Schizosaccharomyces cryophilus (strain OY26 / ATCC MYA-4695 / CBS 11777 / NBRC 106824 / NRRL Y48691) (Fission yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces cryophilus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S9WZD0|S9WZD0_SCHCR Succinate dehydrogenase cytochrome b subunit OS=Schizosaccharomyces cryophilus (strain OY26 / ATCC MYA-4695 / CBS 11777 / NBRC 106824 / NRRL Y48691) OX=653667 GN=SPOG_03546 PE=3 SV=1
MM1 pKa = 7.36TEE3 pKa = 4.16SEE5 pKa = 4.54KK6 pKa = 10.72KK7 pKa = 10.42DD8 pKa = 3.4PYY10 pKa = 11.11SVIGRR15 pKa = 11.84DD16 pKa = 3.3YY17 pKa = 10.54LYY19 pKa = 10.24PVGHH23 pKa = 6.36EE24 pKa = 4.2LFLGFHH30 pKa = 6.4ARR32 pKa = 11.84VIEE35 pKa = 4.18KK36 pKa = 10.68AEE38 pKa = 3.81EE39 pKa = 3.96QYY41 pKa = 11.55EE42 pKa = 4.33DD43 pKa = 4.07NEE45 pKa = 4.46HH46 pKa = 6.74VDD48 pKa = 3.58DD49 pKa = 5.29SEE51 pKa = 5.35IINGDD56 pKa = 3.26ADD58 pKa = 4.18SIKK61 pKa = 10.88SEE63 pKa = 4.31VEE65 pKa = 4.34DD66 pKa = 4.8PDD68 pKa = 4.87DD69 pKa = 4.97SNTIQAVCDD78 pKa = 4.77AIALYY83 pKa = 10.58DD84 pKa = 4.12FEE86 pKa = 5.04PLHH89 pKa = 6.18EE90 pKa = 4.69NEE92 pKa = 4.66LGVFEE97 pKa = 5.72GQTLWILSEE106 pKa = 4.6ASHH109 pKa = 6.23GWLVAYY115 pKa = 10.23DD116 pKa = 4.03EE117 pKa = 5.18LSGKK121 pKa = 10.23SGLVPEE127 pKa = 5.06SYY129 pKa = 9.16VTLIQQ134 pKa = 3.88

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S9XCC8|S9XCC8_SCHCR E3 ubiquitin-protein ligase OS=Schizosaccharomyces cryophilus (strain OY26 / ATCC MYA-4695 / CBS 11777 / NBRC 106824 / NRRL Y48691) OX=653667 GN=SPOG_02662 PE=4 SV=1
MM1 pKa = 7.89PSNKK5 pKa = 8.19TFRR8 pKa = 11.84TKK10 pKa = 10.49QKK12 pKa = 9.65LAKK15 pKa = 9.74AQRR18 pKa = 11.84QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIHH36 pKa = 6.66YY37 pKa = 9.5NMKK40 pKa = 8.82RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.27WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 11.06LNLL51 pKa = 3.44

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5135

15

5150

2332915

44

4958

453.0

51.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.226 ± 0.03

1.408 ± 0.013

5.349 ± 0.02

6.675 ± 0.031

4.622 ± 0.022

4.972 ± 0.029

2.336 ± 0.012

5.856 ± 0.023

6.244 ± 0.026

9.872 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.012

5.08 ± 0.019

4.898 ± 0.029

4.077 ± 0.02

5.089 ± 0.023

9.323 ± 0.037

5.325 ± 0.016

5.908 ± 0.024

1.131 ± 0.011

3.456 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski