Staphylococcus virus 92
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZAC5|Q4ZAC5_9CAUD ORF034 OS=Staphylococcus virus 92 OX=320849 PE=4 SV=1
MM1 pKa = 6.89 QQQAYY6 pKa = 9.38 INATIDD12 pKa = 2.95 IRR14 pKa = 11.84 IPTEE18 pKa = 3.45 VEE20 pKa = 3.82 YY21 pKa = 10.92 QHH23 pKa = 6.88 FDD25 pKa = 3.52 DD26 pKa = 4.52 VDD28 pKa = 3.67 KK29 pKa = 11.4 EE30 pKa = 4.28 KK31 pKa = 10.38 EE32 pKa = 4.13 TLADD36 pKa = 3.57 YY37 pKa = 10.81 LYY39 pKa = 10.9 NNPDD43 pKa = 3.71 EE44 pKa = 4.28 ILEE47 pKa = 3.92 YY48 pKa = 11.25 DD49 pKa = 3.43 NLKK52 pKa = 9.69 IRR54 pKa = 11.84 NVNVEE59 pKa = 3.93 VEE61 pKa = 4.1
Molecular weight: 7.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.068
IPC2_protein 4.075
IPC_protein 3.973
Toseland 3.795
ProMoST 4.062
Dawson 3.948
Bjellqvist 4.177
Wikipedia 3.859
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.884
Nozaki 4.075
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.088
Patrickios 3.032
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 4.019
Protein with the highest isoelectric point:
>tr|Q4ZAF2|Q4ZAF2_9CAUD ORF089 OS=Staphylococcus virus 92 OX=320849 PE=4 SV=1
MM1 pKa = 7.07 VKK3 pKa = 10.27 YY4 pKa = 10.25 RR5 pKa = 11.84 LPLSLMSQALQVIQLNQLRR24 pKa = 11.84 VHH26 pKa = 6.36 GNVINMVLTTWKK38 pKa = 10.63 KK39 pKa = 9.03 MLDD42 pKa = 3.66 SQTVINQSLL51 pKa = 3.33
Molecular weight: 5.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.248
IPC2_protein 9.838
IPC_protein 10.248
Toseland 10.73
ProMoST 10.277
Dawson 10.818
Bjellqvist 10.452
Wikipedia 10.965
Rodwell 11.345
Grimsley 10.862
Solomon 10.891
Lehninger 10.877
Nozaki 10.687
DTASelect 10.452
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.73
Patrickios 11.199
IPC_peptide 10.906
IPC2_peptide 9.033
IPC2.peptide.svr19 8.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
74
0
74
14240
36
989
192.4
22.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.779 ± 0.433
0.66 ± 0.107
6.355 ± 0.28
7.163 ± 0.386
4.27 ± 0.181
5.695 ± 0.356
1.678 ± 0.131
7.395 ± 0.212
9.199 ± 0.395
7.669 ± 0.28
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.584 ± 0.17
6.419 ± 0.207
2.992 ± 0.239
4.087 ± 0.18
4.073 ± 0.264
5.948 ± 0.284
6.243 ± 0.278
6.243 ± 0.29
1.208 ± 0.154
4.34 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here