Mycobacterium phage Leston

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4APY6|A0A2R4APY6_9CAUD Uncharacterized protein OS=Mycobacterium phage Leston OX=2126952 GN=43 PE=4 SV=1
MM1 pKa = 7.91ADD3 pKa = 4.12HH4 pKa = 6.9YY5 pKa = 10.87CTGDD9 pKa = 3.61DD10 pKa = 3.5CWNCEE15 pKa = 3.7RR16 pKa = 11.84RR17 pKa = 11.84ISQAEE22 pKa = 4.05YY23 pKa = 10.81EE24 pKa = 4.17RR25 pKa = 11.84DD26 pKa = 3.66CYY28 pKa = 11.43GDD30 pKa = 3.99DD31 pKa = 5.58DD32 pKa = 4.37YY33 pKa = 11.93PEE35 pKa = 5.01YY36 pKa = 11.24YY37 pKa = 10.52DD38 pKa = 3.64GTT40 pKa = 4.07

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4APW3|A0A2R4APW3_9CAUD Uncharacterized protein OS=Mycobacterium phage Leston OX=2126952 GN=89 PE=4 SV=1
MM1 pKa = 7.8SSGEE5 pKa = 4.06RR6 pKa = 11.84NMVCWTTCPHH16 pKa = 6.1CGKK19 pKa = 10.32RR20 pKa = 11.84SYY22 pKa = 10.43TSRR25 pKa = 11.84KK26 pKa = 6.02EE27 pKa = 3.68AKK29 pKa = 9.33RR30 pKa = 11.84AVSRR34 pKa = 11.84FRR36 pKa = 11.84GDD38 pKa = 2.77RR39 pKa = 11.84GGHH42 pKa = 4.46MSPYY46 pKa = 9.36RR47 pKa = 11.84CHH49 pKa = 7.07ADD51 pKa = 3.16EE52 pKa = 4.51TRR54 pKa = 11.84WHH56 pKa = 6.28VGHH59 pKa = 7.4LHH61 pKa = 5.18TATRR65 pKa = 11.84RR66 pKa = 11.84GINYY70 pKa = 9.4NEE72 pKa = 4.74KK73 pKa = 10.4VNQQ76 pKa = 3.53

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

19033

40

1369

202.5

21.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.704 ± 0.477

1.067 ± 0.161

6.652 ± 0.211

5.643 ± 0.293

2.701 ± 0.154

8.779 ± 0.422

2.191 ± 0.19

4.088 ± 0.179

3.436 ± 0.183

7.991 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.111

2.895 ± 0.202

5.643 ± 0.276

3.42 ± 0.161

7.377 ± 0.386

4.802 ± 0.274

6.657 ± 0.202

7.655 ± 0.228

1.939 ± 0.114

2.207 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski