Giant panda anellovirus
Average proteome isoelectric point is 7.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGG7|A0A220IGG7_9VIRU ORF3 OS=Giant panda anellovirus OX=2016460 PE=4 SV=1
MM1 pKa = 7.39 NPNSFHH7 pKa = 6.28 GRR9 pKa = 11.84 AILQFKK15 pKa = 9.73 KK16 pKa = 10.44 KK17 pKa = 8.98 QALWFQSCSFSHH29 pKa = 7.32 KK30 pKa = 10.36 LFCQCGDD37 pKa = 3.46 WQVHH41 pKa = 4.56 LQRR44 pKa = 11.84 YY45 pKa = 8.7 RR46 pKa = 11.84 EE47 pKa = 3.99 QQWKK51 pKa = 9.83 CITGGGHH58 pKa = 6.47 TDD60 pKa = 3.21 LGDD63 pKa = 3.11 ISFVTEE69 pKa = 4.98 DD70 pKa = 4.53 GDD72 pKa = 4.21 TQGDD76 pKa = 3.7 TGGEE80 pKa = 4.01 PGGDD84 pKa = 3.45 DD85 pKa = 3.34 TGGLGG90 pKa = 4.1
Molecular weight: 9.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.473
IPC2_protein 5.461
IPC_protein 5.41
Toseland 5.766
ProMoST 5.753
Dawson 5.664
Bjellqvist 5.677
Wikipedia 5.677
Rodwell 5.626
Grimsley 5.881
Solomon 5.664
Lehninger 5.652
Nozaki 5.919
DTASelect 6.122
Thurlkill 6.097
EMBOSS 6.059
Sillero 6.008
Patrickios 3.897
IPC_peptide 5.677
IPC2_peptide 6.02
IPC2.peptide.svr19 6.079
Protein with the highest isoelectric point:
>tr|A0A220IGG9|A0A220IGG9_9VIRU Capsid protein OS=Giant panda anellovirus OX=2016460 PE=3 SV=1
MM1 pKa = 6.11 YY2 pKa = 10.0 HH3 pKa = 6.88 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 SYY8 pKa = 9.88 RR9 pKa = 11.84 PRR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 5.84 FFRR16 pKa = 11.84 HH17 pKa = 5.08 RR18 pKa = 11.84 RR19 pKa = 11.84 WRR21 pKa = 11.84 YY22 pKa = 8.2 PRR24 pKa = 11.84 RR25 pKa = 11.84 HH26 pKa = 5.29 WRR28 pKa = 11.84 RR29 pKa = 11.84 AWRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 9.25 RR36 pKa = 11.84 RR37 pKa = 11.84 PRR39 pKa = 11.84 VRR41 pKa = 11.84 AIIQQQPRR49 pKa = 11.84 RR50 pKa = 11.84 KK51 pKa = 9.62 KK52 pKa = 9.54 ILVVTGWEE60 pKa = 3.69 ILGQIGSQLKK70 pKa = 9.67 YY71 pKa = 11.01 VYY73 pKa = 10.58 DD74 pKa = 4.83 DD75 pKa = 3.44 NTKK78 pKa = 9.59 KK79 pKa = 10.45 PRR81 pKa = 11.84 VEE83 pKa = 4.54 VINIAPMNKK92 pKa = 8.26 QVKK95 pKa = 9.22 YY96 pKa = 10.04 LMGMIPQGLANTCSDD111 pKa = 2.86 TWTDD115 pKa = 2.85 GMKK118 pKa = 10.19 RR119 pKa = 11.84 RR120 pKa = 11.84 EE121 pKa = 3.98 EE122 pKa = 4.38 GGLGMTGPTHH132 pKa = 6.53 WDD134 pKa = 3.6 FVGGWGYY141 pKa = 11.67 AKK143 pKa = 10.37 FDD145 pKa = 3.93 LQSLILRR152 pKa = 11.84 NLLGMNRR159 pKa = 11.84 FSEE162 pKa = 4.45 NIRR165 pKa = 11.84 TYY167 pKa = 8.14 THH169 pKa = 6.47 IKK171 pKa = 9.26 FLKK174 pKa = 10.4 FKK176 pKa = 9.71 IQLVPGPSVDD186 pKa = 3.4 YY187 pKa = 10.8 LFRR190 pKa = 11.84 IQQHH194 pKa = 6.62 RR195 pKa = 11.84 GPQDD199 pKa = 2.93 WEE201 pKa = 4.25 EE202 pKa = 4.27 PLLHH206 pKa = 6.85 PAHH209 pKa = 7.14 LLNMPFVTWVQSIKK223 pKa = 10.34 RR224 pKa = 11.84 SKK226 pKa = 8.34 CCRR229 pKa = 11.84 MKK231 pKa = 10.47 VLRR234 pKa = 11.84 RR235 pKa = 11.84 RR236 pKa = 11.84 AGTDD240 pKa = 3.16 LNGWYY245 pKa = 10.25 DD246 pKa = 3.44 MEE248 pKa = 4.63 SFRR251 pKa = 11.84 NYY253 pKa = 10.69 EE254 pKa = 4.01 LFSYY258 pKa = 8.48 MWSAFDD264 pKa = 4.69 PNNPLGKK271 pKa = 10.19 NPILPGDD278 pKa = 3.64 TGGASTTTKK287 pKa = 8.32 WWNDD291 pKa = 2.27 DD292 pKa = 2.69 WMRR295 pKa = 11.84 ARR297 pKa = 11.84 QSPRR301 pKa = 11.84 DD302 pKa = 3.56 QKK304 pKa = 11.2 ISINMKK310 pKa = 10.26 LDD312 pKa = 2.96 WTQRR316 pKa = 11.84 NTYY319 pKa = 10.41 DD320 pKa = 3.34 NNFVKK325 pKa = 10.71 SIDD328 pKa = 3.74 PEE330 pKa = 4.01 QLTNEE335 pKa = 4.37 TTNFWDD341 pKa = 3.13 WLLGKK346 pKa = 10.0 SPPQGKK352 pKa = 9.54 SSPFVPPILSSEE364 pKa = 4.32 YY365 pKa = 11.27 VNTLWFRR372 pKa = 11.84 YY373 pKa = 9.48 KK374 pKa = 10.43 FYY376 pKa = 10.7 FQIGGASISRR386 pKa = 11.84 EE387 pKa = 4.1 AISWPLRR394 pKa = 11.84 EE395 pKa = 4.35 TTDD398 pKa = 3.14 NTVPCGVAGCPYY410 pKa = 10.04 CIKK413 pKa = 10.82 KK414 pKa = 10.71 GDD416 pKa = 3.9 TDD418 pKa = 3.14 EE419 pKa = 5.77 HH420 pKa = 6.74 GILKK424 pKa = 10.18 EE425 pKa = 3.91 KK426 pKa = 10.42 ALEE429 pKa = 4.76 RR430 pKa = 11.84 ITEE433 pKa = 4.09 SPEE436 pKa = 3.61 RR437 pKa = 11.84 RR438 pKa = 11.84 KK439 pKa = 10.36 KK440 pKa = 10.68 RR441 pKa = 11.84 LVEE444 pKa = 3.91 KK445 pKa = 10.55 LSRR448 pKa = 11.84 ALRR451 pKa = 11.84 QRR453 pKa = 11.84 RR454 pKa = 11.84 KK455 pKa = 9.83 RR456 pKa = 11.84 KK457 pKa = 9.98 RR458 pKa = 11.84 ITWWDD463 pKa = 3.2 EE464 pKa = 3.69 GKK466 pKa = 10.53 AEE468 pKa = 4.35 EE469 pKa = 4.91 YY470 pKa = 10.74 VGTPHH475 pKa = 6.66 THH477 pKa = 6.64 PKK479 pKa = 9.71 NFKK482 pKa = 8.76 NKK484 pKa = 9.92 CIRR487 pKa = 11.84 RR488 pKa = 11.84 LATRR492 pKa = 11.84 LGLL495 pKa = 3.83
Molecular weight: 58.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.677
IPC_protein 10.248
Toseland 10.555
ProMoST 10.189
Dawson 10.672
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.994
Grimsley 10.73
Solomon 10.73
Lehninger 10.701
Nozaki 10.526
DTASelect 10.35
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.599
Patrickios 10.657
IPC_peptide 10.745
IPC2_peptide 9.209
IPC2.peptide.svr19 8.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
677
90
495
225.7
26.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.25 ± 0.29
1.625 ± 0.683
5.318 ± 1.03
5.022 ± 0.87
3.693 ± 0.926
8.124 ± 2.352
2.659 ± 0.451
4.727 ± 0.974
7.533 ± 0.793
7.681 ± 0.278
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.363 ± 0.385
4.284 ± 0.772
6.204 ± 1.044
4.431 ± 1.351
9.306 ± 2.941
6.647 ± 2.53
6.056 ± 0.169
3.693 ± 0.49
4.284 ± 0.291
3.102 ± 0.98
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here