Escherichia phage PTXU04

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Xuquatrovirus; Escherichia virus PTXU04

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MU09|A0A482MU09_9CAUD Uncharacterized protein OS=Escherichia phage PTXU04 OX=2508206 GN=PTXU04_00058 PE=4 SV=1
MM1 pKa = 7.44SNEE4 pKa = 4.06PKK6 pKa = 10.59HH7 pKa = 7.0IDD9 pKa = 3.26LCVNPNGLGDD19 pKa = 3.93VLSDD23 pKa = 3.19MLSIVNSDD31 pKa = 3.09RR32 pKa = 11.84SQGVFAVLGVVDD44 pKa = 5.13GVQLVLAASDD54 pKa = 3.62VEE56 pKa = 4.64EE57 pKa = 4.38YY58 pKa = 10.25QVHH61 pKa = 6.95DD62 pKa = 4.45EE63 pKa = 4.08EE64 pKa = 6.78LEE66 pKa = 4.03IQLDD70 pKa = 3.79DD71 pKa = 3.87PVIFEE76 pKa = 3.95QLKK79 pKa = 8.93LTFHH83 pKa = 7.13

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MSP0|A0A482MSP0_9CAUD Uncharacterized protein OS=Escherichia phage PTXU04 OX=2508206 GN=PTXU04_00081 PE=4 SV=1
MM1 pKa = 7.76CPRR4 pKa = 11.84LTRR7 pKa = 11.84CVGYY11 pKa = 10.59HH12 pKa = 6.27PMATNKK18 pKa = 9.52PHH20 pKa = 6.99FRR22 pKa = 11.84LTTYY26 pKa = 10.93GKK28 pKa = 10.5LNPGDD33 pKa = 4.63LVWEE37 pKa = 4.2PRR39 pKa = 11.84WRR41 pKa = 11.84NLRR44 pKa = 11.84RR45 pKa = 11.84ALKK48 pKa = 10.39VKK50 pKa = 10.3AIKK53 pKa = 10.51GNRR56 pKa = 11.84NDD58 pKa = 3.36TFLTIKK64 pKa = 10.48FIYY67 pKa = 8.89GEE69 pKa = 3.92WMIRR73 pKa = 11.84QPTDD77 pKa = 2.43KK78 pKa = 10.87CFASWW83 pKa = 2.96

Molecular weight: 9.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

19704

38

2197

214.2

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.881 ± 0.614

1.025 ± 0.193

6.116 ± 0.228

5.811 ± 0.381

3.344 ± 0.177

7.313 ± 0.285

1.979 ± 0.186

4.887 ± 0.243

5.43 ± 0.36

7.313 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.03 ± 0.21

5.014 ± 0.227

4.314 ± 0.241

4.446 ± 0.372

5.227 ± 0.232

6.08 ± 0.417

6.242 ± 0.381

6.542 ± 0.242

1.396 ± 0.13

3.608 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski