Euphorbia mosaic Peru virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9JPY3|A9JPY3_9GEMI Replication-associated protein OS=Euphorbia mosaic Peru virus OX=2169733 GN=AC1 PE=3 SV=1
MM1 pKa = 7.5LNSSSSTPPSIKK13 pKa = 10.0AQHH16 pKa = 6.47KK17 pKa = 8.26IAKK20 pKa = 8.97KK21 pKa = 8.49RR22 pKa = 11.84AIRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84IDD30 pKa = 3.8LNCGCSIFLHH40 pKa = 6.68IDD42 pKa = 3.17CADD45 pKa = 3.34YY46 pKa = 10.92GFKK49 pKa = 10.49HH50 pKa = 6.48RR51 pKa = 11.84GVHH54 pKa = 5.6HH55 pKa = 6.41CVSGGEE61 pKa = 3.88FRR63 pKa = 11.84FYY65 pKa = 11.07LGGSKK70 pKa = 10.38SPLFQDD76 pKa = 3.38HH77 pKa = 6.07QGRR80 pKa = 11.84GPNLHH85 pKa = 6.19VSQDD89 pKa = 3.35IPHH92 pKa = 7.29PSPVQLQPQEE102 pKa = 4.46GTEE105 pKa = 4.27STQSFPEE112 pKa = 4.62LPSLDD117 pKa = 5.53DD118 pKa = 3.76IDD120 pKa = 4.94SSFWDD125 pKa = 4.24DD126 pKa = 3.84LFKK129 pKa = 11.26

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9JPY0|A9JPY0_9GEMI Isoform of A9JPY3 Capsid protein OS=Euphorbia mosaic Peru virus OX=2169733 GN=AC1 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.65RR4 pKa = 11.84DD5 pKa = 3.82APWRR9 pKa = 11.84LMAGTSKK16 pKa = 10.62VSRR19 pKa = 11.84NVNYY23 pKa = 10.36SPRR26 pKa = 11.84GGPKK30 pKa = 9.49LDD32 pKa = 4.58KK33 pKa = 10.77AAAWVNRR40 pKa = 11.84PMYY43 pKa = 9.63RR44 pKa = 11.84KK45 pKa = 8.95PRR47 pKa = 11.84IYY49 pKa = 9.54RR50 pKa = 11.84TLRR53 pKa = 11.84SPDD56 pKa = 3.35VPKK59 pKa = 10.93GCEE62 pKa = 4.39GPCKK66 pKa = 9.35VQSYY70 pKa = 6.87EE71 pKa = 3.74QRR73 pKa = 11.84HH74 pKa = 5.66DD75 pKa = 3.55VSHH78 pKa = 5.99VGKK81 pKa = 10.07VICISDD87 pKa = 3.51VTRR90 pKa = 11.84GNGITHH96 pKa = 6.78RR97 pKa = 11.84VGKK100 pKa = 9.44RR101 pKa = 11.84FCVKK105 pKa = 9.68SVYY108 pKa = 10.62VIGKK112 pKa = 8.45IWMDD116 pKa = 3.43EE117 pKa = 4.01NIKK120 pKa = 10.61LKK122 pKa = 10.73NHH124 pKa = 5.95TNSVMFWLVRR134 pKa = 11.84DD135 pKa = 3.7RR136 pKa = 11.84RR137 pKa = 11.84PYY139 pKa = 8.05GTPMDD144 pKa = 4.6FGQVFNMFDD153 pKa = 3.99NEE155 pKa = 4.11PSTATVKK162 pKa = 10.85NDD164 pKa = 2.82LRR166 pKa = 11.84DD167 pKa = 3.54RR168 pKa = 11.84FQVMHH173 pKa = 6.97KK174 pKa = 9.73FYY176 pKa = 11.33AKK178 pKa = 9.12VTGGQYY184 pKa = 11.07ASNEE188 pKa = 3.69QAIVKK193 pKa = 9.05RR194 pKa = 11.84FWKK197 pKa = 10.22VNNHH201 pKa = 4.48VVYY204 pKa = 10.67NHH206 pKa = 5.8QEE208 pKa = 3.47AGKK211 pKa = 10.41YY212 pKa = 8.39EE213 pKa = 4.09NHH215 pKa = 6.52TEE217 pKa = 3.99NALLLYY223 pKa = 7.29MACTHH228 pKa = 7.07ASNPVYY234 pKa = 9.86ATLKK238 pKa = 9.47IRR240 pKa = 11.84IYY242 pKa = 10.59FYY244 pKa = 11.38DD245 pKa = 4.2SITNN249 pKa = 3.6

Molecular weight: 28.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

1015

121

384

203.0

23.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.419 ± 1.201

1.773 ± 0.496

5.517 ± 0.723

3.448 ± 0.391

4.631 ± 0.662

5.616 ± 0.547

3.448 ± 0.539

6.01 ± 0.635

5.911 ± 0.888

6.897 ± 0.826

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.639

5.813 ± 0.817

6.207 ± 1.092

3.941 ± 0.444

6.798 ± 0.605

8.966 ± 1.883

5.222 ± 1.017

6.404 ± 1.275

1.576 ± 0.343

4.039 ± 0.927
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski