Hubei diptera virus 5
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KPJ1|A0A1L3KPJ1_9VIRU Replicase OS=Hubei diptera virus 5 OX=1922886 PE=4 SV=1
MM1 pKa = 7.05 YY2 pKa = 10.34 RR3 pKa = 11.84 FVDD6 pKa = 5.27 GIKK9 pKa = 9.92 EE10 pKa = 4.05 YY11 pKa = 10.64 LWSIVLNTTWVSAGLIKK28 pKa = 10.4 DD29 pKa = 4.02 YY30 pKa = 10.63 QISWILDD37 pKa = 3.32 QLKK40 pKa = 10.71 DD41 pKa = 3.76 KK42 pKa = 11.0 EE43 pKa = 4.45 LTTEE47 pKa = 4.71 LLHH50 pKa = 6.69 IDD52 pKa = 3.86 CSSVQVFALFLYY64 pKa = 9.48 DD65 pKa = 3.46 VCLQVAKK72 pKa = 10.54 VYY74 pKa = 10.76 QLDD77 pKa = 4.04 TEE79 pKa = 4.95 LIRR82 pKa = 11.84 YY83 pKa = 8.4 PDD85 pKa = 4.75 DD86 pKa = 3.7 EE87 pKa = 4.46 SADD90 pKa = 3.61 YY91 pKa = 11.3 SLARR95 pKa = 11.84 LLTYY99 pKa = 9.61 FYY101 pKa = 10.37 THH103 pKa = 6.94 LHH105 pKa = 6.84 PILTVGLEE113 pKa = 4.32 VEE115 pKa = 5.2 DD116 pKa = 4.08 STSTTGLVVQEE127 pKa = 4.77 DD128 pKa = 4.31 KK129 pKa = 11.16 GTLTLFTGPSTSSRR143 pKa = 11.84 IPVRR147 pKa = 11.84 TSASKK152 pKa = 8.21 PQQMSRR158 pKa = 11.84 EE159 pKa = 4.14 MLGLTDD165 pKa = 3.5 WLRR168 pKa = 11.84 PEE170 pKa = 4.27 VATQAVTSEE179 pKa = 3.78 VMAAYY184 pKa = 7.62 EE185 pKa = 4.08 TMEE188 pKa = 4.61 KK189 pKa = 10.24 SLCEE193 pKa = 4.05 SGLTDD198 pKa = 3.63 EE199 pKa = 5.6 AIKK202 pKa = 9.6 GTADD206 pKa = 3.02 YY207 pKa = 9.94 FAYY210 pKa = 10.72 VGFNANIIRR219 pKa = 11.84 AALVSCEE226 pKa = 3.44 ADD228 pKa = 3.07 EE229 pKa = 4.37 RR230 pKa = 11.84 KK231 pKa = 9.89 RR232 pKa = 11.84 SIEE235 pKa = 3.62 ICTLIVTVLSRR246 pKa = 11.84 GTSIAATGKK255 pKa = 10.69 EE256 pKa = 4.07 KK257 pKa = 10.91 MNDD260 pKa = 2.93 TAKK263 pKa = 11.29 AMFATLCSKK272 pKa = 10.61 YY273 pKa = 10.1 RR274 pKa = 11.84 IKK276 pKa = 10.42 RR277 pKa = 11.84 TLTAKK282 pKa = 10.28 RR283 pKa = 11.84 HH284 pKa = 5.58 LGPNDD289 pKa = 3.21 VTLSRR294 pKa = 11.84 VVAAHH299 pKa = 6.51 PEE301 pKa = 3.8 IAYY304 pKa = 9.25 KK305 pKa = 10.76 ALLCRR310 pKa = 11.84 DD311 pKa = 4.07 LEE313 pKa = 4.28 RR314 pKa = 11.84 PIRR317 pKa = 11.84 VQTMINTGFVDD328 pKa = 5.35 FKK330 pKa = 11.23 AGLRR334 pKa = 11.84 GSYY337 pKa = 9.88 VFSVVPTNQKK347 pKa = 10.21 GIKK350 pKa = 9.59 HH351 pKa = 6.03 SGAAAAALTYY361 pKa = 10.05 MAMEE365 pKa = 4.45 TVLLRR370 pKa = 11.84 DD371 pKa = 3.62 KK372 pKa = 10.57 NAPAITLEE380 pKa = 4.08 EE381 pKa = 4.29 ALTNCATFARR391 pKa = 11.84 ATLNSSRR398 pKa = 11.84 YY399 pKa = 10.26 DD400 pKa = 3.73 DD401 pKa = 3.79 DD402 pKa = 3.83 TKK404 pKa = 11.35 KK405 pKa = 10.59 RR406 pKa = 11.84 WFDD409 pKa = 3.2 KK410 pKa = 10.55 MDD412 pKa = 3.52 VQEE415 pKa = 4.39 EE416 pKa = 4.25 TAKK419 pKa = 10.48 AWIRR423 pKa = 11.84 RR424 pKa = 11.84 FSAKK428 pKa = 10.04 FPEE431 pKa = 4.72 GLDD434 pKa = 3.57 LLASLGFPTSVAA446 pKa = 3.48
Molecular weight: 49.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.788
IPC2_protein 5.881
IPC_protein 5.906
Toseland 6.109
ProMoST 6.224
Dawson 6.135
Bjellqvist 6.16
Wikipedia 6.135
Rodwell 6.122
Grimsley 6.198
Solomon 6.135
Lehninger 6.135
Nozaki 6.389
DTASelect 6.561
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.491
Patrickios 4.19
IPC_peptide 6.16
IPC2_peptide 6.415
IPC2.peptide.svr19 6.5
Protein with the highest isoelectric point:
>tr|A0A1L3KPK7|A0A1L3KPK7_9VIRU Putative glycoprotein OS=Hubei diptera virus 5 OX=1922886 PE=4 SV=1
MM1 pKa = 7.8 AKK3 pKa = 9.98 PLYY6 pKa = 10.13 FRR8 pKa = 11.84 ILIILVCRR16 pKa = 11.84 VLLLFAVANNTSFGDD31 pKa = 3.69 GSKK34 pKa = 10.02 ILNIIQGGNFQTLNNHH50 pKa = 6.15 GGFHH54 pKa = 6.69 VKK56 pKa = 9.54 TEE58 pKa = 4.08 SDD60 pKa = 3.88 KK61 pKa = 11.45 INCPVCNCNNTEE73 pKa = 4.04 LVNVIKK79 pKa = 9.93 TFFEE83 pKa = 4.93 SNNSSFKK90 pKa = 10.31 TLNITNRR97 pKa = 11.84 ATEE100 pKa = 4.02 LTSNYY105 pKa = 8.7 TLIKK109 pKa = 9.11 MIPPSTSGNNDD120 pKa = 3.81 DD121 pKa = 3.91 NPKK124 pKa = 10.83 AEE126 pKa = 4.53 VEE128 pKa = 4.18 LLSNTLNNQNQGSFIPPIMSFLVNRR153 pKa = 11.84 IVHH156 pKa = 6.0 EE157 pKa = 4.33 EE158 pKa = 4.14 PIEE161 pKa = 4.13 MKK163 pKa = 10.32 VLRR166 pKa = 11.84 YY167 pKa = 8.63 QDD169 pKa = 4.14 KK170 pKa = 10.09 IGQISEE176 pKa = 4.2 QIKK179 pKa = 10.59 DD180 pKa = 3.64 IIFEE184 pKa = 4.25 DD185 pKa = 3.97 TSIVCDD191 pKa = 3.59 TKK193 pKa = 11.03 SLKK196 pKa = 10.59 KK197 pKa = 9.95 MLLCVSTYY205 pKa = 10.25 IGEE208 pKa = 4.45 DD209 pKa = 2.73 QDD211 pKa = 4.86 KK212 pKa = 10.25 NIKK215 pKa = 8.64 VLSMLLNLISDD226 pKa = 4.72 FLSEE230 pKa = 4.47 FKK232 pKa = 10.88 LRR234 pKa = 11.84 NKK236 pKa = 9.63 NNNYY240 pKa = 10.04 DD241 pKa = 3.84 GNVKK245 pKa = 10.19 LGKK248 pKa = 9.85 FSHH251 pKa = 6.93 LMDD254 pKa = 4.84 QNDD257 pKa = 3.44 KK258 pKa = 11.35 SNAISSFGRR267 pKa = 11.84 KK268 pKa = 8.91 LLSVNKK274 pKa = 10.05 DD275 pKa = 3.25 VLFTSEE281 pKa = 4.49 VEE283 pKa = 4.11 PGTSIMRR290 pKa = 11.84 SKK292 pKa = 10.37 PDD294 pKa = 3.18 EE295 pKa = 3.95 YY296 pKa = 10.93 DD297 pKa = 2.86 ISVRR301 pKa = 11.84 LSRR304 pKa = 11.84 IMEE307 pKa = 4.27 GPKK310 pKa = 9.63 TSNIDD315 pKa = 3.1 AVKK318 pKa = 10.03 AFEE321 pKa = 4.14 IFRR324 pKa = 11.84 VHH326 pKa = 7.6 CDD328 pKa = 3.01 RR329 pKa = 11.84 KK330 pKa = 10.23 HH331 pKa = 6.6 NDD333 pKa = 2.81 VNRR336 pKa = 11.84 LDD338 pKa = 4.64 EE339 pKa = 5.18 IEE341 pKa = 4.57 LSAIVKK347 pKa = 8.41 PHH349 pKa = 6.81 FSTTTQVCFSTFLCPTDD366 pKa = 3.35 YY367 pKa = 11.25 HH368 pKa = 7.6 FDD370 pKa = 3.44 FTTRR374 pKa = 11.84 EE375 pKa = 4.35 CKK377 pKa = 9.94 QSLDD381 pKa = 3.4 GALLNDD387 pKa = 3.55 EE388 pKa = 5.32 FDD390 pKa = 4.02 FKK392 pKa = 11.13 SIKK395 pKa = 10.13 LSSIVGRR402 pKa = 11.84 VTSHH406 pKa = 6.67 PFEE409 pKa = 4.59 TIIPRR414 pKa = 11.84 LKK416 pKa = 10.65 KK417 pKa = 10.39 NYY419 pKa = 9.86 CKK421 pKa = 9.73 IDD423 pKa = 3.09 NHH425 pKa = 6.77 SIRR428 pKa = 11.84 KK429 pKa = 8.64 CVGKK433 pKa = 10.22 IKK435 pKa = 10.61 KK436 pKa = 9.56 FEE438 pKa = 4.32 KK439 pKa = 10.55 DD440 pKa = 3.2 FMAIHH445 pKa = 6.43 VANDD449 pKa = 3.26 WVLVDD454 pKa = 4.47 GDD456 pKa = 4.25 YY457 pKa = 11.45 LLTMVQDD464 pKa = 4.02 SKK466 pKa = 11.7 DD467 pKa = 3.42 FRR469 pKa = 11.84 SYY471 pKa = 11.0 VCNSSNPLIIPDD483 pKa = 3.9 TDD485 pKa = 3.41 CRR487 pKa = 11.84 GDD489 pKa = 3.43 DD490 pKa = 3.44 SFIRR494 pKa = 11.84 AFGRR498 pKa = 11.84 GNSDD502 pKa = 3.34 CFCRR506 pKa = 11.84 INNNWHH512 pKa = 6.72 DD513 pKa = 4.26 LVFKK517 pKa = 10.99 NLDD520 pKa = 3.32 GVSYY524 pKa = 10.42 IDD526 pKa = 3.26 AWIAFTWTVEE536 pKa = 3.65 IDD538 pKa = 3.6 SKK540 pKa = 11.49 VIEE543 pKa = 4.69 NLNCDD548 pKa = 3.66 DD549 pKa = 6.62 CEE551 pKa = 4.44 FKK553 pKa = 10.91 CKK555 pKa = 10.58 NGIIDD560 pKa = 3.91 YY561 pKa = 9.96 SVKK564 pKa = 10.53 KK565 pKa = 10.45 YY566 pKa = 10.3 ISLMKK571 pKa = 9.87 ICFMDD576 pKa = 3.31 HH577 pKa = 7.08 CILRR581 pKa = 11.84 DD582 pKa = 3.45 IKK584 pKa = 11.13 QMSGSIRR591 pKa = 11.84 IPEE594 pKa = 3.8 LRR596 pKa = 11.84 FYY598 pKa = 11.14 GSKK601 pKa = 10.61 FKK603 pKa = 9.71 ITLLSEE609 pKa = 4.2 TKK611 pKa = 8.9 TGEE614 pKa = 4.08 FITEE618 pKa = 4.07 VDD620 pKa = 3.95 CSNIDD625 pKa = 3.41 LCQSISCVFCLEE637 pKa = 3.89 RR638 pKa = 11.84 LANYY642 pKa = 9.25 HH643 pKa = 6.34 CLSSYY648 pKa = 11.35 LLILYY653 pKa = 8.45 VLILFATFLILSIIYY668 pKa = 10.12 KK669 pKa = 9.96 IMKK672 pKa = 8.92 LLKK675 pKa = 10.24 AIFMSLFVTLKK686 pKa = 10.29 FSYY689 pKa = 10.72 KK690 pKa = 9.36 LVRR693 pKa = 11.84 YY694 pKa = 9.58 ICIKK698 pKa = 9.91 FYY700 pKa = 10.41 RR701 pKa = 11.84 YY702 pKa = 9.74 CRR704 pKa = 11.84 GHH706 pKa = 5.93 MSRR709 pKa = 11.84 SYY711 pKa = 11.14 NRR713 pKa = 11.84 VVEE716 pKa = 5.79 LDD718 pKa = 4.69 DD719 pKa = 3.56 QDD721 pKa = 4.03 LWEE724 pKa = 4.63 TPRR727 pKa = 11.84 QDD729 pKa = 3.68 EE730 pKa = 4.23 EE731 pKa = 4.94 SMEE734 pKa = 4.07 DD735 pKa = 3.72 VRR737 pKa = 11.84 IDD739 pKa = 3.36 DD740 pKa = 4.29 HH741 pKa = 9.32 SPMNRR746 pKa = 11.84 TPMRR750 pKa = 11.84 RR751 pKa = 11.84 PRR753 pKa = 11.84 VLPANRR759 pKa = 11.84 TSRR762 pKa = 11.84 DD763 pKa = 2.73 IKK765 pKa = 9.79 MSLFIVATIITLAFSCSDD783 pKa = 2.91 IGSISTQDD791 pKa = 3.48 VSCTEE796 pKa = 3.89 YY797 pKa = 11.08 KK798 pKa = 9.6 NTISCVTDD806 pKa = 3.55 FNIMLTVAPMGQYY819 pKa = 11.18 ACIQAKK825 pKa = 10.1 SIEE828 pKa = 4.1 GRR830 pKa = 11.84 VIMSIRR836 pKa = 11.84 IKK838 pKa = 9.83 TKK840 pKa = 10.2 SIKK843 pKa = 10.26 LICNKK848 pKa = 9.84 RR849 pKa = 11.84 SLYY852 pKa = 8.17 YY853 pKa = 9.94 TYY855 pKa = 9.79 RR856 pKa = 11.84 TTPTISKK863 pKa = 9.98 VYY865 pKa = 9.48 RR866 pKa = 11.84 CDD868 pKa = 3.66 SAGEE872 pKa = 4.05 CSKK875 pKa = 10.94 NVCNSNDD882 pKa = 3.17 PKK884 pKa = 10.82 IFEE887 pKa = 4.76 KK888 pKa = 10.68 EE889 pKa = 4.0 LNPNEE894 pKa = 4.32 LKK896 pKa = 11.13 VLGNTGCKK904 pKa = 9.51 SVNGFWGNGCFYY916 pKa = 10.92 ASSACIFYY924 pKa = 10.55 RR925 pKa = 11.84 VLFPNVEE932 pKa = 3.92 NGDD935 pKa = 3.82 FYY937 pKa = 11.84 EE938 pKa = 5.14 MFDD941 pKa = 3.8 CPHH944 pKa = 7.14 WSWQIEE950 pKa = 4.15 LEE952 pKa = 4.2 ITQSDD957 pKa = 5.1 LIQEE961 pKa = 4.44 KK962 pKa = 10.06 VSSVVLSNEE971 pKa = 4.14 LPTQSSIGSIRR982 pKa = 11.84 LHH984 pKa = 5.99 SVSVPPSPVLSDD996 pKa = 3.95 CFMSSSIAGMNFISLTNCNKK1016 pKa = 9.79 KK1017 pKa = 10.33 DD1018 pKa = 3.55 QLIKK1022 pKa = 11.2 GEE1024 pKa = 4.4 LGEE1027 pKa = 4.22 IQCPSGVDD1035 pKa = 3.19 AGNATNKK1042 pKa = 9.81 CSYY1045 pKa = 10.1 SSSIYY1050 pKa = 10.81 SVTASGNDD1058 pKa = 3.02 IQFASEE1064 pKa = 4.27 VINPKK1069 pKa = 8.96 DD1070 pKa = 3.19 TSKK1073 pKa = 11.38 AKK1075 pKa = 10.33 RR1076 pKa = 11.84 LPLNFDD1082 pKa = 3.57 GFMLDD1087 pKa = 3.79 IDD1089 pKa = 3.86 NSIPIARR1096 pKa = 11.84 MFGSSLFQFSIATSKK1111 pKa = 11.26 YY1112 pKa = 8.61 KK1113 pKa = 10.77 FEE1115 pKa = 4.11 ILKK1118 pKa = 10.06 EE1119 pKa = 3.97 RR1120 pKa = 11.84 SSCSTTFLNLTGCYY1134 pKa = 9.56 NCLGGATLCLKK1145 pKa = 10.52 VDD1147 pKa = 3.44 VDD1149 pKa = 4.31 KK1150 pKa = 11.17 EE1151 pKa = 4.29 SSILKK1156 pKa = 9.69 VDD1158 pKa = 3.87 CPKK1161 pKa = 11.16 SKK1163 pKa = 10.2 ISTVFMVNKK1172 pKa = 10.09 NSRR1175 pKa = 11.84 EE1176 pKa = 4.09 YY1177 pKa = 10.68 CSKK1180 pKa = 10.74 LSSNVQTIHH1189 pKa = 6.14 EE1190 pKa = 4.36 EE1191 pKa = 4.3 CEE1193 pKa = 4.26 IVCSGNTEE1201 pKa = 4.18 KK1202 pKa = 11.13 VKK1204 pKa = 11.23 LEE1206 pKa = 3.95 ASLSYY1211 pKa = 10.62 DD1212 pKa = 3.16 VNVIHH1217 pKa = 7.6 KK1218 pKa = 10.52 DD1219 pKa = 3.18 NTNYY1223 pKa = 10.07 IYY1225 pKa = 10.98 NGVGILRR1232 pKa = 11.84 GVDD1235 pKa = 2.85 ISTISLWSLGFIILGFLIFLIIILLIKK1262 pKa = 10.38 KK1263 pKa = 9.93 CLFDD1267 pKa = 4.44 HH1268 pKa = 6.29 KK1269 pKa = 8.84 TLKK1272 pKa = 10.99 AFDD1275 pKa = 4.78 PSSKK1279 pKa = 10.72 SII1281 pKa = 3.51
Molecular weight: 145.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.648
IPC2_protein 6.898
IPC_protein 6.707
Toseland 6.453
ProMoST 7.337
Dawson 7.38
Bjellqvist 7.849
Wikipedia 7.293
Rodwell 7.395
Grimsley 6.478
Solomon 7.38
Lehninger 7.41
Nozaki 8.185
DTASelect 7.6
Thurlkill 7.644
EMBOSS 7.629
Sillero 7.966
Patrickios 3.999
IPC_peptide 7.38
IPC2_peptide 7.132
IPC2.peptide.svr19 6.994
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3983
446
2256
1327.7
152.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.866 ± 1.72
2.234 ± 0.781
5.975 ± 0.154
6.528 ± 0.793
5.222 ± 0.453
4.168 ± 0.14
1.833 ± 0.179
8.285 ± 0.91
7.658 ± 0.545
9.691 ± 0.412
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.812 ± 0.244
5.674 ± 0.988
3.163 ± 0.097
2.611 ± 0.175
4.62 ± 0.335
9.515 ± 0.684
5.85 ± 1.053
5.523 ± 0.524
1.054 ± 0.15
3.716 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here