Sporosarcina sp. BI001-red

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Sporosarcina; unclassified Sporosarcina

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3366 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8YRS9|A0A3D8YRS9_9BACL YjcZ family sporulation protein OS=Sporosarcina sp. BI001-red OX=2282866 GN=DVB69_16675 PE=3 SV=1
MM1 pKa = 7.38HH2 pKa = 6.99VLFYY6 pKa = 9.78TKK8 pKa = 9.83PGCPLCDD15 pKa = 3.4EE16 pKa = 5.19AEE18 pKa = 4.06QMMQLIQEE26 pKa = 5.02DD27 pKa = 5.09YY28 pKa = 9.74PLTWTAVNIEE38 pKa = 4.05EE39 pKa = 5.4DD40 pKa = 4.02DD41 pKa = 4.2VKK43 pKa = 11.06HH44 pKa = 5.16EE45 pKa = 4.43QYY47 pKa = 11.55VFMIPVVEE55 pKa = 4.66KK56 pKa = 10.62DD57 pKa = 4.55DD58 pKa = 4.48EE59 pKa = 4.61VLCSGVISYY68 pKa = 11.18VDD70 pKa = 3.56LALLFKK76 pKa = 10.99SGVV79 pKa = 3.29

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8YX91|A0A3D8YX91_9BACL tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Sporosarcina sp. BI001-red OX=2282866 GN=mnmG PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.58RR12 pKa = 11.84AKK14 pKa = 9.45VHH16 pKa = 5.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3366

0

3366

1000288

21

1945

297.2

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.626 ± 0.047

0.627 ± 0.014

5.253 ± 0.035

7.347 ± 0.052

4.484 ± 0.036

7.131 ± 0.042

2.002 ± 0.023

7.368 ± 0.041

6.136 ± 0.036

9.927 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.019

3.872 ± 0.029

3.749 ± 0.042

3.668 ± 0.027

4.203 ± 0.034

6.27 ± 0.032

5.962 ± 0.031

7.324 ± 0.046

0.973 ± 0.013

3.181 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski