Coniella lustricola
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11309 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T3A7R7|A0A2T3A7R7_9PEZI Uncharacterized protein OS=Coniella lustricola OX=2025994 GN=BD289DRAFT_434149 PE=4 SV=1
MM1 pKa = 7.64 KK2 pKa = 9.48 FTGSRR7 pKa = 11.84 NGLAAAAAALAILQSQVAASPILRR31 pKa = 11.84 LHH33 pKa = 6.62 RR34 pKa = 11.84 KK35 pKa = 8.61 SATSIPPFAITPTSSILSSSLTSQPALSMPNGSPAASYY73 pKa = 10.8 VAFGLEE79 pKa = 3.89 IPITGITGTNPEE91 pKa = 4.13 QVFLGSEE98 pKa = 4.19 SYY100 pKa = 11.41 LLFVQQEE107 pKa = 4.28 MTLSADD113 pKa = 3.23 VSLNMSNNLLSSSVDD128 pKa = 3.85 PIVGFHH134 pKa = 6.74 ASSGFSSSSSSVSSSSSSISNSSAGSASDD163 pKa = 4.04 LSLLVVLVYY172 pKa = 11.08 NNQSTNATSSLNYY185 pKa = 10.2 LNQTSQSRR193 pKa = 11.84 QFDD196 pKa = 3.67 WTSWAASIAADD207 pKa = 3.29 SSLRR211 pKa = 11.84 MNFDD215 pKa = 3.04 MDD217 pKa = 4.17 TFASQAGLGKK227 pKa = 10.24 AVASEE232 pKa = 4.21 FVMITQDD239 pKa = 3.18 EE240 pKa = 4.73 SSGSGSASYY249 pKa = 10.88 SAVTTLSVGAGGASQTAAAVFLTTGLPSAAANGIPTMGAASAAVGVNGMASASLGSGSSAMATGAAAQGGYY320 pKa = 10.64 AFASAASGSSATGAAWASSSDD341 pKa = 3.64 SSSTSTDD348 pKa = 3.19 SNVGVAINNLVSAMSSSMSSIDD370 pKa = 3.79 SGVLNSDD377 pKa = 3.23 SSDD380 pKa = 3.63 NLGSAASGTASMAGGAAVALPTGFASTSLAGSTSSFASSNISSSTSSPTPGTCVAGIYY438 pKa = 9.9 KK439 pKa = 10.47 CSLDD443 pKa = 3.27 GSSYY447 pKa = 8.03 QQCASGGTWSVSMAMAAGTTCTPGVSSGLSISASPSTSGSLASSSGSATLSGLSSMDD504 pKa = 3.23 SSSDD508 pKa = 3.42 SSSSATASGMGLSGLGGLVSLKK530 pKa = 10.53 SSQPGSASASGMASEE545 pKa = 5.91 GNDD548 pKa = 3.2 ADD550 pKa = 4.1 IGSSDD555 pKa = 4.05 SILPKK560 pKa = 10.61 GSFSGASGMGSFEE573 pKa = 4.25 SSGNSAFSSGSSTLFHH589 pKa = 6.78 HH590 pKa = 7.16 GKK592 pKa = 10.36 GSSAGAKK599 pKa = 9.76 DD600 pKa = 3.43 SSSFASSGDD609 pKa = 3.59 SGSATSFLSGGSSSSGSGDD628 pKa = 3.18 LGSFSASDD636 pKa = 3.89 YY637 pKa = 11.05 SDD639 pKa = 3.31 SHH641 pKa = 7.29 PSFLMGGSASGASDD655 pKa = 3.45 GLGSLASAGEE665 pKa = 4.35 SASSIGSSSSSTQSGGSLSFLGGNSSDD692 pKa = 3.84 SQSGDD697 pKa = 3.35 SLSSVGVPVTATDD710 pKa = 3.65 SSISASSPEE719 pKa = 4.13 DD720 pKa = 3.3 SCDD723 pKa = 3.42 EE724 pKa = 4.62 DD725 pKa = 3.93 SATTTGSGDD734 pKa = 3.5 VSSSTSGSQGYY745 pKa = 9.76 DD746 pKa = 3.02 SPLSGGISMASGSASSASSASSSFGSSYY774 pKa = 10.42 GQSGASASSSTASSLAGSSYY794 pKa = 10.08 GQSGTSDD801 pKa = 3.05 SSLAASSSSSSAVIPYY817 pKa = 10.1 FGGSSASGSVDD828 pKa = 3.44 LSGLGDD834 pKa = 3.69 STVTDD839 pKa = 3.57 SSFGSSASSAYY850 pKa = 10.51 GNGASSGMALGGGSSSLSSASSSLGAGSTSVDD882 pKa = 3.4 TSSSGASISSVSGDD896 pKa = 3.64 SYY898 pKa = 11.39 STDD901 pKa = 2.96 ASSSGNASSSLSGDD915 pKa = 3.31 SSSIDD920 pKa = 3.3 ASSSGGDD927 pKa = 3.24 ASSSGSASSSLSGDD941 pKa = 3.31 SSSIDD946 pKa = 3.3 ASSSGGDD953 pKa = 3.24 ASSSGSASSSLSGDD967 pKa = 3.31 SSSIDD972 pKa = 3.3 ASSSGGDD979 pKa = 3.24 ASSSGSASSSLSGDD993 pKa = 3.41 SSSVDD998 pKa = 3.14 ASRR1001 pKa = 11.84 TGGDD1005 pKa = 3.46 ASSMSGDD1012 pKa = 3.47 SSIVDD1017 pKa = 3.61 ASSSGGDD1024 pKa = 3.03 ASSFGSAMSGASTGSGLEE1042 pKa = 4.42 DD1043 pKa = 3.97 GSSGGSGLGYY1053 pKa = 10.48 LSGSGALGASGTAQAQGAQSAMATGGYY1080 pKa = 9.18 QGSAFATAAYY1090 pKa = 9.94 QSVASGTASASAAVVTGAASNNLAAALGDD1119 pKa = 3.81 ALGNMAVTIGLDD1131 pKa = 2.93 IDD1133 pKa = 3.6 NGEE1136 pKa = 4.35 FEE1138 pKa = 4.56 TTITANLKK1146 pKa = 10.48 DD1147 pKa = 3.73 RR1148 pKa = 11.84 TVPTPSPVMWDD1159 pKa = 3.52 FRR1161 pKa = 11.84 KK1162 pKa = 10.62 GSLNARR1168 pKa = 11.84 ASPAPSSSSSSSSSSSSTVKK1188 pKa = 10.19 SAAAGRR1194 pKa = 11.84 VQEE1197 pKa = 4.83 AGPAKK1202 pKa = 10.01 QVVGVVFALCIASLVMM1218 pKa = 5.16
Molecular weight: 114.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.452
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.681
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.139
Thurlkill 3.516
EMBOSS 3.694
Sillero 3.834
Patrickios 1.392
IPC_peptide 3.706
IPC2_peptide 3.795
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|A0A2T2ZTW6|A0A2T2ZTW6_9PEZI Delta(24)-sterol reductase OS=Coniella lustricola OX=2025994 GN=BD289DRAFT_418248 PE=3 SV=1
MM1 pKa = 7.82 AGMLAMLSLVVRR13 pKa = 11.84 TLSWRR18 pKa = 11.84 PSQTHH23 pKa = 5.28 SAAGAAAAGEE33 pKa = 4.62 CATKK37 pKa = 8.46 TTLSSLHH44 pKa = 5.75 GRR46 pKa = 11.84 SLRR49 pKa = 11.84 RR50 pKa = 11.84 SHH52 pKa = 6.16 RR53 pKa = 11.84 QRR55 pKa = 11.84 HH56 pKa = 3.84 TRR58 pKa = 11.84 ARR60 pKa = 11.84 RR61 pKa = 11.84 PRR63 pKa = 11.84 RR64 pKa = 11.84 ATAQTVRR71 pKa = 11.84 HH72 pKa = 5.76 GWAAQQQQPPPQHH85 pKa = 6.68 PPRR88 pKa = 11.84 PRR90 pKa = 11.84 RR91 pKa = 11.84 PRR93 pKa = 11.84 TKK95 pKa = 7.53 TTRR98 pKa = 11.84 RR99 pKa = 11.84 TKK101 pKa = 8.27 TTTTSSCASACAAALAAAVAGPAMAAATTPKK132 pKa = 9.67 RR133 pKa = 11.84 AEE135 pKa = 4.01 RR136 pKa = 11.84 RR137 pKa = 11.84 RR138 pKa = 11.84 GAQSQSATSSRR149 pKa = 11.84 TTARR153 pKa = 11.84 GRR155 pKa = 11.84 RR156 pKa = 11.84 RR157 pKa = 11.84 CRR159 pKa = 11.84 RR160 pKa = 11.84 RR161 pKa = 11.84 RR162 pKa = 11.84 GAGG165 pKa = 3.04
Molecular weight: 17.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 10.789
IPC_protein 12.398
Toseland 12.559
ProMoST 13.056
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.12
Grimsley 12.588
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 11.828
IPC_peptide 13.056
IPC2_peptide 12.047
IPC2.peptide.svr19 9.158
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11309
0
11309
5000157
49
4995
442.1
48.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.45 ± 0.024
1.343 ± 0.01
5.675 ± 0.016
5.716 ± 0.024
3.562 ± 0.016
6.989 ± 0.021
2.517 ± 0.01
4.497 ± 0.015
4.498 ± 0.019
8.828 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.185 ± 0.009
3.54 ± 0.012
5.997 ± 0.025
4.194 ± 0.019
6.141 ± 0.019
8.448 ± 0.027
6.14 ± 0.017
6.148 ± 0.016
1.479 ± 0.009
2.651 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here