Sugarcane streak virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0Z3X5|B0Z3X5_9GEMI Replication-associated protein OS=Sugarcane streak virus OX=10836 PE=3 SV=1
MM1 pKa = 6.86STVGSSMSSAPVRR14 pKa = 11.84RR15 pKa = 11.84FKK17 pKa = 10.85HH18 pKa = 6.17RR19 pKa = 11.84NVNTFLTYY27 pKa = 9.73PRR29 pKa = 11.84CNLDD33 pKa = 3.31PEE35 pKa = 4.45AVGLIIWNLISHH47 pKa = 7.24WNPAYY52 pKa = 9.62ILSVRR57 pKa = 11.84EE58 pKa = 3.76THH60 pKa = 6.81EE61 pKa = 4.49DD62 pKa = 3.07GGYY65 pKa = 9.93HH66 pKa = 5.24IHH68 pKa = 6.93VLAQSVKK75 pKa = 9.9PVYY78 pKa = 8.44TTDD81 pKa = 3.43PAFFDD86 pKa = 4.52IDD88 pKa = 3.48NHH90 pKa = 6.32HH91 pKa = 7.35PNIQSGKK98 pKa = 8.67SANKK102 pKa = 8.62IKK104 pKa = 10.62AYY106 pKa = 8.2ITKK109 pKa = 9.74KK110 pKa = 10.15PVSIWEE116 pKa = 4.14KK117 pKa = 9.53GTFIPRR123 pKa = 11.84KK124 pKa = 8.1TSFQGDD130 pKa = 3.68STEE133 pKa = 4.36PNSKK137 pKa = 9.98KK138 pKa = 9.84QSKK141 pKa = 10.53DD142 pKa = 3.27DD143 pKa = 3.36IVRR146 pKa = 11.84DD147 pKa = 4.03IIEE150 pKa = 4.43HH151 pKa = 5.04STNKK155 pKa = 9.66QEE157 pKa = 4.09YY158 pKa = 10.37LSMIQKK164 pKa = 10.03ALPYY168 pKa = 9.73EE169 pKa = 4.28WATKK173 pKa = 9.84LQYY176 pKa = 10.43FEE178 pKa = 4.96YY179 pKa = 10.26SANKK183 pKa = 9.73LFPDD187 pKa = 3.45TQEE190 pKa = 4.51IYY192 pKa = 10.11TSPFPRR198 pKa = 11.84TTPNLLDD205 pKa = 3.41TTTINTWLQNNLYY218 pKa = 9.7QVSPQAYY225 pKa = 8.22MLTNPSCLTLEE236 pKa = 4.35EE237 pKa = 4.43AASDD241 pKa = 4.04LTWMAEE247 pKa = 4.0TSRR250 pKa = 11.84ILIPTGSSASTSSGQQEE267 pKa = 4.05RR268 pKa = 11.84ASQHH272 pKa = 5.23GQEE275 pKa = 4.02AWEE278 pKa = 4.87DD279 pKa = 3.75IITGRR284 pKa = 11.84TTSTGSSYY292 pKa = 11.64DD293 pKa = 3.16EE294 pKa = 4.22DD295 pKa = 4.2AEE297 pKa = 4.35YY298 pKa = 11.16NIIDD302 pKa = 5.47DD303 pKa = 4.95IPFKK307 pKa = 11.07YY308 pKa = 9.94CPCWKK313 pKa = 10.13QLIGCQKK320 pKa = 10.89DD321 pKa = 3.8YY322 pKa = 11.15IVNPKK327 pKa = 8.71YY328 pKa = 10.79GKK330 pKa = 9.49RR331 pKa = 11.84KK332 pKa = 9.39KK333 pKa = 9.61VAHH336 pKa = 6.49KK337 pKa = 10.25SIPTIVLANEE347 pKa = 4.03DD348 pKa = 3.98EE349 pKa = 5.0DD350 pKa = 3.86WFKK353 pKa = 11.84DD354 pKa = 3.56MSPAQQDD361 pKa = 3.84YY362 pKa = 11.01FNANCTTYY370 pKa = 10.06MLEE373 pKa = 3.93PGEE376 pKa = 4.96RR377 pKa = 11.84FFSPPAVSATAHH389 pKa = 6.68PSDD392 pKa = 4.07EE393 pKa = 4.36VV394 pKa = 3.32

Molecular weight: 44.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0Z3X5|B0Z3X5_9GEMI Replication-associated protein OS=Sugarcane streak virus OX=10836 PE=3 SV=1
MM1 pKa = 7.68PLSGMKK7 pKa = 10.2RR8 pKa = 11.84KK9 pKa = 10.1RR10 pKa = 11.84SDD12 pKa = 2.69EE13 pKa = 3.64AARR16 pKa = 11.84RR17 pKa = 11.84KK18 pKa = 10.11RR19 pKa = 11.84MSGAKK24 pKa = 9.27QGRR27 pKa = 11.84TSAARR32 pKa = 11.84VAPSVRR38 pKa = 11.84KK39 pKa = 9.57IRR41 pKa = 11.84PSLQIQTLQAAGQTMISVPSGGVCEE66 pKa = 4.18LLGTFSRR73 pKa = 11.84GSDD76 pKa = 2.89EE77 pKa = 4.71GNRR80 pKa = 11.84HH81 pKa = 4.56TNEE84 pKa = 3.59TVIYY88 pKa = 9.51KK89 pKa = 10.32VSLDD93 pKa = 3.51YY94 pKa = 11.48HH95 pKa = 6.38FVATAASCKK104 pKa = 9.73YY105 pKa = 10.25SSIGTGVVWLVYY117 pKa = 10.04DD118 pKa = 4.33AQPSGNAPTVKK129 pKa = 10.33DD130 pKa = 3.86IFPHH134 pKa = 7.03PDD136 pKa = 2.84TLTAFPYY143 pKa = 7.28TWKK146 pKa = 10.49VGRR149 pKa = 11.84EE150 pKa = 3.83VCHH153 pKa = 6.21RR154 pKa = 11.84FVVKK158 pKa = 10.56RR159 pKa = 11.84RR160 pKa = 11.84YY161 pKa = 7.89TFTMEE166 pKa = 3.63VNGRR170 pKa = 11.84IGSDD174 pKa = 3.51VPGSTSCWPPCRR186 pKa = 11.84RR187 pKa = 11.84NIYY190 pKa = 7.82FHH192 pKa = 7.11KK193 pKa = 10.11FVTGLGVKK201 pKa = 7.98TEE203 pKa = 4.14WKK205 pKa = 8.16NTTGGDD211 pKa = 3.17VGDD214 pKa = 3.87IKK216 pKa = 10.91KK217 pKa = 10.04GALYY221 pKa = 10.22IVVAPGNGLEE231 pKa = 4.27FTVHH235 pKa = 5.73GNARR239 pKa = 11.84LYY241 pKa = 10.33FKK243 pKa = 10.94SVGNQQ248 pKa = 2.8

Molecular weight: 27.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

750

108

394

250.0

27.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.067 ± 0.65

1.6 ± 0.195

4.933 ± 0.665

4.667 ± 0.812

3.867 ± 0.214

6.8 ± 2.077

2.267 ± 0.472

5.467 ± 1.087

5.6 ± 0.935

6.0 ± 0.856

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.733 ± 0.173

4.0 ± 0.969

6.667 ± 0.69

3.733 ± 0.721

5.6 ± 1.525

9.067 ± 0.643

7.867 ± 0.678

6.933 ± 1.91

2.267 ± 0.342

3.867 ± 0.703
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski