Sewage-associated circular DNA virus-9

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; unclassified Smacoviridae

Average proteome isoelectric point is 4.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075IZU5|A0A075IZU5_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-9 OX=1519398 PE=4 SV=1
MM1 pKa = 7.17VFVKK5 pKa = 10.66VSEE8 pKa = 4.46TYY10 pKa = 10.74DD11 pKa = 3.11LSTNVGKK18 pKa = 9.83MGIVGIHH25 pKa = 5.33TPKK28 pKa = 10.77GDD30 pKa = 4.18LISRR34 pKa = 11.84LWGGLAMQHH43 pKa = 6.23KK44 pKa = 9.1YY45 pKa = 9.14VRR47 pKa = 11.84FDD49 pKa = 3.43SCDD52 pKa = 3.07VTMACASLLPADD64 pKa = 4.64PLQIGQEE71 pKa = 4.04AGSIAPQDD79 pKa = 3.62MFNPILYY86 pKa = 9.77KK87 pKa = 10.6AVSNDD92 pKa = 3.23SMSNLQAFLSATATRR107 pKa = 11.84DD108 pKa = 3.71SQIGTAKK115 pKa = 10.57GSVVDD120 pKa = 5.18LNDD123 pKa = 4.25PNFQFNDD130 pKa = 3.7SVPINQFDD138 pKa = 3.25IYY140 pKa = 11.11YY141 pKa = 10.13SLLGNPDD148 pKa = 3.42GFKK151 pKa = 10.78KK152 pKa = 10.64AMPQAGLQMRR162 pKa = 11.84GLYY165 pKa = 9.43PLVYY169 pKa = 9.87QVVSNYY175 pKa = 9.62GVNTAGMTDD184 pKa = 3.35SDD186 pKa = 5.03SIGNNSNPSNPIYY199 pKa = 10.96GPDD202 pKa = 3.57FQDD205 pKa = 3.35GVTPLDD211 pKa = 3.24ISNIQLRR218 pKa = 11.84GPAMRR223 pKa = 11.84MPRR226 pKa = 11.84VNTTYY231 pKa = 10.87LPYY234 pKa = 10.91NSIQNAPLSGKK245 pKa = 10.55ANLNFGDD252 pKa = 4.32TTRR255 pKa = 11.84GMPPCYY261 pKa = 9.6VAMIILPPATLNKK274 pKa = 10.11LYY276 pKa = 10.84YY277 pKa = 9.18RR278 pKa = 11.84LKK280 pKa = 8.98VTWTIEE286 pKa = 4.01FTEE289 pKa = 4.39LRR291 pKa = 11.84SLDD294 pKa = 4.5EE295 pKa = 4.02ISNWRR300 pKa = 11.84GMSEE304 pKa = 3.73IGVDD308 pKa = 4.48VYY310 pKa = 11.14ATDD313 pKa = 3.71YY314 pKa = 8.85ATQSAALMSDD324 pKa = 3.78MEE326 pKa = 4.83SMVDD330 pKa = 3.23VGNANIQKK338 pKa = 9.94IMEE341 pKa = 4.25GRR343 pKa = 3.39

Molecular weight: 37.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075IZU5|A0A075IZU5_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-9 OX=1519398 PE=4 SV=1
MM1 pKa = 7.59ANVKK5 pKa = 9.67WIDD8 pKa = 3.29ATIWCEE14 pKa = 3.33GDD16 pKa = 3.2NAFEE20 pKa = 4.17GVKK23 pKa = 10.6DD24 pKa = 3.7EE25 pKa = 4.5SEE27 pKa = 3.79WRR29 pKa = 11.84SRR31 pKa = 11.84FAGLFEE37 pKa = 4.63RR38 pKa = 11.84YY39 pKa = 9.68AYY41 pKa = 10.25GRR43 pKa = 11.84EE44 pKa = 3.98TAPEE48 pKa = 3.63TGRR51 pKa = 11.84RR52 pKa = 11.84HH53 pKa = 4.37FQFRR57 pKa = 11.84GVLKK61 pKa = 9.28VACDD65 pKa = 4.15ASCLAYY71 pKa = 10.54LSSLGFRR78 pKa = 11.84NISPTHH84 pKa = 5.72VRR86 pKa = 11.84DD87 pKa = 3.2FDD89 pKa = 3.93YY90 pKa = 11.16VYY92 pKa = 11.01KK93 pKa = 10.98DD94 pKa = 3.39RR95 pKa = 11.84DD96 pKa = 4.16FFCSWDD102 pKa = 3.66VYY104 pKa = 10.45RR105 pKa = 11.84PEE107 pKa = 4.17YY108 pKa = 10.9DD109 pKa = 3.23LVRR112 pKa = 11.84NSPHH116 pKa = 4.7VWQVQLEE123 pKa = 4.14DD124 pKa = 3.68MEE126 pKa = 6.7RR127 pKa = 11.84DD128 pKa = 3.32DD129 pKa = 4.0RR130 pKa = 11.84TIEE133 pKa = 4.08IVWDD137 pKa = 3.43EE138 pKa = 4.43RR139 pKa = 11.84GNSGKK144 pKa = 7.63TAWAMYY150 pKa = 10.23QDD152 pKa = 4.4YY153 pKa = 10.37LHH155 pKa = 6.9KK156 pKa = 10.36AVYY159 pKa = 9.78IPPPQRR165 pKa = 11.84GLDD168 pKa = 3.56LVACVLGKK176 pKa = 10.23RR177 pKa = 11.84EE178 pKa = 4.14SDD180 pKa = 3.16WYY182 pKa = 10.45IIDD185 pKa = 3.59TPRR188 pKa = 11.84AFEE191 pKa = 4.3FTDD194 pKa = 3.67DD195 pKa = 3.64WACSIEE201 pKa = 3.98QLKK204 pKa = 10.52NGYY207 pKa = 9.48VFDD210 pKa = 3.62TRR212 pKa = 11.84YY213 pKa = 10.29SFRR216 pKa = 11.84DD217 pKa = 3.26RR218 pKa = 11.84YY219 pKa = 10.56LSVRR223 pKa = 11.84PRR225 pKa = 11.84VTVLCNTLPEE235 pKa = 4.06YY236 pKa = 10.58EE237 pKa = 4.87RR238 pKa = 11.84YY239 pKa = 9.74FSADD243 pKa = 2.79RR244 pKa = 11.84VLPFRR249 pKa = 11.84ITPEE253 pKa = 4.44GYY255 pKa = 9.44LWSVV259 pKa = 3.16

Molecular weight: 30.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

602

259

343

301.0

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.645 ± 0.281

1.661 ± 0.627

7.807 ± 0.645

4.319 ± 1.583

4.319 ± 0.886

6.645 ± 0.978

0.997 ± 0.329

5.15 ± 0.775

3.322 ± 0.14

7.475 ± 0.548

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.488 ± 1.402

5.316 ± 1.572

5.648 ± 0.61

3.654 ± 0.805

6.312 ± 2.242

7.143 ± 0.813

5.316 ± 0.411

7.143 ± 0.348

2.159 ± 1.024

5.482 ± 0.651
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski