Vibrio virus 2019VC1
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M5CAX0|A0A6M5CAX0_9CAUD Uncharacterized protein OS=Vibrio virus 2019VC1 OX=2761539 GN=2019VC1_48 PE=4 SV=1
MM1 pKa = 8.02 NMICKK6 pKa = 10.17 ASCSDD11 pKa = 2.88 WFTAGRR17 pKa = 11.84 VYY19 pKa = 9.86 PAAMEE24 pKa = 4.56 NPTFMISAITGDD36 pKa = 4.45 DD37 pKa = 3.49 MVSDD41 pKa = 4.02 LTVTDD46 pKa = 3.77 LTGLFQLTDD55 pKa = 3.23 IKK57 pKa = 11.01 RR58 pKa = 11.84 CLSLFAALHH67 pKa = 5.94 LSFPAYY73 pKa = 10.41 SGG75 pKa = 3.34
Molecular weight: 8.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.186
IPC2_protein 4.596
IPC_protein 4.355
Toseland 4.151
ProMoST 4.558
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.368
Rodwell 4.19
Grimsley 4.062
Solomon 4.355
Lehninger 4.317
Nozaki 4.507
DTASelect 4.8
Thurlkill 4.24
EMBOSS 4.368
Sillero 4.495
Patrickios 2.041
IPC_peptide 4.355
IPC2_peptide 4.469
IPC2.peptide.svr19 4.413
Protein with the highest isoelectric point:
>tr|A0A6M5CA15|A0A6M5CA15_9CAUD Uncharacterized protein OS=Vibrio virus 2019VC1 OX=2761539 GN=2019VC1_49 PE=4 SV=1
MM1 pKa = 7.52 RR2 pKa = 11.84 RR3 pKa = 11.84 CIFHH7 pKa = 6.93 SPLIPVRR14 pKa = 11.84 FKK16 pKa = 11.23 GLEE19 pKa = 3.55 IADD22 pKa = 4.53 NIRR25 pKa = 11.84 TAAASGRR32 pKa = 11.84 LFLRR36 pKa = 11.84 KK37 pKa = 9.4 SSQNIAIRR45 pKa = 11.84 MFNDD49 pKa = 2.96 KK50 pKa = 10.52 FINDD54 pKa = 3.81 GARR57 pKa = 11.84 NDD59 pKa = 3.79 DD60 pKa = 3.82 EE61 pKa = 5.99 RR62 pKa = 11.84 NVLAA66 pKa = 5.68
Molecular weight: 7.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.428
IPC_protein 10.277
Toseland 10.95
ProMoST 10.906
Dawson 10.965
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.023
Grimsley 10.994
Solomon 11.242
Lehninger 11.199
Nozaki 10.935
DTASelect 10.745
Thurlkill 10.921
EMBOSS 11.374
Sillero 10.921
Patrickios 10.847
IPC_peptide 11.257
IPC2_peptide 9.999
IPC2.peptide.svr19 9.029
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
13219
37
1317
191.6
21.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.041 ± 0.459
1.324 ± 0.142
5.167 ± 0.285
6.453 ± 0.287
3.873 ± 0.217
6.65 ± 0.337
2.133 ± 0.243
6.702 ± 0.293
7.3 ± 0.46
7.958 ± 0.472
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.981 ± 0.175
4.531 ± 0.235
3.465 ± 0.284
3.851 ± 0.336
5.999 ± 0.297
6.43 ± 0.249
5.878 ± 0.346
6.74 ± 0.325
1.324 ± 0.125
3.2 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here