTLTLFTGPSTSSRR143 pKa = 11.84IPVRR147 pKa = 11.84TSASKK152 pKa = 8.21PQQMSRR158 pKa = 11.84EE159 pKa = 4.14MLGLTDD165 pKa = 3.5WLRR168 pKa = 11.84PEE170 pKa = 4.27VATQAVTSEE179 pKa = 3.78VMAAYY184 pKa = 7.62EE185 pKa = 4.08TMEE188 pKa = 4.61KK189 pKa = 10.24SLCEE193 pKa = 4.05SGLTDD198 pKa = 3.63EE199 pKa = 5.6AIKK202 pKa = 9.6GTADD206 pKa = 3.02YY207 pKa = 9.94FAYY210 pKa = 10.72VGFNANIIRR219 pKa = 11.84AALVSCEE226 pKa = 3.44ADD228 pKa = 3.07EE229 pKa = 4.37RR230 pKa = 11.84KK231 pKa = 9.89RR232 pKa = 11.84SIEE235 pKa = 3.62ICTLIVTVLSRR246 pKa = 11.84GTSIAATGKK255 pKa = 10.69EE256 pKa = 4.07KK257 pKa = 10.91MNDD260 pKa = 2.93TAKK263 pKa = 11.29AMFATLCSKK272 pKa = 10.61YY273 pKa = 10.1RR274 pKa = 11.84IKK276 pKa = 10.42RR277 pKa = 11.84TLTAKK282 pKa = 10.28RR283 pKa = 11.84HH284 pKa = 5.58LGPNDD289 pKa = 3.21VTLSRR294 pKa = 11.84VVAAHH299 pKa = 6.51PEE301 pKa = 3.8IAYY304 pKa = 9.25KK305 pKa = 10.76ALLCRR310 pKa = 11.84DD311 pKa = 4.07LEE313 pKa = 4.28RR314 pKa = 11.84PIRR317 pKa = 11.84VQTMINTGFVDD328 pKa = 5.35FKK330 pKa = 11.23AGLRR334 pKa = 11.84GSYY337 pKa = 9.88VFSVVPTNQKK347 pKa = 10.21GIKK350 pKa = 9.59HH351 pKa = 6.03SGAAAAALTYY361 pKa = 10.05MAMEE365 pKa = 4.45TVLLRR370 pKa = 11.84DD371 pKa = 3.62KK372 pKa = 10.57NAPAITLEE380 pKa = 4.08EE381 pKa = 4.29ALTNCATFARR391 pKa = 11.84ATLNSSRR398 pKa = 11.84YY399 pKa = 10.26DD400 pKa = 3.73DD401 pKa = 3.79DD402 pKa = 3.83TKK404 pKa = 11.35KK405 pKa = 10.59RR406 pKa = 11.84WFDD409 pKa = 3.2KK410 pKa = 10.55MDD412 pKa = 3.52VQEE415 pKa = 4.39EE416 pKa = 4.25TAKK419 pKa = 10.48AWIRR423 pKa = 11.84RR424 pKa = 11.84FSAKK428 pKa = 10.04FPEE431 pKa = 4.72GLDD434 pKa = 3.57LLASLGFPTSVAA446 pKa = 3.48

Molecular weight:
49.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KPK7|A0A1L3KPK7_9VIRU Putative glycoprotein OS=Hubei diptera virus 5 OX=1922886 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.98PLYY6 pKa = 10.13FRR8 pKa = 11.84ILIILVCRR16 pKa = 11.84VLLLFAVANNTSFGDD31 pKa = 3.69GSKK34 pKa = 10.02ILNIIQGGNFQTLNNHH50 pKa = 6.15GGFHH54 pKa = 6.69VKK56 pKa = 9.54TEE58 pKa = 4.08SDD60 pKa = 3.88KK61 pKa = 11.45INCPVCNCNNTEE73 pKa = 4.04LVNVIKK79 pKa = 9.93TFFEE83 pKa = 4.93SNNSSFKK90 pKa = 10.31TLNITNRR97 pKa = 11.84ATEE100 pKa = 4.02LTSNYY105 pKa = 8.7TLIKK109 pKa = 9.11MIPPSTSGNNDD120 pKa = 3.81DD121 pKa = 3.91NPKK124 pKa = 10.83AEE126 pKa = 4.53VEE128 pKa = 4.18LLSNTLNNQNQGSFIPPIMSFLVNRR153 pKa = 11.84IVHH156 pKa = 6.0EE157 pKa = 4.33EE158 pKa = 4.14PIEE161 pKa = 4.13MKK163 pKa = 10.32VLRR166 pKa = 11.84YY167 pKa = 8.63QDD169 pKa = 4.14KK170 pKa = 10.09IGQISEE176 pKa = 4.2QIKK179 pKa = 10.59DD180 pKa = 3.64IIFEE184 pKa = 4.25DD185 pKa = 3.97TSIVCDD191 pKa = 3.59TKK193 pKa = 11.03SLKK196 pKa = 10.59KK197 pKa = 9.95MLLCVSTYY205 pKa = 10.25IGEE208 pKa = 4.45DD209 pKa = 2.73QDD211 pKa = 4.86KK212 pKa = 10.25NIKK215 pKa = 8.64VLSMLLNLISDD226 pKa = 4.72FLSEE230 pKa = 4.47FKK232 pKa = 10.88LRR234 pKa = 11.84NKK236 pKa = 9.63NNNYY240 pKa = 10.04DD241 pKa = 3.84GNVKK245 pKa = 10.19LGKK248 pKa = 9.85FSHH251 pKa = 6.93LMDD254 pKa = 4.84QNDD257 pKa = 3.44KK258 pKa = 11.35SNAISSFGRR267 pKa = 11.84KK268 pKa = 8.91LLSVNKK274 pKa = 10.05DD275 pKa = 3.25VLFTSEE281 pKa = 4.49VEE283 pKa = 4.11PGTSIMRR290 pKa = 11.84SKK292 pKa = 10.37PDD294 pKa = 3.18EE295 pKa = 3.95YY296 pKa = 10.93DD297 pKa = 2.86ISVRR301 pKa = 11.84LSRR304 pKa = 11.84IMEE307 pKa = 4.27GPKK310 pKa = 9.63TSNIDD315 pKa = 3.1AVKK318 pKa = 10.03AFEE321 pKa = 4.14IFRR324 pKa = 11.84VHH326 pKa = 7.6CDD328 pKa = 3.01RR329 pKa = 11.84KK330 pKa = 10.23HH331 pKa = 6.6NDD333 pKa = 2.81VNRR336 pKa = 11.84LDD338 pKa = 4.64EE339 pKa = 5.18IEE341 pKa = 4.57LSAIVKK347 pKa = 8.41PHH349 pKa = 6.81FSTTTQVCFSTFLCPTDD366 pKa = 3.35YY367 pKa = 11.25HH368 pKa = 7.6FDD370 pKa = 3.44FTTRR374 pKa = 11.84EE375 pKa = 4.35CKK377 pKa = 9.94QSLDD381 pKa = 3.4GALLNDD387 pKa = 3.55EE388 pKa = 5.32FDD390 pKa = 4.02FKK392 pKa = 11.13SIKK395 pKa = 10.13LSSIVGRR402 pKa = 11.84VTSHH406 pKa = 6.67PFEE409 pKa = 4.59TIIPRR414 pKa = 11.84LKK416 pKa = 10.65KK417 pKa = 10.39NYY419 pKa = 9.86CKK421 pKa = 9.73IDD423 pKa = 3.09NHH425 pKa = 6.77SIRR428 pKa = 11.84KK429 pKa = 8.64CVGKK433 pKa = 10.22IKK435 pKa = 10.61KK436 pKa = 9.56FEE438 pKa = 4.32KK439 pKa = 10.55DD440 pKa = 3.2FMAIHH445 pKa = 6.43VANDD449 pKa = 3.26WVLVDD454 pKa = 4.47GDD456 pKa = 4.25YY457 pKa = 11.45LLTMVQDD464 pKa = 4.02SKK466 pKa = 11.7DD467 pKa = 3.42FRR469 pKa = 11.84SYY471 pKa = 11.0VCNSSNPLIIPDD483 pKa = 3.9TDD485 pKa = 3.41CRR487 pKa = 11.84GDD489 pKa = 3.43DD490 pKa = 3.44SFIRR494 pKa = 11.84AFGRR498 pKa = 11.84GNSDD502 pKa = 3.34CFCRR506 pKa = 11.84INNNWHH512 pKa = 6.72DD513 pKa = 4.26LVFKK517 pKa = 10.99NLDD520 pKa = 3.32GVSYY524 pKa = 10.42IDD526 pKa = 3.26AWIAFTWTVEE536 pKa = 3.65IDD538 pKa = 3.6SKK540 pKa = 11.49VIEE543 pKa = 4.69NLNCDD548 pKa = 3.66DD549 pKa = 6.62CEE551 pKa = 4.44FKK553 pKa = 10.91CKK555 pKa = 10.58NGIIDD560 pKa = 3.91YY561 pKa = 9.96SVKK564 pKa = 10.53KK565 pKa = 10.45YY566 pKa = 10.3ISLMKK571 pKa = 9.87ICFMDD576 pKa = 3.31HH577 pKa = 7.08CILRR581 pKa = 11.84DD582 pKa = 3.45IKK584 pKa = 11.13QMSGSIRR591 pKa = 11.84IPEE594 pKa = 3.8LRR596 pKa = 11.84FYY598 pKa = 11.14GSKK601 pKa = 10.61FKK603 pKa = 9.71ITLLSEE609 pKa = 4.2TKK611 pKa = 8.9TGEE614 pKa = 4.08FITEE618 pKa = 4.07VDD620 pKa = 3.95CSNIDD625 pKa = 3.41LCQSISCVFCLEE637 pKa = 3.89RR638 pKa = 11.84LANYY642 pKa = 9.25HH643 pKa = 6.34CLSSYY648 pKa = 11.35LLILYY653 pKa = 8.45VLILFATFLILSIIYY668 pKa = 10.12KK669 pKa = 9.96IMKK672 pKa = 8.92LLKK675 pKa = 10.24AIFMSLFVTLKK686 pKa = 10.29FSYY689 pKa = 10.72KK690 pKa = 9.36LVRR693 pKa = 11.84YY694 pKa = 9.58ICIKK698 pKa = 9.91FYY700 pKa = 10.41RR701 pKa = 11.84YY702 pKa = 9.74CRR704 pKa = 11.84GHH706 pKa = 5.93MSRR709 pKa = 11.84SYY711 pKa = 11.14NRR713 pKa = 11.84VVEE716 pKa = 5.79LDD718 pKa = 4.69DD719 pKa = 3.56QDD721 pKa = 4.03LWEE724 pKa = 4.63TPRR727 pKa = 11.84QDD729 pKa = 3.68EE730 pKa = 4.23EE731 pKa = 4.94SMEE734 pKa = 4.07DD735 pKa = 3.72VRR737 pKa = 11.84IDD739 pKa = 3.36DD740 pKa = 4.29HH741 pKa = 9.32SPMNRR746 pKa = 11.84TPMRR750 pKa = 11.84RR751 pKa = 11.84PRR753 pKa = 11.84VLPANRR759 pKa = 11.84TSRR762 pKa = 11.84DD763 pKa = 2.73IKK765 pKa = 9.79MSLFIVATIITLAFSCSDD783 pKa = 2.91IGSISTQDD791 pKa = 3.48VSCTEE796 pKa = 3.89YY797 pKa = 11.08KK798 pKa = 9.6NTISCVTDD806 pKa = 3.55FNIMLTVAPMGQYY819 pKa = 11.18ACIQAKK825 pKa = 10.1SIEE828 pKa = 4.1GRR830 pKa = 11.84VIMSIRR836 pKa = 11.84IKK838 pKa = 9.83TKK840 pKa = 10.2SIKK843 pKa = 10.26LICNKK848 pKa = 9.84RR849 pKa = 11.84SLYY852 pKa = 8.17YY853 pKa = 9.94TYY855 pKa = 9.79RR856 pKa = 11.84TTPTISKK863 pKa = 9.98VYY865 pKa = 9.48RR866 pKa = 11.84CDD868 pKa = 3.66SAGEE872 pKa = 4.05CSKK875 pKa = 10.94NVCNSNDD882 pKa = 3.17PKK884 pKa = 10.82IFEE887 pKa = 4.76KK888 pKa = 10.68EE889 pKa = 4.0LNPNEE894 pKa = 4.32LKK896 pKa = 11.13VLGNTGCKK904 pKa = 9.51SVNGFWGNGCFYY916 pKa = 10.92ASSACIFYY924 pKa = 10.55RR925 pKa = 11.84VLFPNVEE932 pKa = 3.92NGDD935 pKa = 3.82FYY937 pKa = 11.84EE938 pKa = 5.14MFDD941 pKa = 3.8CPHH944 pKa = 7.14WSWQIEE950 pKa = 4.15LEE952 pKa = 4.2ITQSDD957 pKa = 5.1LIQEE961 pKa = 4.44KK962 pKa = 10.06VSSVVLSNEE971 pKa = 4.14LPTQSSIGSIRR982 pKa = 11.84LHH984 pKa = 5.99SVSVPPSPVLSDD996 pKa = 3.95CFMSSSIAGMNFISLTNCNKK1016 pKa = 9.79KK1017 pKa = 10.33DD1018 pKa = 3.55QLIKK1022 pKa = 11.2GEE1024 pKa = 4.4LGEE1027 pKa = 4.22IQCPSGVDD1035 pKa = 3.19AGNATNKK1042 pKa = 9.81CSYY1045 pKa = 10.1SSSIYY1050 pKa = 10.81SVTASGNDD1058 pKa = 3.02IQFASEE1064 pKa = 4.27VINPKK1069 pKa = 8.96DD1070 pKa = 3.19TSKK1073 pKa = 11.38AKK1075 pKa = 10.33RR1076 pKa = 11.84LPLNFDD1082 pKa = 3.57GFMLDD1087 pKa = 3.79IDD1089 pKa = 3.86NSIPIARR1096 pKa = 11.84MFGSSLFQFSIATSKK1111 pKa = 11.26YY1112 pKa = 8.61KK1113 pKa = 10.77FEE1115 pKa = 4.11ILKK1118 pKa = 10.06EE1119 pKa = 3.97RR1120 pKa = 11.84SSCSTTFLNLTGCYY1134 pKa = 9.56NCLGGATLCLKK1145 pKa = 10.52VDD1147 pKa = 3.44VDD1149 pKa = 4.31KK1150 pKa = 11.17EE1151 pKa = 4.29SSILKK1156 pKa = 9.69VDD1158 pKa = 3.87CPKK1161 pKa = 11.16SKK1163 pKa = 10.2ISTVFMVNKK1172 pKa = 10.09NSRR1175 pKa = 11.84EE1176 pKa = 4.09YY1177 pKa = 10.68CSKK1180 pKa = 10.74LSSNVQTIHH1189 pKa = 6.14EE1190 pKa = 4.36EE1191 pKa = 4.3CEE1193 pKa = 4.26IVCSGNTEE1201 pKa = 4.18KK1202 pKa = 11.13VKK1204 pKa = 11.23LEE1206 pKa = 3.95ASLSYY1211 pKa = 10.62DD1212 pKa = 3.16VNVIHH1217 pKa = 7.6KK1218 pKa = 10.52DD1219 pKa = 3.18NTNYY1223 pKa = 10.07IYY1225 pKa = 10.98NGVGILRR1232 pKa = 11.84GVDD1235 pKa = 2.85ISTISLWSLGFIILGFLIFLIIILLIKK1262 pKa = 10.38KK1263 pKa = 9.93CLFDD1267 pKa = 4.44HH1268 pKa = 6.29KK1269 pKa = 8.84TLKK1272 pKa = 10.99AFDD1275 pKa = 4.78PSSKK1279 pKa = 10.72SII1281 pKa = 3.51

Molecular weight:
145.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3983

446

2256

1327.7

152.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.866 ± 1.72

2.234 ± 0.781

5.975 ± 0.154

6.528 ± 0.793

5.222 ± 0.453

4.168 ± 0.14

1.833 ± 0.179

8.285 ± 0.91

7.658 ± 0.545

9.691 ± 0.412

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.244

5.674 ± 0.988

3.163 ± 0.097

2.611 ± 0.175

4.62 ± 0.335

9.515 ± 0.684

5.85 ± 1.053

5.523 ± 0.524

1.054 ± 0.15

3.716 ± 0.245

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski