Cowpea mild mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5KJF5|E5KJF5_9VIRU Capsid protein OS=Cowpea mild mottle virus OX=67761 PE=3 SV=1
MM1 pKa = 6.99FAAGVTAFVIILSTLILALISLGFYY26 pKa = 10.59NYY28 pKa = 10.45LSSSCVLYY36 pKa = 9.37ITGEE40 pKa = 4.04SFSISNCPVNSDD52 pKa = 3.37LANLVRR58 pKa = 11.84EE59 pKa = 4.03QRR61 pKa = 11.84EE62 pKa = 4.06LLSCGLKK69 pKa = 10.46

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5KJF6|E5KJF6_9VIRU RNA silencing suppressor OS=Cowpea mild mottle virus OX=67761 PE=3 SV=1
MM1 pKa = 7.54IGYY4 pKa = 9.35KK5 pKa = 10.12RR6 pKa = 11.84IAILLHH12 pKa = 5.76LCSLKK17 pKa = 9.95MNKK20 pKa = 8.51MLPLDD25 pKa = 4.0LCILIALKK33 pKa = 10.67AGPTSLYY40 pKa = 10.48SGSSSYY46 pKa = 11.2ARR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84AKK53 pKa = 10.68LIGRR57 pKa = 11.84CHH59 pKa = 5.78RR60 pKa = 11.84CYY62 pKa = 10.01RR63 pKa = 11.84VSPGFYY69 pKa = 9.14FTTRR73 pKa = 11.84CDD75 pKa = 3.72GLSCVPGISYY85 pKa = 8.98KK86 pKa = 10.29QWVEE90 pKa = 3.59SFIRR94 pKa = 11.84FGHH97 pKa = 5.31FTRR100 pKa = 11.84EE101 pKa = 3.85EE102 pKa = 3.92

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2654

69

1836

442.3

50.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.576 ± 0.638

2.261 ± 0.521

4.748 ± 0.481

7.046 ± 1.039

5.727 ± 0.432

5.539 ± 0.424

2.562 ± 0.31

5.426 ± 0.329

7.46 ± 0.7

11.718 ± 0.681

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.62 ± 0.241

4.559 ± 0.29

3.768 ± 0.649

2.826 ± 0.491

5.049 ± 0.448

8.478 ± 0.738

5.2 ± 0.6

6.255 ± 0.54

0.867 ± 0.192

3.316 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski