Paenibacillus sp. OV191

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5948 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V4XFS5|A0A2V4XFS5_9BACL Putative adhesin OS=Paenibacillus sp. OV191 OX=2135473 GN=C7489_102316 PE=4 SV=1
MM1 pKa = 7.65SINSALLFDD10 pKa = 5.46FNDD13 pKa = 3.78AQSAALACEE22 pKa = 4.16TLRR25 pKa = 11.84EE26 pKa = 4.05LGYY29 pKa = 9.23EE30 pKa = 4.25TVLHH34 pKa = 7.15DD35 pKa = 4.19DD36 pKa = 3.46SRR38 pKa = 11.84MHH40 pKa = 5.43VHH42 pKa = 7.11LEE44 pKa = 4.08GSDD47 pKa = 3.35LTSALEE53 pKa = 3.74IAQAQGGQLVEE64 pKa = 4.25QSHH67 pKa = 6.65IDD69 pKa = 3.49ANAMTDD75 pKa = 3.09SAYY78 pKa = 11.07SLDD81 pKa = 4.69AISIPAHH88 pKa = 6.16LVNEE92 pKa = 4.3DD93 pKa = 2.84WTADD97 pKa = 3.59GQAEE101 pKa = 4.56YY102 pKa = 11.16ADD104 pKa = 3.71QLHH107 pKa = 6.2NRR109 pKa = 11.84DD110 pKa = 3.94DD111 pKa = 4.07DD112 pKa = 4.46ADD114 pKa = 3.88YY115 pKa = 11.32KK116 pKa = 11.47EE117 pKa = 4.94EE118 pKa = 3.99FLPDD122 pKa = 3.25SGTYY126 pKa = 10.59DD127 pKa = 3.23HH128 pKa = 7.26FSGDD132 pKa = 3.27VRR134 pKa = 11.84II135 pKa = 4.31

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V4WXR2|A0A2V4WXR2_9BACL Diadenosine tetraphosphate (Ap4A) HIT family hydrolase OS=Paenibacillus sp. OV191 OX=2135473 GN=C7489_110207 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.64RR12 pKa = 11.84AKK14 pKa = 8.72VHH16 pKa = 5.43GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.84VLSAA44 pKa = 4.11

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5948

0

5948

1929336

25

3359

324.4

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.238 ± 0.039

0.767 ± 0.01

5.125 ± 0.026

6.631 ± 0.032

4.256 ± 0.025

7.35 ± 0.033

2.097 ± 0.014

6.952 ± 0.033

5.524 ± 0.029

9.948 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.016

4.039 ± 0.022

3.999 ± 0.02

3.841 ± 0.023

4.872 ± 0.027

6.414 ± 0.028

5.311 ± 0.024

6.896 ± 0.026

1.369 ± 0.016

3.547 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski