Streptococcus phage Javan626

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BH03|A0A4D6BH03_9CAUD Uncharacterized protein OS=Streptococcus phage Javan626 OX=2548286 GN=Javan626_0014 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 9.33ITWTGQPTNKK12 pKa = 9.54VVTYY16 pKa = 10.24KK17 pKa = 10.32IEE19 pKa = 4.33ADD21 pKa = 3.51SYY23 pKa = 11.72KK24 pKa = 10.67EE25 pKa = 3.73ILEE28 pKa = 3.99QMIDD32 pKa = 3.35RR33 pKa = 11.84DD34 pKa = 4.06LADD37 pKa = 5.24GYY39 pKa = 10.47WNPDD43 pKa = 2.64SQAFHH48 pKa = 6.68EE49 pKa = 4.35LAYY52 pKa = 9.74YY53 pKa = 10.38SPIISEE59 pKa = 5.89LEE61 pKa = 3.84DD62 pKa = 3.79EE63 pKa = 4.81YY64 pKa = 12.02NNLDD68 pKa = 4.79DD69 pKa = 6.3SDD71 pKa = 6.19DD72 pKa = 3.54EE73 pKa = 4.65DD74 pKa = 4.35AFEE77 pKa = 5.95KK78 pKa = 10.65FDD80 pKa = 3.71EE81 pKa = 4.47KK82 pKa = 11.05LGNVEE87 pKa = 3.75WNEE90 pKa = 3.87ILNSLSDD97 pKa = 3.64NEE99 pKa = 4.17MEE101 pKa = 4.8QVIRR105 pKa = 11.84GCNSQAYY112 pKa = 7.86YY113 pKa = 10.76QEE115 pKa = 4.88FEE117 pKa = 4.16VSEE120 pKa = 4.21

Molecular weight: 14.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BG65|A0A4D6BG65_9CAUD Uncharacterized protein OS=Streptococcus phage Javan626 OX=2548286 GN=Javan626_0009 PE=4 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84RR4 pKa = 11.84LLTEE8 pKa = 3.86EE9 pKa = 3.58QHH11 pKa = 6.74QFFIKK16 pKa = 10.44NVEE19 pKa = 4.19GKK21 pKa = 10.12SDD23 pKa = 3.43VRR25 pKa = 11.84MTEE28 pKa = 3.72LMNEE32 pKa = 4.0KK33 pKa = 10.42FGLSLSNKK41 pKa = 7.67QVHH44 pKa = 6.7AYY46 pKa = 9.05KK47 pKa = 10.49CNHH50 pKa = 7.21KK51 pKa = 10.05IRR53 pKa = 11.84NNVPHH58 pKa = 6.79SNRR61 pKa = 11.84NQGIRR66 pKa = 11.84LFNAEE71 pKa = 3.48QFAYY75 pKa = 10.17FKK77 pKa = 10.21TIYY80 pKa = 9.9KK81 pKa = 10.33GRR83 pKa = 11.84LSKK86 pKa = 9.81EE87 pKa = 3.88TADD90 pKa = 5.29LINKK94 pKa = 9.31KK95 pKa = 9.98FGTSFTPLQIRR106 pKa = 11.84AFRR109 pKa = 11.84RR110 pKa = 11.84NNGFDD115 pKa = 3.28SGVSGRR121 pKa = 11.84FQKK124 pKa = 10.7GVPSYY129 pKa = 11.56NKK131 pKa = 9.7GKK133 pKa = 10.39KK134 pKa = 9.8FPGRR138 pKa = 11.84TNSGTFKK145 pKa = 10.66SGHH148 pKa = 5.33TPVGQKK154 pKa = 10.03PVGSIVKK161 pKa = 9.25TKK163 pKa = 10.73DD164 pKa = 2.95GYY166 pKa = 11.29HH167 pKa = 6.86KK168 pKa = 10.62IKK170 pKa = 10.59IADD173 pKa = 3.57PKK175 pKa = 10.26TWVLCHH181 pKa = 6.28RR182 pKa = 11.84YY183 pKa = 8.49EE184 pKa = 4.18WEE186 pKa = 3.99KK187 pKa = 11.3HH188 pKa = 5.58NGPIPDD194 pKa = 4.46GYY196 pKa = 9.32TVAFLDD202 pKa = 4.19GDD204 pKa = 3.82RR205 pKa = 11.84SNWDD209 pKa = 2.94ISNLCLVSKK218 pKa = 11.37GEE220 pKa = 3.91LAGMNMKK227 pKa = 10.27RR228 pKa = 11.84LFSTDD233 pKa = 3.36PEE235 pKa = 4.32LTKK238 pKa = 10.92AGLSVLRR245 pKa = 11.84IEE247 pKa = 4.41SKK249 pKa = 10.15IRR251 pKa = 11.84EE252 pKa = 4.18VQKK255 pKa = 11.18

Molecular weight: 29.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12529

37

1349

223.7

25.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.574 ± 0.618

0.591 ± 0.122

6.465 ± 0.357

7.295 ± 0.439

3.719 ± 0.246

6.465 ± 0.333

1.285 ± 0.134

6.369 ± 0.2

8.261 ± 0.325

8.54 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.498 ± 0.177

5.715 ± 0.271

2.865 ± 0.158

3.624 ± 0.229

3.855 ± 0.264

6.665 ± 0.422

6.832 ± 0.509

6.194 ± 0.187

1.357 ± 0.138

3.831 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski