candidate division MSBL1 archaeon SCGC-AAA259I09

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1400 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133UL72|A0A133UL72_9EURY Aspartate carbamoyltransferase regulatory chain OS=candidate division MSBL1 archaeon SCGC-AAA259I09 OX=1698267 GN=pyrI PE=3 SV=1
MM1 pKa = 7.94LEE3 pKa = 3.86FNIGFRR9 pKa = 11.84AIQQRR14 pKa = 11.84HH15 pKa = 5.64LEE17 pKa = 4.22CKK19 pKa = 10.26LYY21 pKa = 10.77GISAVGMDD29 pKa = 4.72LYY31 pKa = 11.25GIFPTEE37 pKa = 3.82GRR39 pKa = 11.84LEE41 pKa = 3.91PLYY44 pKa = 10.87FDD46 pKa = 6.19DD47 pKa = 5.72SDD49 pKa = 3.85TYY51 pKa = 11.72KK52 pKa = 11.07NGLAYY57 pKa = 10.31DD58 pKa = 4.77PIFDD62 pKa = 3.86RR63 pKa = 11.84LYY65 pKa = 8.55YY66 pKa = 10.61AQGQGSPDD74 pKa = 3.0LMYY77 pKa = 11.07YY78 pKa = 10.75DD79 pKa = 5.23FATDD83 pKa = 4.42SIVDD87 pKa = 3.85TNVDD91 pKa = 3.64LSGTTYY97 pKa = 11.31AATCKK102 pKa = 10.67GGDD105 pKa = 3.53YY106 pKa = 11.12YY107 pKa = 11.14FILDD111 pKa = 4.49GDD113 pKa = 4.76DD114 pKa = 3.84ALQMVPFDD122 pKa = 3.91MDD124 pKa = 3.42TGFGGVEE131 pKa = 3.78VLVSNITGDD140 pKa = 3.62DD141 pKa = 3.15RR142 pKa = 11.84TFAMGDD148 pKa = 2.99MDD150 pKa = 4.27FTQDD154 pKa = 3.2GKK156 pKa = 10.98ILVGASRR163 pKa = 11.84DD164 pKa = 3.41GDD166 pKa = 3.62GSVLFNYY173 pKa = 10.7DD174 pKa = 2.97MDD176 pKa = 6.03ADD178 pKa = 4.14TYY180 pKa = 10.68EE181 pKa = 4.1EE182 pKa = 4.58SIVTLGTEE190 pKa = 3.98IWWLQVSFCHH200 pKa = 6.95NGTLWAVRR208 pKa = 11.84HH209 pKa = 5.91DD210 pKa = 3.6TADD213 pKa = 3.03WYY215 pKa = 10.03TVTNPLDD222 pKa = 3.71PTNAALVSAGTLGVGGEE239 pKa = 4.33GFMDD243 pKa = 4.02VAAVHH248 pKa = 6.67PCTNRR253 pKa = 3.02

Molecular weight: 27.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133UJE7|A0A133UJE7_9EURY Uncharacterized protein (Fragment) OS=candidate division MSBL1 archaeon SCGC-AAA259I09 OX=1698267 GN=AKJ37_07705 PE=4 SV=1
MM1 pKa = 7.04QVRR4 pKa = 11.84VDD6 pKa = 4.94LQTCPCQVLLQFLPRR21 pKa = 11.84SPLYY25 pKa = 10.17RR26 pKa = 11.84QILRR30 pKa = 11.84FSRR33 pKa = 11.84LHH35 pKa = 5.38YY36 pKa = 10.23KK37 pKa = 10.31VIRR40 pKa = 11.84KK41 pKa = 9.36VGEE44 pKa = 3.56EE45 pKa = 4.15AEE47 pKa = 4.14WAKK50 pKa = 10.93VEE52 pKa = 4.03EE53 pKa = 4.36RR54 pKa = 11.84SNNSRR59 pKa = 11.84ILVTRR64 pKa = 11.84EE65 pKa = 3.04WGEE68 pKa = 3.75LKK70 pKa = 10.51KK71 pKa = 10.68ALKK74 pKa = 10.35NSSKK78 pKa = 9.93QVHH81 pKa = 5.64PVRR84 pKa = 11.84VGRR87 pKa = 4.12

Molecular weight: 10.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1400

0

1400

305164

43

1838

218.0

24.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.05 ± 0.075

1.043 ± 0.03

5.746 ± 0.06

10.393 ± 0.1

3.935 ± 0.058

7.541 ± 0.075

1.633 ± 0.026

6.927 ± 0.076

7.788 ± 0.092

8.897 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.215 ± 0.031

3.692 ± 0.046

4.206 ± 0.04

2.257 ± 0.035

5.852 ± 0.066

6.179 ± 0.047

4.693 ± 0.045

6.934 ± 0.062

1.109 ± 0.028

2.908 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski