Rhodanobacter sp. B05

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Rhodanobacter; unclassified Rhodanobacter

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V3Q1Z6|A0A1V3Q1Z6_9GAMM ABC transporter permease OS=Rhodanobacter sp. B05 OX=1945859 GN=B0E47_06755 PE=3 SV=1
MM1 pKa = 7.03SQSSPEE7 pKa = 3.85ATVLRR12 pKa = 11.84KK13 pKa = 9.08WMCVVCGYY21 pKa = 10.36IYY23 pKa = 10.71DD24 pKa = 4.09EE25 pKa = 4.5AVGVPDD31 pKa = 3.87EE32 pKa = 5.73DD33 pKa = 3.8IPAGTRR39 pKa = 11.84WEE41 pKa = 4.49DD42 pKa = 3.59VPEE45 pKa = 4.08TWTCPDD51 pKa = 3.96CGTTKK56 pKa = 11.01DD57 pKa = 3.95DD58 pKa = 4.27FEE60 pKa = 5.03MIEE63 pKa = 3.83IDD65 pKa = 3.43

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V3Q843|A0A1V3Q843_9GAMM Uncharacterized protein OS=Rhodanobacter sp. B05 OX=1945859 GN=B0E47_03055 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.06LKK11 pKa = 10.38RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATADD26 pKa = 3.12GRR28 pKa = 11.84KK29 pKa = 9.4IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.92RR41 pKa = 11.84LIPP44 pKa = 4.02

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3234

0

3234

1122722

30

3095

347.2

37.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.514 ± 0.062

0.852 ± 0.013

5.805 ± 0.036

4.868 ± 0.046

3.445 ± 0.024

8.434 ± 0.04

2.599 ± 0.023

4.365 ± 0.029

2.936 ± 0.036

11.013 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.018

2.64 ± 0.031

5.338 ± 0.031

3.948 ± 0.027

7.159 ± 0.048

5.488 ± 0.032

4.943 ± 0.04

7.287 ± 0.036

1.586 ± 0.018

2.536 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski