Loktanella sp. 3ANDIMAR09
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3527 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0Q2YX32|A0A0Q2YX32_9RHOB Uncharacterized protein OS=Loktanella sp. 3ANDIMAR09 OX=1225657 GN=AN189_14305 PE=4 SV=1
MM1 pKa = 7.62 EE2 pKa = 5.32 KK3 pKa = 10.57 FRR5 pKa = 11.84 WTLSARR11 pKa = 11.84 ASRR14 pKa = 11.84 AAAFLAFLLQICVATVAGATPFTMTVPGTGVQLPAEE50 pKa = 4.34 YY51 pKa = 9.08 PQAGGVAIVMTGVNGNIYY69 pKa = 10.12 YY70 pKa = 10.25 QFSNPDD76 pKa = 3.04 GAFVGFQNTGTPTRR90 pKa = 11.84 FRR92 pKa = 11.84 GNPFTINDD100 pKa = 5.89 PIPLDD105 pKa = 3.93 CGFRR109 pKa = 11.84 SCTDD113 pKa = 3.26 YY114 pKa = 11.34 FGGAIARR121 pKa = 11.84 VDD123 pKa = 3.32 IRR125 pKa = 11.84 FSALDD130 pKa = 3.47 GDD132 pKa = 4.42 TQVGGFDD139 pKa = 3.81 RR140 pKa = 11.84 NDD142 pKa = 2.76 IFLILNGVNVGNWSDD157 pKa = 3.53 VTTQATNEE165 pKa = 3.99 AGTTSFSTQQGFGNNTFDD183 pKa = 3.75 TGWFSSTNAALLGNILQANQTVTQVFDD210 pKa = 4.37 RR211 pKa = 11.84 DD212 pKa = 3.84 PNDD215 pKa = 3.49 NFWDD219 pKa = 4.62 FTRR222 pKa = 11.84 GNSLPQDD229 pKa = 4.94 DD230 pKa = 5.0 IRR232 pKa = 11.84 TIAPGYY238 pKa = 8.98 EE239 pKa = 3.84 FEE241 pKa = 5.79 KK242 pKa = 10.61 IIDD245 pKa = 4.1 SADD248 pKa = 3.1 LTYY251 pKa = 11.15 AQVGDD256 pKa = 4.34 IIDD259 pKa = 3.34 YY260 pKa = 10.62 SYY262 pKa = 11.51 VVRR265 pKa = 11.84 NIGSVDD271 pKa = 3.0 IDD273 pKa = 5.08 DD274 pKa = 4.06 ITVEE278 pKa = 4.64 DD279 pKa = 4.06 DD280 pKa = 4.15 RR281 pKa = 11.84 IPNVVCPAPPQNSLRR296 pKa = 11.84 RR297 pKa = 11.84 TRR299 pKa = 11.84 GGDD302 pKa = 3.11 PTANEE307 pKa = 4.18 LTCTGTYY314 pKa = 10.18 RR315 pKa = 11.84 ITQADD320 pKa = 3.3 IDD322 pKa = 4.18 AGQVTNIASANGTPEE337 pKa = 3.82 FGEE340 pKa = 4.3 LGEE343 pKa = 4.41 VTDD346 pKa = 4.15 TATTTGPALVNTITLDD362 pKa = 3.36 KK363 pKa = 11.05 VGAPDD368 pKa = 3.52 PFGPVGSQVTYY379 pKa = 10.45 TLTATNTGTSTLRR392 pKa = 11.84 DD393 pKa = 3.68 VLITDD398 pKa = 4.08 PRR400 pKa = 11.84 TPGQTCTFPTLLPLSADD417 pKa = 3.31 NATNTASCTVTYY429 pKa = 9.69 TVTQADD435 pKa = 3.29 VDD437 pKa = 3.99 AFALNGTTLDD447 pKa = 3.25 NTATVRR453 pKa = 11.84 ATPPNGPAITATASDD468 pKa = 4.55 ALPGQPAVVSLTLVKK483 pKa = 10.46 SALQSTYY490 pKa = 11.17 DD491 pKa = 3.41 AVGDD495 pKa = 3.91 VIDD498 pKa = 4.48 YY499 pKa = 10.73 RR500 pKa = 11.84 FTINNTGTVTWPGPPTLTDD519 pKa = 3.49 TLTGGASCPAGPVAPGGTVVCEE541 pKa = 3.81 ASYY544 pKa = 10.31 TILQADD550 pKa = 4.48 LDD552 pKa = 4.17 AGVRR556 pKa = 11.84 PNEE559 pKa = 3.63 ATAAITVAGQTAQATDD575 pKa = 4.05 DD576 pKa = 3.83 VTVTAVVTTGLTLSKK591 pKa = 10.33 ALQAGSPNTFDD602 pKa = 3.44 APGVVLTYY610 pKa = 11.25 NFVLTNTGTVTLTNPVVDD628 pKa = 5.05 DD629 pKa = 3.73 ATTAPTVTPVPVTCPAGPIAPGGTLTCSADD659 pKa = 3.69 YY660 pKa = 11.1 AVTQADD666 pKa = 4.01 LDD668 pKa = 4.09 AGAVSNTATAAATAPPGAGGGQVLSGPQSLTVTAEE703 pKa = 3.53 RR704 pKa = 11.84 RR705 pKa = 11.84 PALNIVKK712 pKa = 8.15 TAPTLTALQFSALPVVTYY730 pKa = 9.53 TYY732 pKa = 11.36 DD733 pKa = 3.26 VTNSGNVTIPGPITVTDD750 pKa = 4.6 DD751 pKa = 3.43 KK752 pKa = 11.39 LSPQTFTCTGAAIAVGDD769 pKa = 4.35 SVQCTATYY777 pKa = 10.35 QVTPTDD783 pKa = 3.43 VARR786 pKa = 11.84 QSVRR790 pKa = 11.84 NTAFATGGDD799 pKa = 3.77 GTVSNTDD806 pKa = 3.5 DD807 pKa = 4.63 AVIAPAGRR815 pKa = 11.84 LDD817 pKa = 3.5 IQLEE821 pKa = 4.29 KK822 pKa = 10.79 QAVTTDD828 pKa = 4.01 FAAVGDD834 pKa = 4.22 PVDD837 pKa = 4.0 FTLTFTNTGDD847 pKa = 3.36 FTLFPEE853 pKa = 4.67 TDD855 pKa = 2.97 SSALDD860 pKa = 3.51 RR861 pKa = 11.84 YY862 pKa = 10.18 EE863 pKa = 3.86 LTDD866 pKa = 4.05 PGVSGLDD873 pKa = 3.16 SCIIPNPLFPQGGAQTPTQFTCTVTRR899 pKa = 11.84 IITQADD905 pKa = 3.34 VDD907 pKa = 3.95 AGSYY911 pKa = 10.12 TNTAFLTMIYY921 pKa = 10.42 NQGQVNEE928 pKa = 4.33 EE929 pKa = 4.32 TQVVPPASDD938 pKa = 3.54 TVPLNANVVPSFTLTKK954 pKa = 9.97 TATNDD959 pKa = 3.13 YY960 pKa = 10.06 GAVGDD965 pKa = 4.06 TINYY969 pKa = 9.43 DD970 pKa = 3.73 FEE972 pKa = 4.53 VTNTSVLTLTNVVLTDD988 pKa = 3.76 PKK990 pKa = 10.24 IGSISCVGSATANEE1004 pKa = 4.5 TGTIAPGASALCTGSYY1020 pKa = 10.42 EE1021 pKa = 4.17 VTQADD1026 pKa = 3.85 LDD1028 pKa = 4.0 AEE1030 pKa = 4.38 QVVNTVSALAQTQAAGSVTQTAQEE1054 pKa = 3.93 ITPIDD1059 pKa = 3.56 PAAATRR1065 pKa = 11.84 ALSLTKK1071 pKa = 9.94 IVTVRR1076 pKa = 11.84 EE1077 pKa = 4.05 TGDD1080 pKa = 3.25 ATFTAPGQTADD1091 pKa = 3.42 YY1092 pKa = 10.46 SFVVEE1097 pKa = 4.05 NTGNLTLDD1105 pKa = 3.98 NIAVVDD1111 pKa = 3.88 ADD1113 pKa = 5.17 LGYY1116 pKa = 8.65 TCQIPTLAPGATDD1129 pKa = 3.28 TATCSASRR1137 pKa = 11.84 VLTQADD1143 pKa = 4.05 FDD1145 pKa = 4.19 AGSYY1149 pKa = 10.38 QNDD1152 pKa = 3.21 AVARR1156 pKa = 11.84 AAGADD1161 pKa = 3.9 DD1162 pKa = 4.66 APASATATAQGRR1174 pKa = 11.84 IASFDD1179 pKa = 3.84 FVKK1182 pKa = 10.61 SAPASFAVAGEE1193 pKa = 4.11 VVDD1196 pKa = 5.73 FVFAFTNTGTVTLTDD1211 pKa = 3.33 ITIVDD1216 pKa = 4.02 PLLGVAYY1223 pKa = 9.72 ACEE1226 pKa = 4.14 ITSIAPGVTDD1236 pKa = 3.68 NSCFGSYY1243 pKa = 8.4 EE1244 pKa = 4.02 VQPADD1249 pKa = 3.25 VDD1251 pKa = 3.56 AGQIQNDD1258 pKa = 3.37 ASFTGVGLDD1267 pKa = 3.62 GAPISGDD1274 pKa = 3.1 DD1275 pKa = 3.71 TVIVTGPAEE1284 pKa = 4.29 APALTVTKK1292 pKa = 10.74 VEE1294 pKa = 4.53 DD1295 pKa = 4.42 DD1296 pKa = 3.67 GTGTFDD1302 pKa = 4.09 NLPATEE1308 pKa = 4.52 SYY1310 pKa = 11.29 SFVVTNTGNVTLTDD1324 pKa = 4.33 LSLVDD1329 pKa = 4.6 DD1330 pKa = 4.37 LTGFSCALPDD1340 pKa = 4.23 LAAGAATSLCADD1352 pKa = 4.69 GAPLTDD1358 pKa = 4.1 SYY1360 pKa = 11.76 TVLQTDD1366 pKa = 3.33 IDD1368 pKa = 4.06 AGALEE1373 pKa = 4.29 NTATATGRR1381 pKa = 11.84 TTRR1384 pKa = 11.84 GAGVPVEE1391 pKa = 4.64 ASDD1394 pKa = 3.75 TVTLQGPAQLPVISMVKK1411 pKa = 8.9 TATAGAGFSALGDD1424 pKa = 3.88 TVSYY1428 pKa = 10.78 DD1429 pKa = 3.49 YY1430 pKa = 11.34 TVTNDD1435 pKa = 3.27 GNITLTAPITVTDD1448 pKa = 3.63 DD1449 pKa = 3.36 RR1450 pKa = 11.84 VTVSCPALPAAGLAPGADD1468 pKa = 3.51 IVCTATDD1475 pKa = 3.61 TVDD1478 pKa = 4.66 QADD1481 pKa = 4.04 LDD1483 pKa = 3.85 AGEE1486 pKa = 4.61 IVNTATANVRR1496 pKa = 11.84 QPVSPSATYY1505 pKa = 9.4 PGGVAEE1511 pKa = 4.32 VSSNTEE1517 pKa = 4.15 TVTVTADD1524 pKa = 3.37 QQPALAITKK1533 pKa = 9.63 RR1534 pKa = 11.84 VKK1536 pKa = 10.48 PGTATSFDD1544 pKa = 3.83 APGDD1548 pKa = 3.64 LSDD1551 pKa = 5.02 PGNPNNLVYY1560 pKa = 10.65 EE1561 pKa = 4.85 FIVEE1565 pKa = 3.98 NTGNVTTTDD1574 pKa = 5.12 PITVTDD1580 pKa = 4.45 PLISATPLVCTTDD1593 pKa = 3.58 PLAPGGTVTCEE1604 pKa = 3.57 LPYY1607 pKa = 10.99 APTQEE1612 pKa = 4.23 DD1613 pKa = 3.26 VDD1615 pKa = 3.97 RR1616 pKa = 11.84 GFFANSADD1624 pKa = 3.17 AATRR1628 pKa = 11.84 FNGAPVQTPAPGTEE1642 pKa = 4.08 TVNAVQRR1649 pKa = 11.84 PALTIVKK1656 pKa = 8.64 TLEE1659 pKa = 4.05 DD1660 pKa = 3.77 LAIFSPGEE1668 pKa = 3.38 IATYY1672 pKa = 10.34 RR1673 pKa = 11.84 FYY1675 pKa = 10.89 VTNTGNTTITGPVTVTDD1692 pKa = 3.95 NKK1694 pKa = 10.1 IAGPIDD1700 pKa = 4.37 CGPGDD1705 pKa = 4.53 LAPGDD1710 pKa = 4.05 STACLATYY1718 pKa = 8.42 TVTVNDD1724 pKa = 3.58 VNAGSVTNSASASNGVTTSDD1744 pKa = 3.95 PQSLTIPVGAEE1755 pKa = 3.6 PTLSIVKK1762 pKa = 7.61 TAPGGVFAQAGDD1774 pKa = 3.71 VIAYY1778 pKa = 7.63 EE1779 pKa = 4.19 FLVTNTSSGPIPPAFVQPVTVIDD1802 pKa = 4.5 DD1803 pKa = 4.56 KK1804 pKa = 10.78 ITTPIVCPVVDD1815 pKa = 4.53 GANPLDD1821 pKa = 4.3 PGEE1824 pKa = 5.37 SITCTGSYY1832 pKa = 8.94 TVQQADD1838 pKa = 3.71 LDD1840 pKa = 4.0 AVRR1843 pKa = 11.84 PGFTRR1848 pKa = 11.84 GFVINNAFARR1858 pKa = 11.84 SEE1860 pKa = 4.04 FAGEE1864 pKa = 4.09 LVEE1867 pKa = 5.17 SPVDD1871 pKa = 3.64 QVIITADD1878 pKa = 3.75 SVPLLGVVKK1887 pKa = 9.3 TAEE1890 pKa = 4.09 NQTNPGQDD1898 pKa = 2.86 AAAGDD1903 pKa = 4.08 VVAFTIVSQNDD1914 pKa = 4.02 GPQTLTDD1921 pKa = 3.57 VRR1923 pKa = 11.84 VTDD1926 pKa = 4.13 PFLGALSCDD1935 pKa = 2.94 IASPVTLAPGAALTCTGSYY1954 pKa = 9.35 TVTQADD1960 pKa = 3.47 VDD1962 pKa = 3.87 AGGPISNTADD1972 pKa = 3.15 AAATTTQGEE1981 pKa = 4.57 AVSGTDD1987 pKa = 2.95 TTTYY1991 pKa = 10.21 PVAAPLPAMQVAKK2004 pKa = 10.5 ALEE2007 pKa = 4.6 PGSGQNDD2014 pKa = 4.23 YY2015 pKa = 9.48 NTVGEE2020 pKa = 4.4 DD2021 pKa = 3.0 LTFRR2025 pKa = 11.84 VTVTNTGNITLNDD2038 pKa = 3.49 VAVTDD2043 pKa = 4.26 DD2044 pKa = 4.99 LVPGTCTVPTLAPGASDD2061 pKa = 3.66 TSCVFVYY2068 pKa = 9.15 TVQQADD2074 pKa = 3.43 IDD2076 pKa = 4.05 RR2077 pKa = 11.84 GTITNIATAQAQPANPGAPEE2097 pKa = 4.85 LIEE2100 pKa = 4.45 TGQSDD2105 pKa = 3.56 ITGPAAEE2112 pKa = 4.87 PGVGLSKK2119 pKa = 9.84 TGSGEE2124 pKa = 3.92 VTASDD2129 pKa = 4.24 GSPTFNLPNQSLTYY2143 pKa = 9.93 VYY2145 pKa = 10.68 EE2146 pKa = 4.11 VVNLGNVTLNQLPAITDD2163 pKa = 3.47 DD2164 pKa = 4.96 RR2165 pKa = 11.84 IDD2167 pKa = 4.58 SVTCDD2172 pKa = 3.94 PLPAAGLLPGEE2183 pKa = 4.32 VLICRR2188 pKa = 11.84 GSDD2191 pKa = 2.95 TVDD2194 pKa = 3.31 QNDD2197 pKa = 2.94 VDD2199 pKa = 4.46 AGFVTNIADD2208 pKa = 3.46 VTVANDD2214 pKa = 3.35 YY2215 pKa = 11.34 GGAPLTAQAQEE2226 pKa = 4.5 TITATVAPDD2235 pKa = 3.41 LAITKK2240 pKa = 8.44 TPSITQNATAGTEE2253 pKa = 3.79 VTYY2256 pKa = 10.26 SYY2258 pKa = 10.97 RR2259 pKa = 11.84 VQNPGNVTLTDD2270 pKa = 3.05 ITLTDD2275 pKa = 4.01 FHH2277 pKa = 6.72 TSAAGVVDD2285 pKa = 5.42 LPISDD2290 pKa = 4.21 GGVIATLAPGEE2301 pKa = 4.35 EE2302 pKa = 4.26 ATLTATYY2309 pKa = 9.51 TVTQDD2314 pKa = 5.55 DD2315 pKa = 4.23 IDD2317 pKa = 3.83 SGNAVRR2323 pKa = 11.84 NTVRR2327 pKa = 11.84 VTSTLPGGDD2336 pKa = 4.07 AGPAPLTRR2344 pKa = 11.84 ARR2346 pKa = 11.84 VDD2348 pKa = 4.2 LDD2350 pKa = 3.38 PQRR2353 pKa = 11.84 PAMEE2357 pKa = 5.12 AIKK2360 pKa = 9.0 TIPSSPVPPVPGSQITYY2377 pKa = 10.47 QITLTNTGNVTLGDD2391 pKa = 3.9 PVLTDD2396 pKa = 3.08 TVRR2399 pKa = 11.84 RR2400 pKa = 11.84 INTDD2404 pKa = 2.94 ALAGEE2409 pKa = 4.37 PAVALTGGDD2418 pKa = 3.2 TGTPGLFEE2426 pKa = 4.23 VGEE2429 pKa = 4.16 VRR2431 pKa = 11.84 TYY2433 pKa = 10.99 AVSYY2437 pKa = 9.64 TLTQDD2442 pKa = 5.01 DD2443 pKa = 3.79 IDD2445 pKa = 5.23 AGGVANSVTATADD2458 pKa = 3.52 DD2459 pKa = 4.26 PLGNPVTEE2467 pKa = 4.32 VSDD2470 pKa = 3.63 NGAGDD2475 pKa = 4.03 GDD2477 pKa = 4.56 DD2478 pKa = 3.43 PTLFVIAPTPALEE2491 pKa = 4.23 VIKK2494 pKa = 10.81 VISQPASAVGEE2505 pKa = 4.2 TVVFEE2510 pKa = 3.96 ITATNTGNVTLLDD2523 pKa = 3.83 AALTDD2528 pKa = 3.37 SFTRR2532 pKa = 11.84 LDD2534 pKa = 3.72 GTPIPATPP2542 pKa = 3.65
Molecular weight: 259.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.579
IPC_protein 3.656
Toseland 3.414
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.808
Wikipedia 3.617
Rodwell 3.478
Grimsley 3.312
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.075
Thurlkill 3.465
EMBOSS 3.63
Sillero 3.783
Patrickios 1.418
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A0Q3ESF3|A0A0Q3ESF3_9RHOB Histidinol-phosphate aminotransferase OS=Loktanella sp. 3ANDIMAR09 OX=1225657 GN=AN189_14785 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.35 AGRR28 pKa = 11.84 NIINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.31 GRR39 pKa = 11.84 SKK41 pKa = 11.12 LSAA44 pKa = 3.74
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3527
0
3527
1058224
32
2634
300.0
32.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.687 ± 0.064
0.881 ± 0.012
6.887 ± 0.047
4.895 ± 0.041
3.639 ± 0.028
8.679 ± 0.052
1.989 ± 0.024
5.262 ± 0.031
2.798 ± 0.035
9.722 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.845 ± 0.024
2.636 ± 0.025
5.057 ± 0.031
3.434 ± 0.024
6.688 ± 0.044
4.87 ± 0.029
6.062 ± 0.043
7.421 ± 0.037
1.366 ± 0.02
2.182 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here