Pseudescherichia vulneris NBRC 102420

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Pseudescherichia; Pseudescherichia vulneris

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090V1S9|A0A090V1S9_PSEVU Proton/glutamate symporter OS=Pseudescherichia vulneris NBRC 102420 OX=1115515 GN=gltP PE=4 SV=1
MM1 pKa = 7.96AINDD5 pKa = 4.44FKK7 pKa = 11.32PFATGEE13 pKa = 4.17NANVTNQADD22 pKa = 4.04WEE24 pKa = 4.17ALPALGSGFQSGTASSAQVNKK45 pKa = 10.6ALRR48 pKa = 11.84QATAMGSTMGQFIANAGSDD67 pKa = 3.57ALDD70 pKa = 3.93NGDD73 pKa = 3.46IATLVAQFTQALTTSLGLGTAAYY96 pKa = 10.36LEE98 pKa = 4.81TVTSATDD105 pKa = 3.8FSANRR110 pKa = 11.84VVTTGWMGLGGGLSIGSDD128 pKa = 3.44SEE130 pKa = 4.85AISGQQLFDD139 pKa = 4.25LLRR142 pKa = 11.84TNYY145 pKa = 9.73PGSCFIRR152 pKa = 11.84CNYY155 pKa = 9.29QSSDD159 pKa = 3.14SSFATDD165 pKa = 3.8SPGVWFVGGDD175 pKa = 3.23TCTYY179 pKa = 9.46IQSDD183 pKa = 3.8YY184 pKa = 10.8RR185 pKa = 11.84NGVVRR190 pKa = 11.84VLSGNMLGTLFEE202 pKa = 4.13NTLYY206 pKa = 9.44GTANPPPCVQNIQLGAMSEE225 pKa = 4.15MDD227 pKa = 3.93SPDD230 pKa = 4.18DD231 pKa = 4.0GSSRR235 pKa = 11.84QFFPPTGCVLTGLWIDD251 pKa = 4.2EE252 pKa = 4.63SPSGAADD259 pKa = 3.38NTVAIYY265 pKa = 9.85AKK267 pKa = 10.23PIQQLLNGTWVTISGG282 pKa = 3.7

Molecular weight: 29.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090V443|A0A090V443_PSEVU Putative oxidoreductase YbdR OS=Pseudescherichia vulneris NBRC 102420 OX=1115515 GN=ybdR PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4196

0

4196

1303881

33

3301

310.7

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.118 ± 0.043

1.006 ± 0.014

5.306 ± 0.029

5.665 ± 0.037

3.798 ± 0.025

7.493 ± 0.035

2.204 ± 0.021

5.607 ± 0.034

4.063 ± 0.034

10.801 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.018

3.683 ± 0.026

4.514 ± 0.026

4.479 ± 0.028

5.749 ± 0.036

5.865 ± 0.029

5.465 ± 0.034

7.161 ± 0.031

1.531 ± 0.016

2.779 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski