Proteus penneri ATCC 35198
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4909 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0AY89|C0AY89_9GAMM Transporter DASS family OS=Proteus penneri ATCC 35198 OX=471881 GN=PROPEN_02812 PE=4 SV=1
MM1 pKa = 8.32 DD2 pKa = 6.26 PLTPQLMDD10 pKa = 3.15 ICAIQEE16 pKa = 4.18 TYY18 pKa = 10.3 GQNKK22 pKa = 7.12 ATRR25 pKa = 11.84 IGNTIYY31 pKa = 10.89 GFNSNTKK38 pKa = 9.56 KK39 pKa = 10.56 DD40 pKa = 3.87 YY41 pKa = 11.12 YY42 pKa = 10.52 SLKK45 pKa = 9.45 TSEE48 pKa = 4.65 DD49 pKa = 3.8 KK50 pKa = 10.82 IVACIWDD57 pKa = 3.55 TGGIDD62 pKa = 3.33 TLDD65 pKa = 3.51 FSKK68 pKa = 10.3 YY69 pKa = 9.15 TVDD72 pKa = 4.73 QKK74 pKa = 11.25 IDD76 pKa = 3.71 LNEE79 pKa = 4.14 GSFSDD84 pKa = 4.56 VGGLRR89 pKa = 11.84 ANISIAYY96 pKa = 9.3 DD97 pKa = 3.47 AVIEE101 pKa = 4.0 NAIGGSQTDD110 pKa = 3.98 IISGNDD116 pKa = 3.0 ANNRR120 pKa = 11.84 LFGKK124 pKa = 10.34 LGEE127 pKa = 4.26 DD128 pKa = 3.12 TLYY131 pKa = 11.29 GKK133 pKa = 10.49 GGNDD137 pKa = 2.88 ILYY140 pKa = 10.0 GGQGNDD146 pKa = 2.96 HH147 pKa = 7.07 LYY149 pKa = 10.95 GEE151 pKa = 4.82 KK152 pKa = 11.02 GNDD155 pKa = 3.39 YY156 pKa = 10.97 LYY158 pKa = 11.15 GEE160 pKa = 4.37 QDD162 pKa = 2.98 NDD164 pKa = 3.65 VLVDD168 pKa = 3.69 SDD170 pKa = 5.23 GNDD173 pKa = 3.27 HH174 pKa = 7.24 LYY176 pKa = 10.93 GGQGDD181 pKa = 4.34 DD182 pKa = 4.18 YY183 pKa = 11.23 LWDD186 pKa = 3.85 SEE188 pKa = 4.52 GDD190 pKa = 3.69 NILDD194 pKa = 4.02 GGLGDD199 pKa = 5.06 DD200 pKa = 4.34 VLFGGDD206 pKa = 5.25 GDD208 pKa = 5.07 DD209 pKa = 4.03 EE210 pKa = 5.36 LNGGEE215 pKa = 4.76 GNDD218 pKa = 3.91 HH219 pKa = 7.47 LDD221 pKa = 3.48 PGMGNNTLKK230 pKa = 11.02 GGTGYY235 pKa = 8.59 DD236 pKa = 3.03 TFSFNTQDD244 pKa = 3.51 NDD246 pKa = 3.2 SSNIITDD253 pKa = 4.3 FEE255 pKa = 4.64 SGIDD259 pKa = 3.43 KK260 pKa = 11.02 LFFLL264 pKa = 5.49
Molecular weight: 28.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.478
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.389
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.151
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.846
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|C0ASR5|C0ASR5_9GAMM Glutamine--fructose-6-phosphate transaminase (Isomerizing) OS=Proteus penneri ATCC 35198 OX=471881 GN=PROPEN_00846 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.65 RR12 pKa = 11.84 NRR14 pKa = 11.84 NHH16 pKa = 6.8 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSSKK47 pKa = 11.16
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4909
0
4909
1040949
24
1676
212.0
23.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.978 ± 0.038
1.106 ± 0.015
5.107 ± 0.029
5.958 ± 0.038
4.165 ± 0.032
6.684 ± 0.038
2.148 ± 0.019
7.313 ± 0.035
5.689 ± 0.037
10.427 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.766 ± 0.02
4.762 ± 0.033
3.996 ± 0.024
4.442 ± 0.037
4.657 ± 0.033
6.425 ± 0.031
5.451 ± 0.025
6.393 ± 0.031
1.279 ± 0.015
3.255 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here