Tupaia glis polyomavirus 1
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1CJR4|A0A2S1CJR4_9POLY Uncharacterized protein OS=Tupaia glis polyomavirus 1 OX=2170402 PE=4 SV=1
MM1 pKa = 7.39 GGVLSALIEE10 pKa = 4.49 VIVLATEE17 pKa = 4.43 IAAEE21 pKa = 4.13 TGLTAEE27 pKa = 4.65 ALLTGEE33 pKa = 4.6 ALSALEE39 pKa = 4.51 AEE41 pKa = 5.12 VFSLMTIDD49 pKa = 5.04 GISGIEE55 pKa = 4.02 ALAQLGWSAEE65 pKa = 4.12 QFSTLSYY72 pKa = 10.13 IANTFSTAVGYY83 pKa = 10.73 GVMFQTVSGISSLISVGMRR102 pKa = 11.84 LGLEE106 pKa = 3.97 VSSVNRR112 pKa = 11.84 AKK114 pKa = 10.07 TEE116 pKa = 3.92 QEE118 pKa = 3.65 LTALFGNIAKK128 pKa = 9.98 VIHH131 pKa = 6.42 VNLSHH136 pKa = 7.24 HH137 pKa = 7.24 LDD139 pKa = 3.53 PLKK142 pKa = 10.54 WCDD145 pKa = 4.1 SIMANYY151 pKa = 9.29 PKK153 pKa = 10.2 EE154 pKa = 4.12 LEE156 pKa = 4.64 NIPLDD161 pKa = 3.87 LRR163 pKa = 11.84 SKK165 pKa = 10.62 LGLIIEE171 pKa = 4.46 RR172 pKa = 11.84 ARR174 pKa = 11.84 WVIQKK179 pKa = 8.38 TPTTLPSEE187 pKa = 4.19 EE188 pKa = 4.61 SGDD191 pKa = 3.4 IVQLYY196 pKa = 7.04 EE197 pKa = 4.21 APGGALQRR205 pKa = 11.84 VTPDD209 pKa = 2.59 WLLPLILRR217 pKa = 11.84 LNGSQEE223 pKa = 3.91 KK224 pKa = 9.24 TPLCNSIQGSQTT236 pKa = 2.98
Molecular weight: 25.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.733
IPC2_protein 4.736
IPC_protein 4.609
Toseland 4.495
ProMoST 4.698
Dawson 4.546
Bjellqvist 4.685
Wikipedia 4.368
Rodwell 4.469
Grimsley 4.406
Solomon 4.533
Lehninger 4.495
Nozaki 4.647
DTASelect 4.724
Thurlkill 4.482
EMBOSS 4.393
Sillero 4.724
Patrickios 3.923
IPC_peptide 4.546
IPC2_peptide 4.724
IPC2.peptide.svr19 4.66
Protein with the highest isoelectric point:
>tr|A0A2S1CJR5|A0A2S1CJR5_9POLY Large T antigen OS=Tupaia glis polyomavirus 1 OX=2170402 GN=731T PE=4 SV=1
MM1 pKa = 7.19 SQASCQLLAGRR12 pKa = 11.84 HH13 pKa = 5.13 LCSSSVLALSRR24 pKa = 11.84 HH25 pKa = 6.32 LIPWLAACPVPAVISSRR42 pKa = 11.84 TKK44 pKa = 10.14 EE45 pKa = 4.09 VATTPVTSHH54 pKa = 5.95 TKK56 pKa = 10.02 VSYY59 pKa = 10.84
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.087
IPC2_protein 8.639
IPC_protein 8.843
Toseland 9.092
ProMoST 9.341
Dawson 9.37
Bjellqvist 9.502
Wikipedia 9.414
Rodwell 9.458
Grimsley 8.916
Solomon 9.692
Lehninger 9.706
Nozaki 9.794
DTASelect 9.282
Thurlkill 9.37
EMBOSS 9.56
Sillero 9.633
Patrickios 7.468
IPC_peptide 9.677
IPC2_peptide 9.048
IPC2.peptide.svr19 8.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2531
59
731
281.2
31.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.294 ± 0.498
2.924 ± 0.497
3.991 ± 0.454
7.033 ± 0.239
3.951 ± 0.678
6.006 ± 0.731
2.173 ± 0.36
5.176 ± 0.527
6.559 ± 0.522
11.339 ± 0.788
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.315
3.674 ± 0.432
6.875 ± 0.727
4.425 ± 0.275
4.781 ± 0.387
7.665 ± 0.561
5.61 ± 0.741
5.531 ± 0.232
1.817 ± 0.528
2.726 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here