Agrococcus pavilionensis RW1
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2776 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U1LP10|U1LP10_9MICO YCII domain-containing protein OS=Agrococcus pavilionensis RW1 OX=1330458 GN=L332_06755 PE=3 SV=1
MM1 pKa = 7.75 KK2 pKa = 9.14 FTKK5 pKa = 10.26 LGRR8 pKa = 11.84 AAVVAAAGALLLSSCAANEE27 pKa = 4.17 SGADD31 pKa = 3.32 EE32 pKa = 4.48 AAPEE36 pKa = 4.57 GSQAAGSSLSGDD48 pKa = 3.54 LAGAGASSMGAAQEE62 pKa = 3.7 AWIAGFQTEE71 pKa = 4.28 NPDD74 pKa = 3.33 VNVTYY79 pKa = 10.73 DD80 pKa = 3.62 PAGSGAGRR88 pKa = 11.84 EE89 pKa = 3.91 QFIAGGVSFAGSDD102 pKa = 3.23 AYY104 pKa = 11.28 LKK106 pKa = 10.88 DD107 pKa = 3.7 EE108 pKa = 4.21 EE109 pKa = 4.97 LEE111 pKa = 4.42 GEE113 pKa = 4.62 FGACVPDD120 pKa = 4.09 TLPVDD125 pKa = 4.04 LPVYY129 pKa = 10.01 ISPIAVIFNVEE140 pKa = 4.33 GVDD143 pKa = 4.07 SLNMDD148 pKa = 3.97 ASTIAKK154 pKa = 9.4 IFSGQITSWDD164 pKa = 3.56 DD165 pKa = 3.13 PAIAEE170 pKa = 4.13 QNEE173 pKa = 4.59 GVEE176 pKa = 4.76 LPATPIQPVHH186 pKa = 6.82 RR187 pKa = 11.84 SDD189 pKa = 5.61 DD190 pKa = 3.57 SGTTEE195 pKa = 4.6 NFTEE199 pKa = 4.28 YY200 pKa = 10.76 LAATAAADD208 pKa = 3.25 WTEE211 pKa = 4.32 GVFEE215 pKa = 4.24 TWPSSVYY222 pKa = 9.39 STSEE226 pKa = 3.97 GAQGTSGVVDD236 pKa = 3.88 AVSNGTGTIGYY247 pKa = 9.82 ADD249 pKa = 4.09 ASRR252 pKa = 11.84 AGDD255 pKa = 3.84 LGTVALKK262 pKa = 10.81 VGEE265 pKa = 4.28 EE266 pKa = 4.18 YY267 pKa = 11.43 VPFTADD273 pKa = 2.88 AAAAAVDD280 pKa = 3.58 ASPRR284 pKa = 11.84 VEE286 pKa = 4.08 GRR288 pKa = 11.84 HH289 pKa = 5.63 EE290 pKa = 4.02 GDD292 pKa = 2.97 IAVEE296 pKa = 4.21 LDD298 pKa = 3.18 RR299 pKa = 11.84 TTTEE303 pKa = 3.66 AGVYY307 pKa = 8.98 PLVLISYY314 pKa = 9.68 AIACSEE320 pKa = 4.16 YY321 pKa = 9.93 QDD323 pKa = 4.6 AEE325 pKa = 3.96 AGEE328 pKa = 4.31 LVKK331 pKa = 10.75 AYY333 pKa = 10.34 LSYY336 pKa = 10.75 VASEE340 pKa = 4.28 EE341 pKa = 4.17 GQAAGQEE348 pKa = 4.36 NAGNAPISDD357 pKa = 3.97 EE358 pKa = 4.24 LRR360 pKa = 11.84 DD361 pKa = 3.9 EE362 pKa = 4.14 ILAVVDD368 pKa = 4.98 SIQQ371 pKa = 3.27
Molecular weight: 37.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.579
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.592
Sillero 3.821
Patrickios 1.825
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|U1MNN2|U1MNN2_9MICO Uncharacterized protein OS=Agrococcus pavilionensis RW1 OX=1330458 GN=L332_03425 PE=4 SV=1
MM1 pKa = 7.56 NILSRR6 pKa = 11.84 LRR8 pKa = 11.84 SAVSGRR14 pKa = 11.84 RR15 pKa = 11.84 TGGAARR21 pKa = 11.84 GRR23 pKa = 11.84 RR24 pKa = 11.84 STGMGGAAGARR35 pKa = 11.84 GRR37 pKa = 11.84 GSLLSSALGWISGRR51 pKa = 11.84 NRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 3.11
Molecular weight: 5.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.31
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2776
0
2776
872884
29
2082
314.4
33.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.464 ± 0.067
0.475 ± 0.011
6.118 ± 0.044
6.472 ± 0.045
2.81 ± 0.03
9.062 ± 0.044
2.094 ± 0.023
4.343 ± 0.034
1.688 ± 0.026
10.343 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.9 ± 0.019
1.5 ± 0.023
5.43 ± 0.036
2.843 ± 0.025
8.008 ± 0.061
5.193 ± 0.031
5.286 ± 0.039
8.826 ± 0.044
1.506 ± 0.022
1.639 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here