Peltaster fructicola
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8072 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H0XQ01|A0A6H0XQ01_9PEZI Phenylalanyl-tRNA synthetase OS=Peltaster fructicola OX=286661 GN=AMS68_002331 PE=3 SV=1
MM1 pKa = 7.8 DD2 pKa = 5.45 AEE4 pKa = 4.5 NIYY7 pKa = 11.08 DD8 pKa = 4.07 EE9 pKa = 5.5 IEE11 pKa = 4.47 IEE13 pKa = 5.6 DD14 pKa = 3.8 MTFDD18 pKa = 3.93 SALQMYY24 pKa = 8.48 HH25 pKa = 5.92 YY26 pKa = 8.49 PCPCGDD32 pKa = 3.16 RR33 pKa = 11.84 FEE35 pKa = 5.85 INIDD39 pKa = 3.58 DD40 pKa = 4.31 LRR42 pKa = 11.84 DD43 pKa = 3.65 GEE45 pKa = 4.73 EE46 pKa = 3.84 IAVCPSCSLMIRR58 pKa = 11.84 VIFDD62 pKa = 3.16 VDD64 pKa = 4.49 DD65 pKa = 4.13 LPKK68 pKa = 10.25 PDD70 pKa = 5.18 GGAQNAGQQAIATTVV85 pKa = 2.66
Molecular weight: 9.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.643
IPC_protein 3.605
Toseland 3.389
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.579
Rodwell 3.439
Grimsley 3.312
Solomon 3.592
Lehninger 3.541
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.465
EMBOSS 3.579
Sillero 3.732
Patrickios 0.477
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.715
Protein with the highest isoelectric point:
>tr|A0A6H0XSK2|A0A6H0XSK2_9PEZI C2H2-type domain-containing protein OS=Peltaster fructicola OX=286661 GN=AMS68_003231 PE=4 SV=1
MM1 pKa = 7.19 FRR3 pKa = 11.84 HH4 pKa = 5.99 ALGISKK10 pKa = 10.17 HH11 pKa = 5.43 IALPDD16 pKa = 3.33 VCFQCASIARR26 pKa = 11.84 PIAQQRR32 pKa = 11.84 SLQITARR39 pKa = 11.84 RR40 pKa = 11.84 QLASIRR46 pKa = 11.84 GQALDD51 pKa = 3.18 RR52 pKa = 11.84 SYY54 pKa = 11.15 FGSRR58 pKa = 11.84 SPDD61 pKa = 2.9 VSNAFRR67 pKa = 11.84 HH68 pKa = 4.48 TRR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 RR74 pKa = 11.84 KK75 pKa = 10.1 DD76 pKa = 3.14 EE77 pKa = 4.29 EE78 pKa = 4.24 KK79 pKa = 10.5 PLPLDD84 pKa = 3.81 ASSTLAQSASQAPARR99 pKa = 11.84 SIRR102 pKa = 11.84 RR103 pKa = 11.84 LISTYY108 pKa = 10.79 LALTKK113 pKa = 10.2 PRR115 pKa = 11.84 LAFLIVLTTTAAYY128 pKa = 10.1 SIYY131 pKa = 9.81 PVPAVLSSNVTAAPSLSTLTLAFLTTGTFLTIASANTLNMLFEE174 pKa = 4.73 PAHH177 pKa = 5.9 DD178 pKa = 3.73 AKK180 pKa = 10.52 MSRR183 pKa = 11.84 TRR185 pKa = 11.84 NRR187 pKa = 11.84 PLVRR191 pKa = 11.84 GLLSKK196 pKa = 10.35 RR197 pKa = 11.84 AAMVFAIATGVVGTGTLWLGVNPTTALLGAGNLILYY233 pKa = 9.24 AFIYY237 pKa = 10.25 TPFKK241 pKa = 10.48 RR242 pKa = 11.84 LHH244 pKa = 6.38 PVNTWVGAIVGAIPPLMGWCAAGSQYY270 pKa = 8.43 STKK273 pKa = 10.37 AASPFDD279 pKa = 3.42 IGTVWEE285 pKa = 4.11 EE286 pKa = 4.66 AKK288 pKa = 10.85 DD289 pKa = 3.91 LLCTEE294 pKa = 4.17 QAIGGWLLAALLFAWQFPHH313 pKa = 7.14 FFALSYY319 pKa = 10.83 AVRR322 pKa = 11.84 QEE324 pKa = 4.04 YY325 pKa = 10.74 AGAGYY330 pKa = 11.34 KK331 pKa = 9.3 MLTSTNVPMAARR343 pKa = 11.84 VSFRR347 pKa = 11.84 YY348 pKa = 9.48 SLLMFPICFGLIVLQGDD365 pKa = 4.0 RR366 pKa = 11.84 ACFRR370 pKa = 11.84 RR371 pKa = 11.84 HH372 pKa = 5.16 KK373 pKa = 10.25 QCHH376 pKa = 3.82 QCMDD380 pKa = 3.99 AEE382 pKa = 4.08 RR383 pKa = 11.84 GMAILASQWRR393 pKa = 11.84 EE394 pKa = 3.84 RR395 pKa = 11.84 KK396 pKa = 9.63 CC397 pKa = 3.53
Molecular weight: 43.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.677
IPC_protein 10.467
Toseland 10.409
ProMoST 10.175
Dawson 10.584
Bjellqvist 10.335
Wikipedia 10.804
Rodwell 10.76
Grimsley 10.657
Solomon 10.657
Lehninger 10.613
Nozaki 10.452
DTASelect 10.306
Thurlkill 10.452
EMBOSS 10.818
Sillero 10.511
Patrickios 10.379
IPC_peptide 10.657
IPC2_peptide 9.575
IPC2.peptide.svr19 8.442
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8072
0
8072
4031696
66
5569
499.5
55.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.286 ± 0.023
1.127 ± 0.011
5.969 ± 0.021
5.995 ± 0.026
3.46 ± 0.016
6.459 ± 0.025
2.469 ± 0.012
4.86 ± 0.018
4.83 ± 0.023
8.889 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.01
3.545 ± 0.014
5.611 ± 0.026
4.467 ± 0.021
6.126 ± 0.03
8.163 ± 0.034
6.367 ± 0.025
6.097 ± 0.021
1.372 ± 0.01
2.759 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here