Thioalkalivibrio denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thioalkalivibrio

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3346 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V3NR97|A0A1V3NR97_9GAMM Uncharacterized protein OS=Thioalkalivibrio denitrificans OX=108003 GN=B1C78_03630 PE=4 SV=1
MM1 pKa = 7.39IKK3 pKa = 10.34KK4 pKa = 10.05LVVAAALGTLMSTGTAFGSAFQINVGYY31 pKa = 10.27DD32 pKa = 3.2ATGDD36 pKa = 3.61GSTLTGVIEE45 pKa = 4.03EE46 pKa = 4.6LGYY49 pKa = 9.56TGTRR53 pKa = 11.84ATSIYY58 pKa = 10.71LGDD61 pKa = 3.85PSVAGTQIIDD71 pKa = 3.69TNIDD75 pKa = 3.31SVMDD79 pKa = 3.64SYY81 pKa = 11.89GFSAGSHH88 pKa = 4.23TTVGGTTVSFNYY100 pKa = 10.13PLDD103 pKa = 4.16PQQKK107 pKa = 10.02NISALNFLGAGEE119 pKa = 4.77GNGFTDD125 pKa = 4.94GVIVPYY131 pKa = 10.62GATAPNDD138 pKa = 3.7GVFWGITYY146 pKa = 9.85DD147 pKa = 4.04YY148 pKa = 10.09NIQGQITATGLEE160 pKa = 4.35FTSGSFDD167 pKa = 3.47VYY169 pKa = 11.0FEE171 pKa = 4.62NGTDD175 pKa = 3.87RR176 pKa = 11.84IQVLRR181 pKa = 11.84LDD183 pKa = 3.88VTGSQLDD190 pKa = 3.69PANLYY195 pKa = 8.48LTGPISYY202 pKa = 10.12DD203 pKa = 3.14FAGADD208 pKa = 3.47DD209 pKa = 5.39AFVQNFFVDD218 pKa = 3.92VASGMTFYY226 pKa = 10.72EE227 pKa = 4.48LWAAGEE233 pKa = 4.07DD234 pKa = 3.6TKK236 pKa = 11.53LGINWILDD244 pKa = 3.71TNIDD248 pKa = 3.96PPIPTLDD255 pKa = 3.28QLVAFTYY262 pKa = 10.96DD263 pKa = 3.52DD264 pKa = 4.05DD265 pKa = 3.86QTALFRR271 pKa = 11.84QTNLDD276 pKa = 3.37GSVTFQVVPEE286 pKa = 4.1PMMLTLFGAGLLALGFAARR305 pKa = 11.84RR306 pKa = 11.84RR307 pKa = 11.84RR308 pKa = 11.84NQTT311 pKa = 2.68

Molecular weight: 33.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V3NJ10|A0A1V3NJ10_9GAMM Cell division protein BolA OS=Thioalkalivibrio denitrificans OX=108003 GN=B1C78_07935 PE=3 SV=1
MM1 pKa = 7.84AIKK4 pKa = 10.31KK5 pKa = 9.79AATKK9 pKa = 10.36KK10 pKa = 10.33KK11 pKa = 10.21VSTKK15 pKa = 10.47SRR17 pKa = 11.84LKK19 pKa = 9.78KK20 pKa = 9.28TSTKK24 pKa = 10.38KK25 pKa = 10.27KK26 pKa = 10.07AATKK30 pKa = 9.8KK31 pKa = 9.43KK32 pKa = 9.55AVRR35 pKa = 11.84RR36 pKa = 11.84AATKK40 pKa = 10.17KK41 pKa = 9.94KK42 pKa = 9.34AVKK45 pKa = 10.29RR46 pKa = 11.84KK47 pKa = 7.63VAKK50 pKa = 10.25KK51 pKa = 10.12KK52 pKa = 10.15AVKK55 pKa = 10.11KK56 pKa = 10.46KK57 pKa = 10.67AGAKK61 pKa = 9.42KK62 pKa = 9.96KK63 pKa = 9.66AVKK66 pKa = 10.35RR67 pKa = 11.84KK68 pKa = 7.63VAKK71 pKa = 10.25KK72 pKa = 10.12KK73 pKa = 10.15AVKK76 pKa = 10.11KK77 pKa = 10.46KK78 pKa = 10.67AGAKK82 pKa = 9.42KK83 pKa = 9.96KK84 pKa = 9.66AVKK87 pKa = 10.35RR88 pKa = 11.84KK89 pKa = 7.63VAKK92 pKa = 10.25KK93 pKa = 10.12KK94 pKa = 10.15AVKK97 pKa = 10.11KK98 pKa = 10.46KK99 pKa = 10.67AGAKK103 pKa = 9.48KK104 pKa = 10.18KK105 pKa = 10.06AVKK108 pKa = 10.08KK109 pKa = 8.95ATRR112 pKa = 11.84KK113 pKa = 8.34ATSKK117 pKa = 10.7KK118 pKa = 9.92KK119 pKa = 10.79ASTKK123 pKa = 10.19KK124 pKa = 9.59KK125 pKa = 8.44AAARR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84TTKK134 pKa = 10.44KK135 pKa = 10.23KK136 pKa = 10.52AA137 pKa = 3.08

Molecular weight: 15.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3346

0

3346

1015271

27

1994

303.4

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.932 ± 0.047

0.928 ± 0.016

5.969 ± 0.034

6.68 ± 0.04

3.443 ± 0.026

8.365 ± 0.041

2.567 ± 0.023

4.537 ± 0.033

2.376 ± 0.034

10.959 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.022

2.384 ± 0.019

5.236 ± 0.032

3.282 ± 0.021

8.429 ± 0.046

4.851 ± 0.03

4.863 ± 0.026

7.82 ± 0.036

1.414 ± 0.018

2.385 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski